LOCUS       sequence-235408-        2512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription terminator Cyc1TT of Y. lipolytica for GoldenMOCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Egermeier M, Sauer M, Marx H
  TITLE     Golden Gate based metabolic engineering strategy for wild-type 
            strains of Yarrowia lipolytica.
  JOURNAL   FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022.
  PUBMED    30698703
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022."
COMMENT     Sequence Label: BB1_34_YlCyc1TT
FEATURES             Location/Qualifiers
     source          1..2512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..22
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(813..829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(813..829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      877..908
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        909..1011
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1012..1827
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1085..1104)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1851..1878
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1890..2477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1970..1989)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatccagt cgcgctgctc
      181 tcgtcgatcc gaagacgcag cgtcaatcga accgcaacct ccacatgtct cagctgtgaa
      241 tggagacggt ttcaagacta ttagcgagct gggcaacaaa acaaccttct gttgctgtag
      301 atgtgtacat atatacagcg ccttgatttc gtcagccagt catgttgttt ggagtaagca
      361 gtacaggcga gcgtgcgagt acgaggtggt tggagatgga cgatagtgta agaaagaata
      421 caacacaaga cgcaagagaa gccgtcgccc cacggagtat cactcgtcct aatacaagcg
      481 ctcgtagaag ctgagtgtta gaacaactcg caactgattc tcgcaattga atctcgcgat
      541 tgaatctcgc aattgaatct cgcaagttgg gtagatccac ctcagtaaca gatcccattg
      601 tacaatacga accgcctacc gaccaaatct ttaaataatc aattgatata tacaggctaa
      661 gggtccagtt gtagacgcaa gcgcgtcttc ggtgcgagcg gatcgagcag tgtcgatcac
      721 tactggaccg cgagctgtgc tgcgacccgt gatcttacgg cattatacgt atgatcggtc
      781 cacgatcagc tagattatct agtcagcttg atgtcatagc tgtttcctga ggctcaatac
      841 tgaccattta aatcatacct gacctccata gcagaaagtc aaaagcctcc gaccggaggc
      901 ttttgacttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
      961 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatgagccat
     1021 attcaacggg aaacgtcttg cttgaagccg cgattaaatt ccaacatgga tgctgattta
     1081 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg
     1141 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1201 gatgttacag atgagatggt caggctaaac tggctgacgg aatttatgcc tcttccgacc
     1261 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatcccaggg
     1321 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1381 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacggc
     1441 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttggtgcg
     1501 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     1561 aaactcttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     1621 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     1681 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     1741 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     1801 cacttgatgc tcgatgagtt tttctaatga ggacctaaat gtaatcacct ggctcacctt
     1861 cgggtgggcc tttctgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1921 cacaaaaatc gatgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1981 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2041 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2101 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2161 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2221 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2281 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2341 ggtatctgcg ctctgctgaa gccagttacc tcggaaaaag agttggtagc tcttgatccg
     2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2461 gaaaaaaagg atctcaagaa gatcctttga ttttctaccg aagaaaggcc ca
//
