LOCUS       sequence-235350-        8682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of wtNmeCas9 with 3X NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, 
            Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller
            CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ
  TITLE     NmeCas9 is an intrinsically high-fidelity genome-editing platform.
  JOURNAL   Genome Biol. 2018 Dec 5;19(1):214. doi: 10.1186/s13059-018-1591-1.
  PUBMED    30518407
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-12-5"; volume: "19(1):214. doi"; pages: " 
            10.1186/s13059-018-1591-1"
COMMENT     Sequence Label: NmeCas9_3XNLS_pMCSG7
FEATURES             Location/Qualifiers
     source          1..8682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        285..388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(389..457,458..1249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(607..626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1420..2008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1909..1928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2162..2179
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2194..2333
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2259..2278)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2419..2441
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2435..2626)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3435..4517)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4479..4498
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4518..4595)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4801..4820
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4908..4927
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4908..4926
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4927..4951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4966..4988
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4999..5016
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5041..5061
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             5062..5082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5083..8325
                     /codon_start=1
                     /product="Cas9 endonuclease from the Neisseria meningitidis
                     Type II CRISPR/Cas system (Esvelt et al., 2013)"
                     /label=NmCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="AAFKPNSINYILGLDIGIASVGWAMVEIDEEENPIRLIDLGVRVF
                     ERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRTRRLLKREGVLQAANFDENGLIKS
                     LPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVAG
                     NAHALQTGDFRTPAELALNKFEKESGHIRNQRSDYSHTFSRKDLQAELILLFEKQKEFG
                     NPHVSGGLKEGIETLLMTQRTALSGDAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLTK
                     LNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKGLRYGKDN
                     AEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRLK
                     DRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDHYGKKNTE
                     EKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSFKDRKE
                     IEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKCLYSGKEINLGR
                     LNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFNGKDNSREWQEFK
                     ARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKRV
                     FASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNAFD
                     GKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTLEKLRTLLAEK
                     LSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKDL
                     EKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQKT
                     GVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGKDEEDWQL
                     IDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILEG
                     IGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     CDS             8371..8397
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             8410..8457
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8526..8543
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8596..8614)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8610..8657
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      301 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      361 tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      421 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      481 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      541 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      601 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      661 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      721 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      781 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      841 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      901 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      961 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1021 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1081 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1141 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1201 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1261 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1321 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1381 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1441 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1501 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1561 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1621 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1681 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1741 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1801 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1861 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1921 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1981 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2041 ggccttttgc tggccttttg ctcacatgtt ctttcttgcg ttatcccctg attctgtgga
     2101 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2161 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2221 tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca
     2281 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     2341 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     2401 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     2461 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt
     2521 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
     2581 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
     2641 aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga
     2701 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg
     2761 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta
     2821 atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca
