LOCUS       sequence-235757-        1122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-Lux-E codon opt. Nt chloroplasts
FEATURES             Location/Qualifiers
     source          1..1122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             612..620
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgtctactt tattaaatat tgatgctact gaaattaaag tatctactga aattgatgat
       61 attattttta cttcttctcc tttaacttta ttatttgaag atcaagaaaa aattcaaaaa
      121 gaattaattt tagaatcttt tcattatcat tataatcata ataaagatta taaatattat
      181 tgtaatattc aaggagtaga tgaaaatatt caatctattg atgatattcc tgtatttcct
      241 acttctatgt ttaaatattc tagattacat actgctgatg aatctaatat tgaaaattgg
      301 tttacttctt ctggaactaa aggagtaaaa tctcatattg ctagagatag acaatctatt
      361 gaaagattat taggatctgt aaattatgga atgaaatatt taggagaatt tcatgaacat
      421 caattagaat tagtaaatat gggacctgat agattttctg cttctaatgt atggtttaaa
      481 tatgtaatgt ctttagtaca attattatat cctactactt ttactgtaga aaatgatgaa
      541 attgattttg aacaaactat tttagcttta aaagctattc aaagaaaagg aaaaggaatt
      601 tgtttaattg gacctcctta ttttatttat ttattatgtc attatatgaa agaacataat
      661 attgaattta atgctggagc tcatatgttt attattactg gaggaggatg gaaaactaaa
      721 caaaaagaag ctttaaatag acaagatttt aatcaattat taatggaaac tttttcttta
      781 tttcatgaat ctcaaattag agatattttt aatcaagtag aattaaatac ttgttttttt
      841 gaagattctt tacaaagaaa acatgtacct ccttgggtat atgctagagc tttagatcct
      901 gtaactttaa ctcctgtaga agatggacaa gaaggattaa tgtcttatat ggatgcttct
      961 tctacttctt atcctacttt tattgtaact gatgatattg gaattgtaag acatttaaaa
     1021 gaacctgatc cttttcaagg aactactgta gaaattgtaa gaagattaaa tactagagaa
     1081 caaaaaggat gttctttatc tatggctact tctttaaaat aa
//
