LOCUS       sequence-235427-       15008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tetracycline-inducible expression of untagged full-length RECK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15008)
  AUTHORS   Kumari R, Silic MR, Jones-Hall YL, Nin-Velez A, Yang JY, Mittal SK, 
            Zhang G
  TITLE     Identification of RECK as an evolutionarily conserved tumor 
            suppressor gene for zebrafish malignant peripheral nerve sheath 
            tumors.
  JOURNAL   Oncotarget. 2018 May 4;9(34):23494-23504. doi: 
            10.18632/oncotarget.25236. eCollection 2018 May 4.
  PUBMED    29805750
REFERENCE   2  (bases 1 to 15008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.25236"; journalName: "Oncotarget"; date: 
            "2018-05-4- 4"; volume: "9"; issue: "34"; pages: "23494-23504"
COMMENT     Sequence Label: pSlick-Neo-RECK
FEATURES             Location/Qualifiers
     source          1..15008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2725..2749)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2799..4010
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3847..3866
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4069..4686,4687..4692,4693..4728,4729..4731,4732..4767,
                     4768..4770,4771..4806,4807..4815)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4868..5441
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5035..5052)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5262..5281
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5457..6251
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5457..6251
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5511..5530)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6121..6140
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6419..7007
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6472..6492)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6890..6901)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7010..7026)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7011..7027)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7536..7716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7742..7759)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7748..7972
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8018..8446
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8105..8124)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8315..8336
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8455..8475)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8460..8789
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8640..8775
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8702..8721
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8837..8884
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8903..9277
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9407..9528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9444..9463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9498..9517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9577..9593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9577..9593)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9590..9612)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9601..9617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9625..9631,9632..9649,9650..9655))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9670..9691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9808..9825)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9979..10567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10059..10078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10738..11529,11530..11598))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11361..11380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11599..11703)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11778..11797)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11969..12348
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12350..12548
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             12566..12746
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12793..12918
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13411..13644
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13829..13873
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    14171..14288
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    14345..14369
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        14401..14715
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    14409..14427
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    14445..14463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    14480..14498
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    14516..14534
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    14552..14570
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    14587..14605
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    14623..14641
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     14688..14712
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggttgaaa tttggaattg tatgaattca tctttgccag gtgtgtttaa gaagtctgat
       61 ggctgggttg gcttaggctg ctgtgaactg gctattgcct tggagtgtcg acaggcatgc
      121 aagcaggcat cttcaaagaa tgatatttcc aaagtttgca gaaaagaata tgagaatgct
      181 cttttcagtt gcattagcag aaatgaaatg ggctcggttt gttgcagtta tgcaggtcat
      241 cacacaaact gccgagaata ctgtcaagcc atttttcgaa cagactcttc tcctggtcca
      301 tctcagataa aagcagtgga aaattattgc gcctctatta gtccacaatt aatacattgt
      361 gtgaacaatt atactcaatc ttatccaatg aggaacccaa cggatagttt atattgctgt
      421 gacagagctg aagaccatgc ttgccaaaat gcctgcaaga ggatcctgat gtccaagaaa
      481 acggaaatgg agattgttga tggtctcatc gagggttgta agacccagcc cttgcctcaa
      541 gatcctcttt ggcaatgttt tcttgaaagc tcacaatctg ttcaccctgg agtcactgta
      601 caccctcctc cctctacagg cctcgatggg gctaaattgc attgttgttc taaagcaaac
      661 acttcaacat gtagggaact ctgcactaaa ctttacagca tgagctgggg caatacacag
      721 agttggcaag agtttgatcg cttttgtgaa tataatccag tggaagtgtc catgttgacc
      781 tgtttagcgg atgtccggga accttgccag ttgggctgta gaaaccttac ttactgtact
      841 aattttaaca acaggccaac agaacttttc aggagttgta atgcacagtc agatcaagga
      901 gccatgaatg acatgaagtt gtgggagaaa ggaagcataa agatgccatt tatcaatata
      961 cctgttcttg atattaaaaa gtgccagcca gagatgtgga aagcaatagc ttgttcactg
     1021 cagattaaac cttgtcatag taaatctcgg ggaagtatta tttgcaaatc agattgtgtg
     1081 gagattctta aaaaatgtgg agaccagaac aaattccctg aagaccacac agctgaaagt
     1141 atttgtgagc ttctgtcacc tacagatgat ctgaagaatt gtataccttt ggatacatac
     1201 ctcaggccaa gtactttagg taacattgta gaagaagtga ctcatccctg taacccaaat
     1261 ccttgccctg ccaatgagct ctgtgaagta aaccgaaaag gatgtccatc tggagatccc
     1321 tgtcttccgt acttttgtgt tcaaggttgc aaactgggag aagcttctga tttcattgtc
     1381 cgtcaaggga cactaatcca ggtgccatca tctgcagggg aagttggttg ttataaaatc
     1441 tgttcatgtg gacaaagtgg actcttagaa aactgtatgg aaatgcactg tatagacctc
     1501 cagaagtctt gtattgttgg aggaaaaaga aaaagtcatg gaacatcctt tagtattgac
     1561 tgcaatgtct gttcttgttt tgctggcaat ttggtgtgct ctacccgcct ttgcctcagt
     1621 gagcacagtt cagaagatga ccgacgtacc ttcacaggtc tgccctgtaa ctgtgcagat
     1681 cagtttgtcc ctgtatgtgg gcagaatggg cgcacttacc ccagtgcctg cattgctcgc
     1741 tgtgtgggcc tccaagacca tcagtttgag tttggatcat gcatgtcaaa ggatccatgt
     1801 aatcctaatc cctgccaaaa aaaccaaagg tgcataccca aaccacaggt ctgcctgacg
     1861 acttttgata aatttggatg tagccagtat gagtgtgtac caagacagct cgcgtgtgac
     1921 caggtccaag atcctgtttg tgacacagac cacatggagc acaacaatct ctgcacttta
     1981 taccaaagag gaaaaagcct ctcttacaaa ggtccctgcc agcccttttg cagagcaacc
     2041 gagcccgtat gtgggcacaa tggtgagacc tacagcagtg tgtgtgctgc ctactcggat
     2101 cgcgtggcag tcgattacta tggggactgc caggccgtcg gagtcctctc agagcacagc
     2161 tccgtcgccg agtgtgcttc tgtcaagtgt ccttcgctct tggcagctgg atgcaaaccc
     2221 atcatcccac cgggtgcttg ttgcccatta tgtgctggga tgttaagagt tttatttgac
     2281 aaagaaaaac tggatactat tgctaaggta acaaataaaa agccaataac agttctggaa
     2341 atacttcaga aaatccgcat gcacgtgtct gtcccacagt gtgatgtgtt tggatacttc
     2401 agcattgaat cagaaattgt gatcctgatc attcccgtcg atcactatcc aaaagctctg
     2461 cagattgaag cctgcaataa agaagcagag aagattgagt cccttatcaa ctctgacagc
     2521 ccgactttgg cgtcccatgt ccctctctct gccctcatca tttcccaggt acaggtctcc
     2581 agcagtgtgc catcggccgg tgtcagggcc aggccttctt gccactccct cctccttccc
     2641 ctcagcttgg gccttgcctt gcacttgctc tggacatata actgactgcc cacggaatct
     2701 tgctgaaaaa ctcgagatat ctagacccag ctttcttgta caaagtggtt gataacatct
     2761 cgagccataa agatggttaa ttaacccacc caagatctgg cctccgcgcc gggttttggc
     2821 gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag
     2881 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
     2941 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
     3001 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
     3061 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
     3121 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
     3181 ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc
     3241 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
     3301 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
     3361 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
     3421 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
     3481 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
     3541 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
     3601 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
     3661 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
     3721 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     3781 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     3841 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     3901 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     3961 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac gaagcttggt
     4021 accgagctcg gatctccacc ccgtaccggt cctgcagtcg aattcaccat gtctagactg
     4081 gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg tatcgaaggc
     4141 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
     4201 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat
     4261 acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
     4321 tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
     4381 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
     4441 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
     4501 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     4561 tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct
