LOCUS       sequence-235537-       10435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  neutrophil specific expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, 
            Umulis DM, Wan J, Deng Q
  TITLE     Phenotypical microRNA screen reveals a noncanonical role of CDK2 in 
            regulating neutrophil migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116.
  PUBMED    31451657
REFERENCE   2  (bases 1 to 10435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116."
COMMENT     Sequence Label: Tol2-lyzC-mcherry-2a-CDK2-D145N
FEATURES             Location/Qualifiers
     source          1..10435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    313..333
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2738..2762
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     2764..2780
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2790..2808
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2864..2873
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2870..3577
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3015..3033)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3283..3302
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3587..3643
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(4556..4572)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4603..4621)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(4650..4674)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4679..4813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4703..4722)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4757..4776
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6954..7141,7142..7153)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7187..7203)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7240..7258)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7279..7295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7279..7295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7292..7314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7303..7319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7327..7333,7334..7351,7352..7357))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7372..7393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7510..7527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7681..8269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7761..7780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8440..9231,9232..9300))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9063..9082
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9301..9405)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9431..9886)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9568..9589)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9780..9799
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10013..10035
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10028..10044
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10054..10072
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(10202..10213,10214..10376)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaaggtttt atcctgcttt agaactgttt gtatttaatt atcaaactat aagacagaca
       61 atctaatgcc agtacacgct actcaaagtt gtaaaacctc agatttaact tcagtagaag
      121 ctgattctca aaattgttag tgtcaagcct agctcttttg gggctgaaaa gcaatcctgc
      181 agtgctgaaa agcctctcac aggcagccga tgcgggaaga ggtgtattag tcttgataga
      241 gaggctgcaa atagcaggaa acgtgagcag agactccctg gtgtctgaaa cacaggccag
      301 atgggccgat atcaactttg tatagaaaag ttggctccga attcgccctt aaaactcgag
      361 agtttgactt gggttgatta tagcaaatag tggaacacaa acagattttg aaatattctt
      421 gcaaatttat ttattaaatc cattcaaata ttgtcatttt ttgtactgtg taatttatat
      481 tgatttgctc atacaatatt tacatttcgc ttaaacaata cactattatt tatatttaaa
      541 aatagctata tataaatcaa atgtatagct cttttttaca caattaatca aagctaaaat
      601 atttgtcaca caaatacagt aagccacttc acaatgatat cttcaaaatc tgtgcttgac
      661 tgaatcattt catttattgt tttgtaattc acattatacc ttacgttctc caggcacagt
      721 ttgaattgtt gataaatgaa gataatacat ctgatttaat tagtctcctt tgtttataat
      781 aactttaatc taatttgata gtaataattc cagcaataca tccattacaa taaaaatcgc
      841 ttttttattt tgcacattag tttgccggta tttgtttaaa gtttcaccat acttttaaat
      901 catgtgctat ttgaaaaggg ctgttatgac tggaattaaa tgtttttttt tatatcagtg
      961 ttttagattg aagtacaata tttataggta aaattcacca gcatatgctt aaagatagcc
     1021 atgctccagg tccttgtgtc cttctaaggt ctgtttgtgg tttaagctgt ttgttgacac
     1081 acgcttgtct tctccatttt tctgtgaagt tattgtcagt ttattgcaca ctgggttgca
     1141 ttaagctgca gttttttcac aacagttttc cacattgtaa cagtcagtct ccaaattatt
     1201 ccatcagtcc tcaaatttag tcatttgagg ccatgaaaac acttaaaact acatgaaggt
     1261 agtttggcat taggttttag acttgttctt gtttggcata ttgcaacctg tgtaaaaatg
     1321 atttgatttt tanttttttt tttttttttt ttcagaaaga aacggtccaa aaagagttat
     1381 gtgaacattt atccatgtgt ttaatgtttt ttaaattaaa aaaaaacatt tttattaaac
     1441 cacttgtgac taaacctttt ttagttattc tattttgtga cgagtctgaa tggccaaagt
     1501 actcttgaat ccaaaatctt tatagtaaaa tgtttaatct tacttgcagt gttgggtata
