LOCUS       sequence-235371-       10183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLentiGuide is a gRNA insert lentiviral vector, with a stuffer 
            sequence for BsmBI cloning of annealed oligos corresponding to sgRNA
            sequence. It has Puromycin resistance and Ampicilin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Rubin AJ, Parker KR, Satpathy AT, Qi Y, Wu B, Ong AJ, Mumbach MR, Ji
            AL, Kim DS, Cho SW, Zarnegar BJ, Greenleaf WJ, Chang HY, Khavari PA
  TITLE     Coupled Single-Cell CRISPR Screening and Epigenomic Profiling 
            Reveals Causal Gene Regulatory Networks.
  JOURNAL   Cell. 2018 Dec 11. pii: S0092-8674(18)31514-9. doi: 
            10.1016/j.cell.2018.11.022.
  PUBMED    30580963
REFERENCE   2  (bases 1 to 10183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2018 
            Dec 11. pii: S0092-8674(18)31514-9. doi: 
            10.1016/j.cell.2018.11.022."
COMMENT     Sequence Label: pLentiGuide
FEATURES             Location/Qualifiers
     source          1..10183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1683..1916
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2101..2145
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2443..2560
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2699..2947
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2699..2719
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2870..2889
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        5039..5342
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5343..5546
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             5580..6176
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5580..5599)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6076..6096
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6186..6239
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6246..6255
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6252..6962
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6397..6415)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6665..6684
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(6982..7015)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7071..7659
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7124..7144)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7542..7553)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7662..7678)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7663..7679)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8188..8368
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8430..9018)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8510..8529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9189..9980,9981..10049))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9812..9831
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10050..10154)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     1561 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1621 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1681 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1741 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1801 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1861 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1921 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1981 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2041 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2101 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2161 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2221 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2281 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2341 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2401 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2461 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2521 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2581 cagagatcca gtttggttag taccgggccc gctctagaga tccgacgcgc catctctagg
     2641 cccgcgccgg ccccctcgca cggacttgtg ggagaagctc ggctactccc ctgccagaga
     2701 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     2761 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     2821 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     2881 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     2941 aaacaccgga gacggttgta aatgagcaca caaaatacac atgctaaaat attatattct
     3001 atgaccttta taaaatcaac caaaatcttc tttttaataa ctttagtatc aataattaga
     3061 atttttatgt tcctttttgc aaacttttaa taaaaatgag caaaataaaa aaacgctagt
     3121 tttagtaact cgcgttgttt tcttcacctt taataatagc tactccacca cttgttccta
     3181 agcggtcagc tcctgcttca atcatttttt gagcatcttc aaatgttcta actccaccag
     3241 ctgctttaac taaagcattg tctttaacaa ctgacttcat tagtttaaca tcttcaaatg
     3301 ttgcacctga ttttgaaaat cctgttgatg ttttaacaaa ttctaatcca gcttcaacag
     3361 ctatttcaca agctttcatg atttcttctt ttgttaataa acaattttcc ataatacatt
     3421 taacaacatg tgatccagct gcttttttta cagctttcat gtcttctaaa actaattcat
     3481 aatttttgtc ttttaatgca ccaatattta ataccatatc aatttctgtt gcaccatctt
     3541 taattgcttc agaaacttcg aatgcttttg tagctgttgt gcatgcacct agaggaaaac
     3601 ctacaacatt tgttattcct acatttgtgc cttttaataa ttctttacaa tagcttgttc
     3661 aatatgaatt aacacaaact gttgcaaaat caaattcaat tgcttcatca cataattgtt
     3721 taatttcagc tttcgtagca tcttgtttta ataatgtgtg atctatatat ttgtttagtt
     3781 tcattttttc tcctatatat tcatttttaa ttttaattct ttaataattt cgtctacttt
     3841 aactttagcg ttttgaacag attcaccaac acctataaaa taaattttta gtttaggttc
     3901 agttccactt gggcgaacag caaatcatga cttatcttct aaataaaatt ttagtaagtc
     3961 ttgtcctggc atattataca ttccatcgat gtagtcttca acattaacaa ctttaagtcc
     4021 agcaatttga gttaagggtg ttgctctcaa tgatttcatt aatggttcaa tttttaattt
     4081 cttttcttct ggtttaaaat tcaagtttaa agtgaaagtg taatatgcac ccatttcttt
     4141 aaataaatct tctaaatagt ctactaatgt tttattttgt tttttataaa atcaagcagc
     4201 ctctgctatt aatatagaag cttgtattcc atctttatct ctagctgagt catcaattac
     4261 atatccataa ctttcttcat aagcaaaaac aaaatttaat ccgttatctt cttctttagc
     4321 aatttctcta cccattcatt taaatccagt taaagttttt acaatattaa ctccatattt
     4381 ttcatgagcg attctatcac ccaaatcact tgttacaaaa cttgaatata gagccggatt
     4441 ttttggaatg ctatttaagc gttttagatt tgataatttt caatcaatta aaattggtcc
     4501 tgtttgattt ccatctaatc ttacaaaatg accatcatgt tttattgcca ttccaaatct
     4561 gtcagcatct gggtcattca taataataat atctgcatca tgtttaatac catattcaag
     4621 cggtattttt catgcaggat caaattctgg atttggattt acaacatttt taaatgtttc
     4681 atcttcaaat gcatgctctt caacctcaat aacgttatat cctgattcac gtaatatttt
     4741 tggggtaaat ttagttcctg ttccattaac tgcgctaaaa ataattttta aatctttttt
     4801 agcttcttgc tcttttttgt acgtctctgt ttaagagcta tgctggaaac agcatagcaa
     4861 gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     4921 tctcgagtac taggatccat taggcggccg cgtggataac cgtattaccg ccatgcatta
     4981 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     5041 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     5101 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     5161 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     5221 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     5281 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     5341 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     5401 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     5461 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     5521 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcccacca
     5581 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     5641 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     5701 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     5761 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     5821 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     5881 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     5941 ccgcgtggtt cctggccacc gtcggcgtat cgcccgacca ccagggcaag ggtctgggca
     6001 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     6061 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6121 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccggat
     6181 cgggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat cctggcccac
     6241 cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     6301 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     6361 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     6421 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     6481 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     6541 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     6601 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     6661 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     6721 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     6781 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     6841 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     6901 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     6961 aagaattcgt cgagggacct aataacttcg tatagcatac attatacgaa gttatacatg
     7021 tttaagggtt ccggttccac taggtacaat tcgatatcaa gcttatcgat aatcaacctc
     7081 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7141 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7201 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7261 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7321 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7381 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7441 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7501 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7561 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7621 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgat
     7681 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     7741 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     7801 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7861 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     7921 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     7981 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     8041 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     8101 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     8161 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     8221 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8281 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8341 ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa aaggccagca aaaggccagg
     8401 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8461 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8521 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8581 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8641 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8701 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8761 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8821 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8881 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8941 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9001 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9061 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9121 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9181 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9241 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9301 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9361 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9421 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9481 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9541 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9601 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9661 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9721 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9781 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9841 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9901 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9961 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10021 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10081 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10141 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acg
//
