LOCUS       sequence-235819-        9263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses E. coli codon optimized Cas12c1 effector in the pET28a 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Yan WX, Hunnewell P, Alfonse LE, Carte JM, Keston-Smith E, 
            Sothiselvam S, Garrity AJ, Chong S, Makarova KS, Koonin EV, Cheng 
            DR, Scott DA
  TITLE     Functionally diverse type V CRISPR-Cas systems.
  JOURNAL   Science. 2019 Jan 4;363(6422):88-91. doi: 10.1126/science.aav7271. 
            Epub 2018 Dec 6.
  PUBMED    30523077
REFERENCE   2  (bases 1 to 9263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aav7271"; journalName: "Science"; date: "2019-01-4-
            4"; volume: "363"; issue: "6422"; pages: "88-91"
COMMENT     Sequence Label: pET28a-mH6-Cas12c1
FEATURES             Location/Qualifiers
     source          1..9263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2016..3098)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3060..3079
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3099..3176)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3382..3401
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     3485..3504
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3485..3503
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3504..3528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(3537..3542,3543..3560,3561..3566)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             3586..3608
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3640..3657
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7682..7700)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7696..7743
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7780..8235
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7867..7886)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8077..8098
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8327..9142)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8327..9142)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9050..9069
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9134..9153
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagattta
     3541 cactttatgc ttccggctcg tatgtttcta gaaagcttaa ataattttgt ttaactttaa
     3601 gaaggagatt taaatatgaa aatcgaagaa ggtaaaggtc accatcacca tcaccacatg
     3661 cagaccaaga aaacccacct gcatctgatc agcgcgaagg cgagccgtaa ataccgtcgt
     3721 accattgcgt gcctgagcga caccgcgaag aaagatctgg agcgtcgtaa acagagcggt
     3781 gcggcggacc cggcgcaaga actgagctgc ctgaagacca tcaagttcaa gctggaagtg
     3841 ccggaaggta gcaaactgcc gagctttgat cgtatcagcc aaatttacaa cgcgctggaa
     3901 accattgaaa aaggcagcct gagctatctg ctgttcgcgc tgattctgag cggtttccgt
     3961 atctttccga acagcagcgc ggcgaagacc ttcgcgagca gcagctgcta caaaaacgac
     4021 cagtttgcga gccaaatcaa ggagattttc ggcgaaatgg ttaaaaactt tatcccgagc
     4081 gagctggaaa gcattctgaa gaaaggtcgt cgtaagaaca acaaagattg gaccgaggaa
     4141 aacatcaagc gtgtgctgaa cagcgagttc ggccgtaaaa acagcgaagg tagcagcgcg
     4201 ctgttcgaca gctttctgag caagtttagc caggagctgt tccgtaaatt tgatagctgg
     4261 aacgaagtta acaagaaata cctggaggcg gcggaactgc tggacagcat gctggcgagc
     4321 tatggcccgt tcgatagcgt gtgcaagatg atcggtgaca gcgatagccg taacagcctg
     4381 ccggacaaaa gcaccattgc gtttaccaac aacgcggaga tcaccgttga tattgaaagc
     4441 agcgtgatgc cgtacatggc gattgcggcg ctgctgcgtg agtatcgtca gagcaagagc
     4501 aaagcggcgc cggtggcgta cgttcaaagc cacctgacca ccaccaacgg caacggtctg
     4561 agctggttct ttaagttcgg tctggacctg atccgtaaag cgccggttag cagcaagcag
     4621 agcaccagcg atggcagcaa gagcctgcaa gagctgttca gcgtgccgga cgataaactg
     4681 gacggtctga agtttattaa agaggcgtgc gaagcgctgc cggaagcgag cctgctgtgc
     4741 ggcgagaaag gtgaactgct gggctaccag gacttccgta ccagctttgc gggtcatatc
     4801 gatagctggg tggcgaacta tgttaaccgt ctgttcgagc tgattgaact ggtgaaccaa
     4861 ctgccggaga gcattaagct gccgagcatc ctgacccaga aaaaccacaa cctggttgcg
     4921 agcctgggtc tgcaagaggc ggaagtgagc catagcctgg agctgttcga aggcctggtg
     4981 aagaacgttc gtcagaccct gaagaaactg gcgggtatcg atatcagcag cagcccgaac
     5041 gagcaagaca tcaaagagtt ctacgcgttt agcgatgttc tgaaccgtct gggcagcatc
     5101 cgtaaccaga ttgagaacgc ggtgcaaacc gcgaagaaag acaagattga tctggaaagc
     5161 gcgatcgagt ggaaagaatg gaagaaactg aagaaactgc cgaagctgaa cggtctgggt
     5221 ggcggtgttc cgaaacagca agagctgctg gacaaagcgc tggaaagcgt gaagcagatt
     5281 cgtcactatc aacgtatcga tttcgagcgt gttattcagt gggcggtgaa cgagcattgc
     5341 ctggaaaccg ttccgaagtt tctggtggac gcggagaaga aaaagatcaa caaggaaagc
