LOCUS       sequence-23581-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Lee S, Tsai YC, Mattera R, Smith WJ, Kostelansky MS, Weissman AM, 
            Bonifacino JS, Hurley JH
  TITLE     Structural basis for ubiquitin recognition and autoubiquitination by
            Rabex-5.
  JOURNAL   Nat Struct Mol Biol. 2006 Mar . 13(3):264-71.
  PUBMED    16462746
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2006 Mar . 13(3):264-71."
COMMENT     Sequence Label: pGST2-Rabex5 (9-73)
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..42
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(355..377)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(522..540)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        608..712
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcnatctcaa atcgatcgac cgaaaacctg tattttcagg gcgccatggg atccggaatt
       61 catgtggatc agtcggagct cctatgcaag aaaggatgtg gttactacgg caaccctgct
      121 tggcagggtt tctgctccaa gtgctggaga gaggagtacc acaaagccag gcagaagcag
      181 attcaggagg actgggaact ggcagagcga ctgcagcggg aggaagaaga ggcctttgcc
      241 agcagtcagt aaactagtcg cggccgcttt cgaatctaga gcctgcagtc tcgagcggcc
      301 gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
      361 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
      421 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
      481 acgtagcgat agcggagtgt ataattcttg aagacgaaag ggcctcgtga tacgcctatt
      541 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
      601 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      661 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      721 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      781 tcacccagaa acgctggtga aanaaaaant ctaaaaaaca ttnggngcna aannggttta
      841 ctcaantgna ttcacagcgt aaaatct
//
