LOCUS       sequence-23535-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Takafuji V, Forgues M, Unsworth E, Goldsmith P, Wang XW
  TITLE     An osteopontin fragment is essential for tumor cell invasion in 
            hepatocellular carcinoma.
  JOURNAL   Oncogene. 2007 Sep 27. 26(44):6361-71.
  PUBMED    17452979
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2007 Sep 27. 26(44):6361-71."
COMMENT     Sequence Label: pDest490-OPN-CT
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(391..415)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             435..458
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcgtcggacc agtttgtcaa aaagttggca ccatgagaat tgcagtgatt tgcttttgcc
       61 tcctaggcat cacctgtgcc ctgaacgcgc cttctgattg ggacagccgt gggaaggaca
      121 gttatgaaac gagtcagctg gatgaccaga gtgctgaaac ccacagccac aagcagtcca
      181 gattatataa gcggaaagcc aatgatgaga gcaatgagca ttccgatgtg attgatagtc
      241 aggaactttc caaagtcagc cgtgaattcc acagccatga atttcacagc catgaagata
      301 tgctggttgt agaccccaaa agtaaggaag aagataaaca cctgaaattt cgtatttctc
      361 atgaattaga tagtgcatct tctgaggtca atccaacttt cttgtacaaa gtggttcgat
      421 ctagaatggc tagcgactac aaggacgacg atgacaagta gtaatcgagt acaactataa
      481 ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa
      541 aattcgccac aacattgaag atggatccgt tcaactagca gaccattatc aacaaaatac
      601 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga cacaatctgc
      661 cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt ttgtannggg
      721 gcngnnntcc gggggcanga tgacctcaca atatgatnan cccctgannn ctcganggct
      781 tcagtgcngc tcgtgtgcct cccggctctg ncngaggccn cnngctcnaa aaggatgcc
//
