LOCUS       sequence-235357-        8753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression, Genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Teng F, Cui T, Feng G, Guo L, Xu K, Gao Q, Li T, Li J, Zhou Q, Li W
  TITLE     Repurposing CRISPR-Cas12b for mammalian genome engineering.
  JOURNAL   Cell Discov. 2018 Nov 27;4:63. doi: 10.1038/s41421-018-0069-3. 
            eCollection 2018.
  PUBMED    30510770
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2018-11-27"; volume: "4:63. doi"; pages: " 
            10.1038/s41421-018-0069-3. eCollection 2018"
COMMENT     Sequence Label: pCAG-AkCas12b-2AeGFP
FEATURES             Location/Qualifiers
     source          1..8753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        389..665
                     /label=chicken beta-actin promoter
     intron          666..1682
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1606..1628
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1690..1709
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1743..1761
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1773..1862
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             1869..1889
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5343..5363
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5385..5438
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5439..5441,5442..5444,5445..6158)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5439..5441,5442..5444,5445..6155)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5484..5505)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5745..5764)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6092..6113
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6201..6322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6238..6257)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6292..6311
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6365..6381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6365..6381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6378..6400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6389..6405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6413..6419,6420..6437,6438..6443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6458..6479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6596..6613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6767..7355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6847..6866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7526..8317,8318..8386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8149..8168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8387..8491)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8570..8589)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
      421 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
      481 gcagcgatgg gggcgggggg ggggggggns gcgcgccrgg cggggcgggg cggggcgrgg
      541 ggsggggsgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      661 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
      721 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
      781 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
      841 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
      901 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
      961 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     1021 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     1081 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     1141 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     1201 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
     1261 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
     1321 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     1381 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     1441 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1501 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     1561 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     1621 gtgtgaccgg cggctctagc gcctctgcta accatgttca tgccttcttc tttttcctac
     1681 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gctagtgaat
     1741 tctaatacga ctcactatag gccgccacca tgtaccctta tgatgttcca gattatgccg
     1801 gatacccata cgatgtccct gactatgcag gctcctaccc ttatgacgtc ccagactacg
     1861 ccaccggtcc caagaagaag aggaaggttc ccgggatggc cgtgaagagc atcaaggtga
     1921 agctgcgcct gagcgagtgc cccgacatcc tggccggcat gtggcagctg caccgcgcca
     1981 ccaacgccgg cgtgcgctac tacaccgagt gggtgagcct gatgcgccag gagatcctgt
     2041 acagccgcgg ccccgacggc ggccagcagt gctacatgac cgccgaggac tgccagcgcg
     2101 agctgctgcg ccgcctgcgc aaccgccagc tgcacaacgg ccgccaggac cagcccggca
     2161 ccgacgccga cctgctggcc atcagccgcc gcctgtacga gatcctggtg ctgcagagca
     2221 tcggcaagcg cggcgacgcc cagcagatcg ccagcagctt cctgagcccc ctggtggacc
     2281 ccaacagcaa gggcggccgc ggcgaggcca agagcggccg caagcccgcc tggcagaaga
     2341 tgcgcgacca gggcgacccc cgctgggtgg ccgcccgcga gaagtacgag cagcgcaagg
     2401 ccgtggaccc cagcaaggag atcctgaaca gcctggacgc cctgggcctg cgccccctgt
     2461 tcgccgtgtt caccgagacc taccgcagcg gcgtggactg gaagcccctg ggcaagagcc
     2521 agggcgtgcg cacctgggac cgcgacatgt tccagcaggc cctggagcgc ctgatgagct
     2581 gggagagctg gaaccgccgc gtgggcgagg agtacgcccg cctgttccag cagaagatga
     2641 agttcgagca ggagcacttc gccgagcaga gccacctggt gaagctggcc cgcgccctgg
     2701 aggccgacat gcgcgccgcc agccagggct tcgaggccaa gcgcggcacc gcccaccaga
     2761 tcacccgccg cgccctgcgc ggcgccgacc gcgtgttcga gatctggaag agcatccccg
     2821 aggaggccct gttcagccag tacgacgagg tgatccgcca ggtgcaggcc gagaagcgcc
     2881 gcgacttcgg cagccacgac ctgttcgcca agctggccga gcccaagtac cagcccctgt
     2941 ggcgcgccga cgagaccttc ctgacccgct acgccctgta caacggcgtg ctgcgcgacc
     3001 tggagaaggc ccgccagttc gccaccttca ccctgcccga cgcctgcgtg aaccccatct
     3061 ggacccgctt cgagagcagc cagggcagca acctgcacaa gtacgagttc ctgttcgacc
     3121 acctgggccc cggccgccac gccgtgcgct tccagcgcct gctggtggtg gagagcgagg
     3181 gcgccaagga gcgcgacagc gtggtggtgc ccgtggcccc cagcggccag ctggacaagc
     3241 tggtgctgcg cgaggaggag aagagcagcg tggccctgca cctgcacgac accgcccgcc
