LOCUS       sequence-235747-        1563 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-FLARE16-S aadA-GFP
FEATURES             Location/Qualifiers
     source          1..1563
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..795
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="EAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSD
                     IDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRE
                     LQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEA
                     LNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVI
                     LEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     complement(877..905)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1500..1520
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgggggcta gcgaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
       61 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
      121 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
      181 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
      241 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
      301 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
      361 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
      421 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
      481 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
      541 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
      601 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
      661 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
      721 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
      781 aaggtagtgg gcaaagaact tgttgaagga aaattggagc tagtagaagg tcttaaagtc
      841 gccatggcta gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      901 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      961 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccttggcca
     1021 acacttgtca ctactttctc ttatggtgtt caatgctttt caagataccc agatcatatg
     1081 aagcggcacg acttcttcaa gagcgccatg cctgagggat acgtgcagga gaggaccatc
     1141 tctttcaagg acgacgggaa ctacaagaca cgtgctgaag tcaagtttga gggagacacc
     1201 ctcgtcaaca ggatcgagct taagggaatc gatttcaagg aggacggaaa catcctcggc
     1261 cacaagttgg aatacaacta caactcccac aacgtataca tcacggcaga caaacaaaag
     1321 aatggaatca aagctaactt caaaattaga cacaacattg aagatggaag cgttcaacta
     1381 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     1441 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agatcacatg
     1501 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     1561 taa
//
