LOCUS       sequence-235531-       10967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of PYL-SfGFP-Emerin, lentivirus vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Wang H, Xu X, Nguyen CM, Liu Y, Gao Y, Lin X, Daley T, Kipniss NH, 
            La Russa M, Qi LS
  TITLE     CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear 
            Organization.
  JOURNAL   Cell. 2018 Nov 15;175(5):1405-1417.e14. doi: 
            10.1016/j.cell.2018.09.013. Epub 2018 Oct 11.
  PUBMED    30318144
REFERENCE   2  (bases 1 to 10967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.013"; journalName: "Cell"; date: 
            "2018-11-15- 15"; volume: "175"; issue: "5"; pages: "1405-1417.e14"
COMMENT     Sequence Label: pHR-SFFV-PYL1-sfGFP-Emerin
FEATURES             Location/Qualifiers
     source          1..10967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..62)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        47..376
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      227..362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1510..1575
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1705..1725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2150..2284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2187..2206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2241..2260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2452..3085
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3132..3257
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3754..3987
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4172..4216
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4501..4618
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4771..5178
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5748..6458
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             5748..6458
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(5775..5803)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6398..6418
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    7282..7870
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7335..7355)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7753..7764)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7873..7889)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7874..7890)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7980..8213
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8238..8256)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8239..8256)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8546..8565)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8779..8798)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8898..8920
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8958..8976)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9044..9148
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9149..9217,9218..10009)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9367..9386)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10180..10768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10669..10688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10922..10939
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgtcgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
       61 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      121 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      181 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
      241 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
      301 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
      361 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
      421 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
      481 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
      541 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
      601 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
      661 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
      721 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
      781 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
      841 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
      901 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
      961 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     1021 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     1081 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     1141 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     1201 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     1261 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     1321 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     1381 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
     1441 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1501 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1561 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     1621 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     1681 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     1741 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     1801 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     1861 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     1921 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     1981 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     2041 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     2101 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2161 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2221 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     2281 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     2341 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     2401 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
     2461 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     2521 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     2581 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     2641 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     2701 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     2761 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     2821 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     2881 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     2941 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3001 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3061 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     3121 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     3181 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     3241 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     3301 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     3361 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     3421 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     3481 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     3541 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     3601 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     3661 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     3721 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     3781 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     3841 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     3901 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     3961 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     4021 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     4081 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     4141 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     4201 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     4261 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     4321 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     4381 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     4441 atccattcga ttagtgaacg gatctcgacg gtatcgccaa atggcagtat tcatccacaa
     4501 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4561 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     4621 ggtttattac agggacagca gagatccagt ttggatcgat aagcttgata tcgaattcct
     4681 gcagccccga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     4741 cccacctgta ggtttggcaa gctagctgca gtaacgccat tttgcaaggc atggaaaaat
     4801 accaaaccaa gaatagagaa gttcagatca agggcgggta catgaaaata gctaacgttg
     4861 ggccaaacag gatatctgcg gtgagcagtt tcggccccgg cccggggcca agaacagatg
     4921 gtcaccgcag tttcggcccc ggcccgaggc caagaacaga tggtccccag atatggccca
     4981 accctcagca gtttcttaag acccatcaga tgtttccagg ctcccccaag gacctgaaat
     5041 gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt
     5101 ctgcttcccg agctctataa aagagctcac aacccctcac tcggcgcgcc agtcctccga
     5161 cagactgagt catgggtggg ggcgcgccaa ctcaagacga attcacccaa ctctcccaat
     5221 caatcgccga gttccacacg taccaactcg gtaacggccg ttgctcatct ctcctagctc
     5281 agcgaatcca cgcgccgccg gaaacagtat ggtccgtggt gagacgtttc gataggccac
     5341 agatttacaa acacttcatc aaaagctgta acgtgagtga agatttcgag atgcgagtgg
     5401 gatgcacgcg cgacgtgaac gtgataagtg gattaccggc gaatacgtct cgagagagat
     5461 tagatctgtt ggacgatgat cggagagtga ctgggtttag tataaccggt ggtgaacata
     5521 ggctgaggaa ttataaatcg gttacgacgg ttcatagatt tgagaaagaa gaagaagaag
     5581 aaaggatctg gaccgttgtt ttggaatctt atgttgttga tgtaccggaa ggtaattcgg
     5641 aggaagatac gagattgttt gctgatacgg ttattagatt gaatcttcag aaacttgctt
     5701 cgatcactga agctatgaac gtggatccag gtggaggtgg aagcggtagc aaaggagaag
     5761 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
     5821 aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc acccttaaat
     5881 ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact actctgacct
     5941 atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac tttttcaaga
     6001 gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggacct
     6061 acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa
     6121 agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgag tacaacttta
     6181 actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca
     6241 aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat caacaaaata
     6301 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg
     6361 tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg
     6421 ctgctgggat tacacatggc atggatgagc tctacaaagg tggaggtcgg accgaaatgg
     6481 acaactacgc agatctttcg gataccgagc tgaccacctt gctgcgccgg tacaacatcc
     6541 cgcacgggcc tgtagtagga tcaactcgta ggctttacga gaagaagatc ttcgagtacg
     6601 agacccagag gcggcggctc tcgcccccca gctcgtccgc cgcctcctct tatagcttct
     6661 ctgacttgaa ttcgactaga ggggatgcag atatgtatga tcttcccaag aaagaggacg
     6721 ctttactcta ccagagcaag ggctacaatg acgactacta tgaagagagc tacttcacca
     6781 ccaggactta tggggagccc gagtctgccg gcccgtccag ggctgtccgc cagtcagtga
     6841 cttcattccc agatgctgac gctttccatc accaggtgca tgatgacgat cttttgtctt
     6901 cttctgaaga ggagtgcaag gatagggaac gccccatgta cggccgggac agtgcctacc
     6961 agagcatcac gcactaccgc cctgtttcag cctccaggag ctccctggac ctgtcctatt
     7021 atcctacttc ctcctccacc tcttttatgt cctcctcatc atcttcctct tcatggctca
     7081 cccgccgtgc catccggcct gaaaaccgtg ctcctggggc tgggctgggc caggatcgcc
     7141 aggtcccgct ctggggccag ctgctgcttt tcctggtctt tgtgatcgtc ctcttcttca
     7201 tttaccactt catgcaggct gaagaaggca accccttcta gtaacctgca ggcatgcaag
     7261 cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     7321 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7381 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7441 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7501 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7561 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7621 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7681 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7741 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7801 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7861 gcctccccgc atcgataccg tcgacctcga gggaattaat tcgagctcgg tacctttaag
     7921 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7981 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     8041 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8101 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8161 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     8221 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     8281 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     8341 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     8401 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     8461 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     8521 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     8581 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     8641 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     8701 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     8761 cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     8821 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     8881 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     8941 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     9001 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9061 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9121 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9181 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9241 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9301 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9361 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     9421 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     9481 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     9541 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     9601 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9661 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     9721 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9781 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9841 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9901 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9961 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    10021 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10081 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10141 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10201 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10261 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    10321 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    10381 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    10441 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    10501 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    10561 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    10621 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10681 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10741 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10801 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    10861 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    10921 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaac
//
