LOCUS       sequence-23536-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Takafuji V, Forgues M, Unsworth E, Goldsmith P, Wang XW
  TITLE     An osteopontin fragment is essential for tumor cell invasion in 
            hepatocellular carcinoma.
  JOURNAL   Oncogene. 2007 Sep 27. 26(44):6361-71.
  PUBMED    17452979
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2007 Sep 27. 26(44):6361-71."
COMMENT     Sequence Label: pDest490-OPN-NT
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(530..554)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             574..597
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgcgtcggac cagtttgtca aaaagttggc accatgagaa ttgcagtgat ttgcttttgc
       61 ctcctaggca tcacctgtgc cataccagtt aaacaggctg attctggaag ttctgaggaa
      121 aagcagcttt acaacaaata cccagatgct gtggccacat ggctaaaccc tgacccatct
      181 cagaagcaga atctcctagc cccacagaat gctgtgtcct ctgaagaaac caatgacttt
      241 aaacaagaga cccttccaag taagtccaac gaaagccatg accacatgga tgatatggat
      301 gatgaagatg atgatgacca tgtggacagc caggactcca ttgactcgaa cgactctgat
      361 gatgtagatg acactgatga ttctcaccag tctgatgagt ctcaccattc tgatgaatct
      421 gatgaactgg tcactgattt tcccacggac ctgccagcaa ccgaagtttt cactccagtt
      481 gtccccacag tagacacata tgatggccga ggtgatagtg tggtttatgg accaactttc
      541 ttgtacaaag tggttcgatc tagaatggct agcgactaca aggacgacga tgacaagtag
      601 taatcgagta caactataac tcacacaatg tatacatcac ggcagacaaa caaaagaatg
      661 gaatcaaagc taacttcaaa attcgccnga cttggaagag ggatccgttc actagcagac
      721 cattatcaac aaaatactcc antgggcgat ggcctgcttt tnacaaaaaa ctntnctngn
      781 cnannatctg cntttnaann nccaaaaaaa gngggac
//