     2881 taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga
     2941 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagttgctt cacgttcgct
     3001 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc
     3061 tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct
     3121 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
     3181 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
     3241 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga
     3301 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt
     3361 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
     3421 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3481 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
     3541 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
     3601 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     3661 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc
     3721 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
     3781 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
     3841 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
     3901 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
     3961 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
     4021 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
     4081 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
     4141 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
     4201 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     4261 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
     4321 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     4381 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
     4441 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
     4501 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
     4561 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg
     4621 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     4681 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     4741 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     4801 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     4861 gatgcgtccg gcgtagagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac
     4921 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
     4981 gaaggagata tacatatgca ccatcatcat catcattctt ctggtgtaga tctgggtacc
     5041 gagaacctgt acttccaatc ccctaagaag aagagaaagg tggctgcctt caaacctaat
     5101 tcaatcaact acatcctcgg cctcgatatc ggcatcgcat ccgtcggctg ggcgatggta
     5161 gaaattgacg aagaagaaaa ccccatccgc ctgattgatt tgggcgtgcg cgtatttgag
     5221 cgtgccgaag taccgaaaac aggcgactcc cttgccatgg caaggcgttt ggcgcgcagt
     5281 gttcgccgcc tgacccgccg tcgcgcccac cgcctgcttc ggacccgccg cctattgaaa
     5341 cgcgaaggcg tattacaagc cgccaatttt gacgaaaacg gcttgattaa atccttaccg
     5401 aatacaccat ggcaacttcg cgcagccgca ttagaccgca aactgacgcc tttagagtgg
     5461 tcggcagtct tgttgcattt aatcaaacat cgcggctatt tatcgcaacg gaaaaacgag
     5521 ggcgaaactg ccgataagga gcttggcgct ttgcttaaag gcgtagccgg caatgcccat
     5581 gccttacaga caggcgattt ccgcacaccg gccgaattgg ctttaaataa atttgagaaa
     5641 gaaagcggcc atatccgcaa tcagcgcagc gattattcgc atacgttcag ccgcaaagat
     5701 ttacaggcgg agctgatttt gctgtttgaa aaacaaaaag aatttggcaa tccgcatgtt
     5761 tcaggcggcc ttaaagaagg tattgaaacc ctactgatga cgcaacgcac tgccctgtcc
     5821 ggcgatgccg ttcaaaaaat gttggggcat tgcaccttcg aaccggcaga gccgaaagcc
     5881 gctaaaaaca cctacacagc cgaacgtttc atctggctga ccaagctgaa caacctgcgt
     5941 attttagagc aaggcagcga gcggccattg accgataccg aacgcgccac gcttatggac
     6001 gagccataca gaaaatccaa actgacttac gcacaagccc gtaagctgct gggtttagaa
     6061 gataccgcct ttttcaaagg cttgcgctat ggtaaagaca atgccgaagc ctcaacattg
     6121 atggaaatga aggcctacca tgccatcagc cgtgcactgg aaaaagaagg attgaaagac
     6181 aaaaaatccc cattaaacct ttctcccgaa ttacaagacg aaatcggcac ggcattctcc
     6241 ctgttcaaaa ccgatgaaga cattacaggc cgtctgaaag accgtataca gcccgaaatc
     6301 ttagaagcgc tgttgaaaca catcagcttc gataagttcg tccaaatttc cttgaaagca
     6361 ttgcgccgaa ttgtgcctct aatggaacaa ggcaaacgtt acgatgaagc ctgcgccgaa
     6421 atctacggag accattacgg caagaagaat acggaagaaa agatttatct gccgccgatt
     6481 cccgccgacg aaatccgcaa ccccgtcgtc ttgcgcgcct tatctcaagc acgtaaggtc
     6541 attaacggcg tggtacgccg ttacggctcc ccagctcgta tccatattga aactgcaagg
     6601 gaagtaggta aatcgtttaa agaccgcaaa gaaattgaga aacgccaaga agaaaaccgc
     6661 aaagaccggg aaaaagccgc cgccaaattc cgagagtatt tccccaattt tgtcggagaa
     6721 cccaaatcca aagatattct gaaactgcgc ctgtacgagc aacaacacgg caaatgcctg
     6781 tattcgggca aagaaatcaa cttaggccgt ctgaacgaaa aaggctatgt cgaaatcgac
     6841 catgccctgc cgttctcgcg cacatgggac gacagtttca acaataaagt actggtattg
     6901 ggcagcgaaa accaaaacaa aggcaatcaa accccttacg aatacttcaa cggcaaagac
     6961 aacagccgcg aatggcagga atttaaagcg cgtgtcgaaa ccagccgttt cccgcgcagt
     7021 aaaaaacaac ggattctgct gcaaaaattc gatgaagacg gctttaaaga acgcaatctg
     7081 aacgacacgc gctacgtcaa ccgtttcctg tgtcaatttg ttgccgaccg tatgcggctg
     7141 acaggtaaag gcaagaaacg tgtctttgca tccaacggac aaattaccaa tctgttgcgc
     7201 ggcttttggg gattgcgcaa agtgcgtgcg gaaaacgacc gccatcacgc cttggacgcc
     7261 gtcgtcgttg cctgctcgac cgttgccatg cagcagaaaa ttacccgttt tgtacgctat
     7321 aaagagatga acgcgtttga cggtaaaacc atagacaaag aaacaggaga agtgctgcat
     7381 caaaaaacac acttcccaca accttgggaa tttttcgcac aagaagtcat gattcgcgtc
     7441 ttcggcaaac cggacggcaa acccgaattc gaagaagccg ataccctaga aaaactgcgc
     7501 acgttgcttg ccgaaaaatt atcatctcgc cccgaagccg tacacgaata cgttacgcca
     7561 ctgtttgttt cacgcgcgcc caatcggaag atgagcgggc aagggcatat ggagaccgtc
     7621 aaatccgcca aacgactgga cgaaggcgtc agcgtgttgc gcgtaccgct gacacagtta
     7681 aaactgaaag acttggaaaa aatggtcaat cgggagcgcg aacctaagct atacgaagca
     7741 ctgaaagcac ggctggaagc acataaagac gatcctgcca aagcctttgc cgagccgttt
     7801 tacaaatacg ataaagcagg caaccgcacc caacaggtaa aagccgtacg cgtagagcaa
     7861 gtacagaaaa ccggcgtatg ggtgcgcaac cataacggta ttgccgacaa cgcaaccatg
     7921 gtgcgcgtag atgtgtttga gaaaggcgac aagtattatc tggtaccgat ttacagttgg
     7981 caggtagcga aagggatttt gccggatagg gctgttgtac aaggaaaaga tgaagaagat
     8041 tggcaactta ttgatgatag tttcaacttt aaattctcat tacaccctaa tgatttagtc
     8101 gaggttataa caaaaaaagc tagaatgttt ggttactttg ccagctgcca tcgaggcaca
     8161 ggtaatatca atatacgcat tcatgatctt gatcataaaa ttggcaaaaa tggaatactg
     8221 gaaggtatcg gcgtcaaaac cgccctttca ttccaaaaat accaaattga cgaactgggc
     8281 aaagaaatca gaccatgccg tctgaaaaaa cgcccgcctg tccgtggcgg gggtggttca
     8341 ggtggaggag ggtcaggcgg agggggttcg ccagcagcga agaaaaagaa gctggatggc
     8401 gggggatcta agcgcccagc cgctacaaaa aaagcgggac aggcgaagaa gaagaagtaa
     8461 aacattggaa gtggataacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac
     8521 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
     8581 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     8641 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at
//
