LOCUS       sequence-23582-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Lee S, Tsai YC, Mattera R, Smith WJ, Kostelansky MS, Weissman AM, 
            Bonifacino JS, Hurley JH
  TITLE     Structural basis for ubiquitin recognition and autoubiquitination by
            Rabex-5.
  JOURNAL   Nat Struct Mol Biol. 2006 Mar . 13(3):264-71.
  PUBMED    16462746
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2006 Mar . 13(3):264-71."
COMMENT     Sequence Label: pGST2-Ub CPS (8-16)
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..41
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             54..281
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(442..464)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(609..627)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gnatctcaaa tcgatcgacc gaaaacctgt attttcaggg cgccatggga tccatgcaaa
       61 tttttgtcaa gactttgact ggtaagacca tcactttgga agttgaatct tctgacacta
      121 ttgacaatgt caagtcaaag attcaagaca aggaaggtat cccacctgac caacaaagat
      181 tgatctttgc tggtaagcaa ttggaagacg gtagaacctt gtctgactac aacattcaaa
      241 aagaatccac tttgcactta gtcttgagat tgagaggtgg taaagcgccg cgcaaaagcc
      301 tgtggtgttg agaattcaaa ggcctacgtc gacgagctca ctagtcgcgg ccgctttcga
      361 atctagagcc tgcagtctcg agcggccgca tcgtgactga ctgacgatct gcctcgcgcg
      421 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
      481 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
      541 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag
      601 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      661 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      721 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataat gttccttatt
      781 ttgaaaagtn ggatttgatt tccactttcc gnncccctnt nccttttgng natttgcctc
      841 tgtttgcccc naanc
//
