LOCUS       sequence-23563-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  TITLE     Cecilia Lo plasmids
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV3XFLAGm43CT
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          148..244
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             315..380
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ttagcagagc tcgttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
       61 cctccataga agacaccggg accgatccag cctccggact ctagaggatc cggtactcga
      121 ggaactgaaa aaccagaaag ttaactggta agtttagtct ttttgtcttt tatttcaggt
      181 cccggatccg gtggtggtgc aaatcaaaga actgctcctc agtggatgtt gcctttactt
      241 ctaggcctgt acggaagtgt tacttctgct ctaaaagctg cggaattgta cccgcggccg
      301 caattcccgg gatggactac aaagaccatg acggtgatta taaagatcat gacatcgatt
      361 acaaggatga cgatgacaag cttgttaagg atcgcgtgaa gggaagaagc gatccttacc
      421 acgccaccac cggcccactg agcccatcca aagactgcgg atctccaaaa tatgcttact
      481 tcaatggctg ctcctcacca acggccccac tctcacctat gtctcctcct gggtacaagc
      541 tggtcactgg tgacagaaac aattcctcct gccgcaatta caacaagcaa gccagcgagc
      601 aaaactgggc gaattacagc gcagagcaaa atcgaatggg gcaggccgga agcaccatct
      661 ccaactccca cgcccagccg tttgatttcc ctgacgacag ccaaatgcan gaaagttgtg
      721 gtgnacaaan ctcnaccntt antntnggga tcacgacttc canaaancag cancgcncag
      781 canaaactcg nctgagactg gaattaacng ctgnatctcg atgacgattg gtngntntga
      841 naatcgctca attngggcgg gga
//