     4621 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     4681 agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     4741 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     4801 atgctccccg ggtaactaag taaggatccg cggccgcact agaggaattc cgcccctctc
     4861 cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     4921 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     4981 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     5041 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     5101 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     5161 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     5221 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     5281 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     5341 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     5401 accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttaccgg tccaccatga
     5461 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5521 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5581 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     5641 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5701 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5761 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5821 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5881 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5941 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     6001 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     6061 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     6121 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     6181 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     6241 agttcttctg atgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     6301 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     6361 aaaatgaatg caattgttgt tgtttagtcc ctcccaattc gatatcaagc ttatcgataa
     6421 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6481 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6541 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6601 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6661 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6721 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6781 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6841 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6901 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6961 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     7021 acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     7081 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     7141 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7201 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     7261 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     7321 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     7381 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     7441 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     7501 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     7561 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7621 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7681 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     7741 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     7801 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     7861 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     7921 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     7981 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     8041 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8101 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8161 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8221 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8281 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8341 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8401 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     8461 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8521 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8581 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8641 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8701 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     8761 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     8821 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     8881 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     8941 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     9001 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     9061 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     9121 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     9181 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     9241 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     9301 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     9361 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     9421 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9481 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     9541 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9601 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9661 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9721 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9781 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9841 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9901 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9961 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10021 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10081 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10141 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10201 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10261 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10321 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10381 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    10441 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10501 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10561 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10621 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10681 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10741 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10801 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10861 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10921 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10981 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11041 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11101 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11161 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11221 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11281 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11341 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11401 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11461 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11521 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11581 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11641 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11701 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
    11761 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
    11821 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
    11881 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
    11941 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
    12001 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
    12061 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
    12121 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
    12181 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
    12241 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
    12301 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
    12361 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
    12421 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
    12481 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
    12541 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
    12601 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    12661 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    12721 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    12781 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    12841 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    12901 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    12961 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    13021 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    13081 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    13141 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    13201 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    13261 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
    13321 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
    13381 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
    13441 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
    13501 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
    13561 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
    13621 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
    13681 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
    13741 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
    13801 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
    13861 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
    13921 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
    13981 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
    14041 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
    14101 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
    14161 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    14221 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    14281 aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt ctagacagtt
    14341 atcaacaagt ttgtacaaaa aagcaggctt taaaggaacc aattcagtcg actggatccc
    14401 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
    14461 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
    14521 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
    14581 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
    14641 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
    14701 aaccgtcaga tcgccactag aaagatgccg cccggcccgg acatggcggg gggcctggct
    14761 ccgggcagtg cgggtgcatt gtgttgtaat cattcaaagg ataaccaaat gtgccgtgat
    14821 gtatgtgaac agttggtagt tttggaggaa aatggctaat aaataagcta actggagata
    14881 aatccttttt cactgtccat agcattcatg gattttaccg tctcagctat ttgcagattt
    14941 tctcctcaaa aagtgaatcc cgactaaaac atctgttgca gcgagcccca gattattgcc
    15001 cagagaca
//
