LOCUS       sequence-235745-        1602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-Myc aadA-GFP
FEATURES             Location/Qualifiers
     source          1..1602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..60
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             67..849
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="EAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSD
                     IDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRE
                     LQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEA
                     LNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVI
                     LEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     complement(916..944)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1539..1559
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atggcaagca tgactggtgg acaggctagc gaacaaaaac tcatttctga agaggacttg
       61 cctagcgaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
      121 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
      181 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
      241 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
      301 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
      361 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
      421 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
      481 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
      541 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
      601 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
      661 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
      721 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
      781 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
      841 gtgggcaaag aacttgcagt tgaaggaaaa ttggaggtcg ccatggctag taaaggagaa
      901 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      961 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     1021 tttatttgca ctactggaaa actacctgtt ccttggccaa cacttgtcac tactttctct
     1081 tatggtgttc aatgcttttc aagataccca gatcatatga agcggcacga cttcttcaag
     1141 agcgccatgc ctgagggata cgtgcaggag aggaccatct ctttcaagga cgacgggaac
     1201 tacaagacac gtgctgaagt caagtttgag ggagacaccc tcgtcaacag gatcgagctt
     1261 aagggaatcg atttcaagga ggacggaaac atcctcggcc acaagttgga atacaactac
     1321 aactcccaca acgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc
     1381 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     1441 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     1501 gccctttcga aagatcccaa cgaaaagaga gatcacatgg tccttcttga gtttgtaaca
     1561 gctgctggga ttacacatgg catggatgaa ctatacaaat aa
//