     1561 atttgtaaca aagttgaatt ccatatctgc tgagtgaaaa agtaaagtaa gagattgctg
     1621 taaatattta ggttatttat ttattaatac aatatagaaa gtaacaaaac acaatgtaaa
     1681 aaatcaataa agaaagtaaa tattaaaagt aatgataaac taatgatgta aacaatatta
     1741 gcaacaaagt ttgtacaatt cagggagggt aaagaatttc acctaatagt caaactctgt
     1801 attaaataat ataaaatctt tgttcttagt ctgaagagga gactgtgaga gaaagcttac
     1861 tggttgttaa attagaaaat ttgtaacttg cagattaaaa tgatttaaat aattttataa
     1921 aggccatgtt tgaaacaaat gtaaaaacat aataaatgtt aagaatttca gatattgaga
     1981 ttttacaatt taaaaaaagc aataaagtaa tagcagagac ctacactctt aaagtaaatt
     2041 acccaactct gcttactagt aacttttgga atagttacta gtatccattg aacaaatact
     2101 agaatctaat taaattgtgt gagtgtccaa tgaaaagatt cttgtattta aaacagtacc
     2161 agtatctata tatgtacaaa atttagtgct agcaaaatgc aaataaatac tactgcatat
     2221 aatggattgc attgtaaatc gactcgccat aatacattta tttcctattt ttacttttaa
     2281 caaaaaataa gttcactgag tatttcatgt cattaaatcg cattaaacct cttgcatgct
     2341 ttatgtacac tagtgcagtt cgctaaatga ccaccggtgt cactaataac acgatcgttt
     2401 ctgaattctg cattaaacgc catttatacg ttgctcttat tagtaaacaa tttctccatc
     2461 atgagctcga ccagtattgc atatttcatc ctcttaaatc cagagttgtt tcttttaatc
     2521 actttctctt catgataaaa gcagtttttg gttttcagaa gtttacaaca aacccagtca
     2581 gttattgcct cagcaaacac tggagcaact gcacacttgt gaaaagcttc ttgcgaaaca
     2641 caaacctttc tctggactta taaataaagg cattcagcag ctccaccata cacctccact
     2701 ggtcttgata aagggatcct ttaagggcga attcgaccca agtttgtaca aaaaagcagg
     2761 cttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
     2821 ccgggccccc cctcgaggtc gactcagcag tgatacaggt accgccacca tggtgagcaa
     2881 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     2941 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     3001 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     3061 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     3121 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     3181 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     3241 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     3301 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     3361 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     3421 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     3481 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     3541 ccgccactcc accggcggca tggacgagct gtacaaggga tctggagcca cgaacttctc
     3601 tctgttaaag caagcaggag acgtggaaga aaaccccggt cctatggagt cttttcagaa
     3661 agtggagaag atcggagagg gaacatacgg agtggtttat aaagccaaaa ataaagttac
     3721 aggagaaact gtcgcgctga agaaaatccg attagatacg gaaactgaag gtgttcccag
     3781 cactgccatt cgtgagatct ctctgctaaa agagctcaat caccctaaca tagtcaagtt
     3841 gcgtgatgtg atacacactg aaaataaact ttacttggtg tttgagtttc ttcaccaaga
     3901 cctgaagagg tttatggact cgacgtctgt cagtggcata tccttgccac tcgtgaagag
     3961 ttacctattc cagctgctcc agggattggc cttctgtcac tcccatcgtg ttcttcacag
     4021 agatcttaaa ccccagaatc tcctcatcaa cgctcagggc gagatcaaac tggctaactt
     4081 tggtttggcc agagcgttcg gtgtacctgt gcggacttat acacatgagg ttgtaacttt
     4141 gtggtacaga gctccagaga ttctcctggg atgtaaatat tattctacag ctgttgacat
     4201 ctggagtttg ggctgcatct ttgctgaaat gattactcgg agggcactgt ttcctggaga
     4261 ctctgaaata gatcagctct tccggatatt tcgaactctg ggcactcctg atgagtctat
     4321 atggcctgga gttacatcaa tgccagacta caaaccctcc tttcccaaat gggctcgaca
     4381 ggacctgtct aaagtggtgc ctccccttga tgaagatggc agagaccttc tcgggcaaat
     4441 gttgacctat gatccaaaca agaggatctc ggcaaagaac gctcttgttc atcggttttt
     4501 ccgcgacgtc accatgccag tgcctccttt acgcctataa tcataatcag ccatggatcc
     4561 actagttcta gagcggccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
     4621 tgcgcgcttg gcgtaatcat ggtcatagca cccagctttc ttgtacaaag ttgatgtaga
     4681 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     4741 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     4801 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     4861 gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggccctcg agcatatgag
     4921 atctgtcccg atctttctct tctgtgctgt caatttagag tagtttttga ggtaatttgg
     4981 gtgcattctc tgtaaaaaca acaaataaat atttatattg tatgcaataa atgtaacaat
     5041 tacacataca ctctctgatt acagcattaa agggttagtt cacccaaaaa tgaaaagatg
     5101 tcattaatga ctcaccctca tgtcgttcca agcccgtaac acctccgttc atcttcggaa
     5161 cacagtttaa gatattttag atttagtccg agagctttct gtccctccac tgaaaatgta
     5221 tgtacggtat actgtccatg tccagaaagg taataaaaac atcaaagtag tccatgtgac
     5281 atcagtgggt taggtagaat tatttgaagc atcgaaaata cattttggtc caaaaataac
     5341 aaaacctacg actttattca gcattgtagc gtcactgcga agtcgtgaac gcggattgac
     5401 aacagacccg gaagagaata caatgccgaa taaagtcgta ggttttgtta tttttggacc
     5461 aaaatgtatt cgatgcttca aaaaattcta actaacccac tgatgtcaca tggactactt
     5521 tgatgttttt attacctttc tggacatgga cagtataccg tacatacatt ctcaatggag
     5581 gcacagaaag ctctcggact aaatctaaaa tatcttaaac tgtgttctga agatgaacgg
     5641 aggtcttacg ggcttggaac gacatgaggg cgagtcatta atgacattat tttcattttt
