LOCUS       sequence-235416-        8101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8101)
  TITLE     Rehwinkel Lab plasmids
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.3/Capsid
FEATURES             Location/Qualifiers
     source          1..8101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    510..528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    590..614
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1020..1044)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1097..1138
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1162..1750
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1215..1235)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1633..1644)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        1765..2081
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1932..2067
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2129..2176
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2195..2593
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             2680..2913
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2985..3106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3022..3041)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3076..3095
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3146..3281
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3301..3320)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3302..3320)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3330..3347)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3330..3346)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3339..3361)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3488..3943
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3575..3594)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3785..3806
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3969..4073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4074..4142,4143..4934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4292..4311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5105..5693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5594..5613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5847..5864
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5981..6002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6017..6022,6023..6040,6041..6047)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6055..6071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6060..6082
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6079..6095
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6079..6095
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6114..6134
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6116..6134
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6162..6388
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6389..6569
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6616..6741
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7234..7467
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7652..7696
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7963..8080
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
      541 tgatagagat cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
      601 caaaaaagca ggctccgaat tcgcccttag gaggacagct atgaaaaacc caaaaaagaa
      661 atccggagga ttccggattg tcaatatgct aaaacgcgga gtagcccgtg tgagcccctt
      721 tgggggcttg aagaggctgc cagccggact tctgctgggt catgggccca tcaggatggt
      781 cttggcaatt ctagcctttt tgagattcac ggcaatcaag ccatcactgg gtctcatcaa
      841 tagatggggt tcagtgggga aaaaagaggc tatggaaata ataaagaagt tcaagaaaga
      901 tctggctgcc atgctgagaa taatcaatgc taggaaggag aagaagagac gaggcgcaga
      961 tactagtgtc ggaattgttg gcctcctgct gaccacagct atggcaaagg gcgaattcga
     1021 cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga
     1081 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
     1141 taccggttag taatgatcga caatcaacct ctggattaca aaatttgtga aagattgact
     1201 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     1261 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     1321 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     1381 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     1441 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     1501 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     1561 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     1621 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     1681 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     1741 gcctccccgc ctggcgatgg taccggtgtg gaaagtcccc aggctcccca gcaggcagaa
     1801 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     1861 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     1921 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     1981 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     2041 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     2101 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     2161 catagtataa tacgacaagg tgaggaacta aaccatggcc aagcctttgt ctcaagaaga
     2221 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta
     2281 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
     2341 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     2401 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
     2461 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
     2521 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
     2581 gtgggagggc taagcacaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     2641 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     2701 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     2761 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     2821 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     2881 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     2941 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     3001 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3061 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     3121 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3181 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3241 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     3301 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     3361 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     3421 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3481 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3541 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3601 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3661 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3721 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3781 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3841 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3901 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     3961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4921 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4981 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5221 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5761 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5821 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     5881 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     5941 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6001 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6061 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     6121 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     6181 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     6241 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     6301 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     6361 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     6421 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6481 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6541 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     6601 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     6661 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     6721 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     6781 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     6841 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     6901 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     6961 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7021 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     7081 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     7141 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     7201 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     7261 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     7321 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     7381 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     7441 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     7501 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     7561 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     7621 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     7681 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     7741 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     7801 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     7861 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     7921 atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg
     7981 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     8041 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgataagc ttgggagttc
     8101 c
//