     5401 agcaccgatt tcgcggcgaa agagaacgcg gttcgttttc tgctggaagg tattggtgcg
     5461 gcggcgcgtg gcaaaaccga cagcgtgagc aaggcggcgt acaactggtt cgtggttaac
     5521 aactttctgg cgaaaaagga tctgaaccgt tatttcatca actgccaagg ttgcatttac
     5581 aaaccgccgt atagcaagcg tcgtagcctg gcgtttgcgc tgcgtagcga caacaaggat
     5641 accatcgagg tggtttggga gaaattcgaa accttttaca aggaaatcag caaggagatc
     5701 gaaaagttca acatttttag ccaggagttc caaacctttc tgcacctgga aaacctgcgt
     5761 atgaagctgc tgctgcgtcg tatccagaaa ccgattccgg cggagatcgc gttctttagc
     5821 ctgccgcaag aatactatga cagcctgccg ccgaacgttg cgttcctggc gctgaaccag
     5881 gagatcaccc cgagcgaata cattacccaa ttcaacctgt atagcagctt tctgaacggc
     5941 aacctgatcc tgctgcgtcg tagccgtagc tatctgcgtg cgaagttcag ctgggtgggt
     6001 aacagcaaac tgatctacgc ggcgaaagag gcgcgtctgt ggaaaattcc gaacgcgtat
     6061 tggaaaagcg acgaatggaa gatgatcctg gatagcaacg tgctggtttt cgacaaagcg
     6121 ggtaacgttc tgccggcgcc gaccctgaaa aaggtgtgcg agcgtgaagg tgacctgcgt
     6181 ctgttctacc cgctgctgcg tcagctgccg catgattggt gctatcgtaa cccgtttgtg
     6241 aagagcgttg gccgtgagaa aaacgtgatc gaagttaaca aagagggtga gccgaaagtt
     6301 gcgagcgcgc tgccgggcag cctgttccgt ctgattggtc cggcgccgtt taagagcctg
     6361 ctggacgatt gcttctttaa cccgctggac aaagatctgc gtgagtgcat gctgattgtt
     6421 gaccaggaga tcagccaaaa agttgaagcg cagaaggtgg aggcgagcct ggaaagctgc
     6481 acctacagca ttgcggtgcc gatccgttat cacctggagg aaccgaaggt tagcaaccag
     6541 ttcgagaacg tgctggcgat cgatcaaggc gaagcgggtc tggcgtacgc ggtgtttagc
     6601 ctgaagagca tcggcgaggc ggaaaccaaa ccgattgcgg ttggcaccat ccgtattccg
     6661 agcattcgtc gtctgatcca tagcgtgagc acctaccgta aaaagaaaca gcgtctgcaa
     6721 aacttcaaac agaactatga cagcaccgcg tttatcatgc gtgagaacgt gaccggcgat
     6781 gtttgcgcga agattgttgg tctgatgaaa gagttcaacg cgtttccggt tctggagtac
     6841 gacgtgaaga acctggaaag cggtagccgt cagctgagcg cggtgtacaa agcggttaac
     6901 agccacttcc tgtattttaa ggagccgggc cgtgacgcgc tgcgtaaaca actgtggtat
     6961 ggcggtgaca gctggaccat cgatggtatc gaaattgtga cccgtgagcg taaagaagat
     7021 ggcaaagagg gtgtggaaaa aattgttccg ctgaaagtgt tcccgggccg tagcgttagc
     7081 gcgcgtttta ccagcaaaac ctgcagctgc tgcggtcgta acgtgttcga ctggctgttt
     7141 accgagaaga aagcgaagac caacaagaaa ttcaacgtta acagcaaagg cgagctgacc
     7201 accgcggacg gtgtgatcca gctgttcgaa gcggatcgta gcaagggccc gaaattttac
     7261 gcgcgtcgta aggagcgtac cccgctgacc aaaccgatcg cgaagggtag ctatagcctg
     7321 gaggaaattg aacgtcgtgt tcgtaccaac ctgcgtcgtg cgccgaagag caaacagagc
     7381 cgtgacacca gccagagcca atacttctgc gtgtataaag actgcgcgct gcactttagc
     7441 ggcatgcaag cggatgaaaa cgcggcgatc aacattggtc gtcgtttcct gaccgcgctg
     7501 cgtaagaacc gtcgtagcga ttttccgagc aacgttaaaa tcagcgaccg tctgctggat
     7561 aactaataaa agcttgaatt cctcgagaga gaccatggcc accggtctct atcgaagata
     7621 tgaggagctc aaactcgagc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     7681 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     7741 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
     7801 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7861 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7921 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7981 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8041 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8101 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8161 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     8221 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8281 atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat
     8341 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     8401 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     8461 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     8521 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     8581 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     8641 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     8701 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     8761 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     8821 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     8881 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     8941 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     9001 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     9061 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg
     9121 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     9181 ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9241 gtagaaaaga tcaaaggatc ttc
//
