LOCUS       sequence-235419-        8831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB
  TITLE     Dynamic analysis of cytosolic glucose and ATP levels in yeast using 
            optical sensors.
  JOURNAL   Biochem J. 2010 Dec 1. 432(2):399-406.
  PUBMED    20854260
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2010 Dec 1. 432(2):399-406."
COMMENT     Sequence Label: pDR-GW AT1.03YEMK
FEATURES             Location/Qualifiers
     source          1..8831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(1021..1476)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1158..1179)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1370..1389
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    2284..2308
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2338..2340,2341..2343,2344..2856)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             3433..4167
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     protein_bind    complement(4205..4229)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4291..4310)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4292..4310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      4458..4645
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4513..4537)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4657..4674)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4657..4673)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4666..4688)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5109..6455
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(6566..7369)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7370..7585)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7610..7628)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7696..7800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7801..7869,7870..8661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8019..8038)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta ccgcatcagg
     1021 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     1081 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     1141 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     1201 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     1261 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     1321 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     1381 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     1441 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccaagct tcctgaaacg
     1501 gagaaacata aacaggcatt gctgggatca cccatacatc actctgtttt gcctgacctt
     1561 ttccggtaat ttgaaaacaa acccggtctc gaagcggaga tccggcgata attaccgcag
     1621 aaataaaccc atacacgaga cgtagaacca gccgcacatg gccggagaaa ctcctgcgag
     1681 aatttcgtaa actcgcgcgc attgcatctg tatttcctaa tgcggcactt ccaggcctcg
     1741 atcgagaccg tttatccatt gcttttttgt tgtctttttc cctcgttcac agaaagtctg
     1801 aagaagctat agtagaacta tgagcttttt ttgtttctgt tttccttttt ttttttttta
     1861 cctctgtgga aattgttact ctcacactct ttagttcgtt tgtttgtttt gtttattcca
     1921 attatgaccg gtgacgaaac gtggtcgatg gtgggtaccg cttatgctcc cctccattag
     1981 tttcgattat ataaaaaggc caaatattgt attattttca aatgtcctat cattatcgtc
     2041 taacatctaa tttctcttaa attttttctc tttctttcct ataacaccaa tagtgaaaat
     2101 ctttttttct tctatatcta caaaaacttt ttttttctat caacctcgtt gataaatttt
     2161 ttctttaaca atcgttaata attaattaat tggaaaataa ccattttttc tctcttttat
     2221 acacacattc aaaagaaaga aaaaaaatat accccagcct cgactagtga attcgcggcc
     2281 atcacaagtt tgtacaaaaa agcaggcttc gtttaaacgg gccctctaga ctcgagtatg
     2341 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2401 gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2461 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2521 gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     2581 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgtac catcttcttc
     2641 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2701 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2761 ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     2821 atcaaggccc acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
     2881 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2941 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     3001 ctggagttcg tgaccgccgc catcgatatg aaaactgtga aagtgaatat aacaacccct
     3061 gatgggccag tctacgacgc tgatatcgag atggtgtccg tgcgggccga gagtggtgat
     3121 ctcggcatcc tccccggtca cattcccaca aaggccccac tgaagatcgg agctgtgcgg
     3181 ctgaagaagg acggccaaac cgagtacgtc gcagtctcag gcggcactgt tgaagtgcgg
     3241 cctgaccacg ttaccattaa tgctcaagcc gctgaaacag ccgaaggaat cgacaaagag
     3301 agagcagaag ccgcaagaca gagggcccag gagcggctga actctcaatc cgatgacacc
     3361 gatattcgcc gggccgagct ggcactgcag agggccctga acagactgga cgtggctgaa
     3421 atgaaggaat tcatggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
     3481 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg
     3541 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3601 gggatcactc tcggcatgga cgagctgtac aagggtggca gcggtggcat ggtgagcaag
     3661 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3721 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     3781 ctgaagctga tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3841 ctgggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     3901 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     3961 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     4021 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     4081 aactacaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     4141 aacttcaaga tccgccacaa catcgagtaa gcttaagttt aaaccgctga tcagcctcga
     4201 ctgcacccag ctttcttgta caaagtggtg tagttaattc atgatggccg ctgcaggtcg
     4261 acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcggatc
     4321 cagctttgga cttcttcgcc agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt
     4381 ctaccttgcc agaaatttac gaaaagatgg aaaagggtca aatcgttggt agatacgttg
     4441 ttgacacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta
     4501 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
     4561 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg
     4621 ctcttattga ccacacctct accggcatgc caattcactg gccgtcgttt tacaacgtcg
     4681 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     4741 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     4801 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     4861 ccgcataatc ggatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag
     4921 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta
     4981 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa
     5041 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata
     5101 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc
     5161 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag
     5221 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag
     5281 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     5341 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc
     5401 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct
     5461 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc
     5521 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact
     5581 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct
     5641 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca
     5701 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa
     5761 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt
     5821 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa
     5881 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag
     5941 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg
     6001 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc
     6061 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac
     6121 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
     6181 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
     6241 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
     6301 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
     6361 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
     6421 tccttcaatg ctatcatttc ctttgatatt ggatcgatcc gatgataagc tgtcaaacat
     6481 gagaattggg taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac
     6541 atgcatttac ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt
     6601 cccagcctgc ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata
     6661 gtcctcttcc aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat
     6721 actgttgacc caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc
     6781 aatcgtaacc ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat
     6841 ctttgtcgct cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc
     6901 ccttgcatga caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg
     6961 ccgcctgctt caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt
     7021 ctgcccattc tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa
     7081 tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta
     7141 gcggcttaac tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta
     7201 aacaaatttt gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat
     7261 ccaatgaagc acacaagttt gtttgctttt cgtgcatgat attaaatagc ttggcagcaa
     7321 caggactagg atgagtagca gcacgttcct tatatgtagc tttcgacatg atttatcttc
     7381 gtttcctgca tgtttttgtt ctgtgcagtt gggttaagaa tactgggcaa tttcatgttt
     7441 cttcaacact acatatgcgt atatatacca atctaagtct gtgctccttc cttcgttctt
     7501 ccttctgttc ggagattacc gaatcaaaaa aatttcaagg aaaccgaaat caaaaaaaag
     7561 aataaaaaaa aaatgatgaa ttgaaaagct aattcttgaa gacgaaaggg cctcgtgata
     7621 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     7681 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7741 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7801 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7861 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7921 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7981 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8041 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8101 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8161 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8221 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8281 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8341 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8401 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8461 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8521 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8581 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8641 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8701 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8821 aaaggatctt c
//