     3301 ccgacggctt catggccgag tgggccggcg ccaagctgca gtacgagcgc agcaccctgg
     3361 cccgcaaggc ccgccgcgac aagcagggca tgcgcagctg gcgccgccag cccagcatgc
     3421 tgatgagcgc cgcccagatg ctggaggacg ccaagcaggc cggcgacgtg tacctgaaca
     3481 tcagcgtgcg cgtgaagagc cccagcgagg tgcgcggcca gcgccgcccc ccctacgccg
     3541 ccctgttccg catcgacgac aagcagcgcc gcgtgaccgt gaactacaac aagctgagcg
     3601 cctacctgga ggagcacccc gacaagcaga tccccggcgc ccccggcctg ctgagcggcc
     3661 tgcgcgtgat gagcgtggac ctgggcctgc gcaccagcgc cagcatcagc gtgttccgcg
     3721 tggccaagaa ggaggaggtg gaggccctgg gcgacggccg ccccccccac tactacccca
     3781 tccacggcac cgacgacctg gtggccgtgc acgagcgcag ccacctgatc cagatgcccg
     3841 gcgagaccga gaccaagcag ctgcgcaagc tgcgcgagga gcgccaggcc gtgctgcgcc
     3901 ccctgttcgc ccagctggcc ctgctgcgcc tgctggtgcg ctgcggcgcc gccgacgagc
     3961 gcatccgcac ccgcagctgg cagcgcctga ccaagcaggg ccgcgagttc accaagcgcc
     4021 tgacccccag ctggcgcgag gccctggagc tggagctgac ccgcctggag gcctactgcg
     4081 gccgcgtgcc cgacgacgag tggagccgca tcgtggaccg caccgtgatc gccctgtggc
     4141 gccgcatggg caagcaggtg cgcgactggc gcaagcaggt gaagagcggc gccaaggtga
     4201 aggtgaaggg ctaccagctg gacgtggtgg gcggcaacag cctggcccag atcgactacc
     4261 tggagcagca gtacaagttc ctgcgccgct ggagcttctt cgcccgcgcc agcggcctgg
     4321 tggtgcgcgc cgaccgcgag agccacttcg ccgtggccct gcgccagcac atcgagaacg
     4381 ccaagcgcga ccgcctgaag aagctggccg accgcatcct gatggaggcc ctgggctacg
     4441 tgtacgaggc cagcggcccc cgcgagggcc agtggaccgc ccagcacccc ccctgccagc
     4501 tgatcatcct ggaggagctg agcgcctacc gcttcagcga cgaccgcccc cccagcgaga
     4561 acagcaagct gatggcctgg ggccaccgcg gcatcctgga ggagctggtg aaccaggccc
     4621 aggtgcacga cgtgctggtg ggcaccgtgt acgccgcctt cagcagccgc ttcgacgccc
     4681 gcaccggcgc ccccggcgtg cgctgccgcc gcgtgcccgc ccgcttcgtg ggcgccaccg
     4741 tggacgacag cctgcccctg tggctgaccg agttcctgga caagcaccgc ctggacaaga
     4801 acctgctgcg ccccgacgac gtgatcccca ccggcgaggg cgagttcctg gtgagcccct
     4861 gcggcgagga ggccgcccgc gtgcgccagg tgcacgccga catcaacgcc gcccagaacc
     4921 tgcagcgccg cctgtggcag aacttcgaca tcaccgagct gcgcctgcgc tgcgacgtga
     4981 agatgggcgg cgagggcacc gtgctggtgc cccgcgtgaa caacgcccgc gccaagcagc
     5041 tgttcggcaa gaaggtgctg gtgagccagg acggcgtgac cttcttcgag cgcagccaga
     5101 ccggcggcaa gccccacagc gagaagcaga ccgacctgac cgacaaggag ctggagctga
     5161 tcgccgaggc cgacgaggcc cgcgccaaga gcgtggtgct gttccgcgac cccagcggcc
     5221 acatcggcaa gggccactgg atccgccagc gcgagttctg gagcctggtg aagcagcgca
     5281 tcgagagcca caccgccgag cgcatccgcg tgcgcggcgt gggcagcagc ctggacgcta
     5341 gcccaaagaa gaagaggaaa gtctctagaa ggtccggcgg cggagagggc agaggaagtc
     5401 ttctaacatg cggtgacgtg gaggagaatc ccggcccaat ggtgagcaag ggcgaggagc
     5461 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     5521 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     5581 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     5641 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     5701 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     5761 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     5821 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     5881 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     5941 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     6001 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     6061 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     6121 ccgggatcac tctcggcatg gacgagctgt acaagtaact gcagcgcggg gatctcatgc
     6181 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6241 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6301 ccaaactcat caatgtatct tattaattaa cgtcgacctc tagctagagc ttggcgtaat
     6361 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6421 gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa
     6481 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6541 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6601 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6661 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6721 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6781 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6841 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6901 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6961 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7021 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7081 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7141 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7201 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7261 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7321 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7381 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7441 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7501 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7561 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7621 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7681 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7741 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7801 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7861 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7921 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7981 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8041 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8101 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8161 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8221 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8281 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8341 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8401 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8461 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8521 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct
     8581 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     8641 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     8701 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tat
//