     5701 gggtgaacta accctttaat aaaactgaca tttgcagcca attgcacact ttataatgtt
     5761 aaatacttgt tgattttaga aatacagtag catttagtga caatcaaaca ttaattttat
     5821 ttctgattat attgacaaaa acacaggact gtaaggctga catgacaggg tgaattgatt
     5881 tcattacatt agttttgatg gtaacacttt tacaataagg ttcgttagtt aactacatta
     5941 gttaacatga actaataatg aactgcacgt atacatgtta atttcaacat ttactaatac
     6001 tttatatctt gttaacatta gttaatgcac tgtgaactaa catgaacaaa caatgaacat
     6061 ctgtatttct attaactaac gttaacaaag attaacagat acagtaacaa atgtattgct
     6121 catggttatt taatgttagt taatacatga actaatcaac ctcattgtaa agtgttaccg
     6181 ttttgacaat acaacatgtt tggaaaaaaa tttctcgctt ggttttcttg tgttttgcat
     6241 gaattcaaac acaatgagag cacggaatga taatgattat tcaaagctgc aataggcgcc
     6301 aaatttcccg tggacctaac gttaccaatt acaaatgcat tttagcattt tattacgtga
     6361 gccttatatt gtcaatcgca tgcattagtg aaaataggcc tactacacat ataaaaggcg
     6421 cgccatcaac gtcaaaacat gcacacccaa aaaaaaaaaa aaaaaaaaaa aaaaanacta
     6481 tcagtaaaac aaaatactta atgtacttac ctcaatatgc ttccttaggt ttgatggcga
     6541 acttttgaag gccgatattt ctttattaag cgggagacag aggacacatt tcatcttgaa
     6601 tgaatcttta tttacaccac ttaaagaaaa gaattcgcga agatacggcc acgggtgctc
     6661 ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc
     6721 ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg
     6781 tgcattgtgg taatagatgt gacatgacgt cacttccaaa ggaccaatga acatgtctga
     6841 ccaatttcat ataatgtgaa aacgattttc ataggcagaa taaataacat ttaaattaaa
     6901 ctgggcatca gcgcaattca attggtttgg taatagcaag ggaaaataga atgaagtgat
     6961 ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt
     7021 ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt
     7081 aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta
     7141 ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc cactagttct
     7201 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     7261 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7321 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7381 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7441 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7561 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7621 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7681 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7741 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7801 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7861 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7921 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7981 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8041 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8101 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8161 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8221 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8281 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8341 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8401 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8461 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8521 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8581 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8641 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8701 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8761 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8821 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8881 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8941 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9001 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9061 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9121 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9181 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9241 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9301 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9361 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9421 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     9481 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     9541 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     9601 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     9661 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     9721 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     9781 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     9841 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     9901 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     9961 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    10021 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
    10081 ggtacaggtc attgcacaac accagaaatg ccctctgatc tgcaaaagac gtgaatatct
    10141 gttcagacac ccatatccac tctgttccac acaggtcaga ggtttgtcca ggagttcttg
    10201 acagaggtgt aaaaagtact caaaaatttt actcaagtga aagtacaagt acttagggaa
    10261 aattttactc aattaaaagt aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa
    10321 aagtactcca ttaaaattgt acttgagtat taaggaagta aaagtaaaag caagaaagaa
    10381 aactagagat tcttgtttaa gcttttaatc tcaaaaaaca ttaaatgaaa tgcat
//
