LOCUS       sequence-235820-        9555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression in cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, 
            Umulis DM, Wan J, Deng Q
  TITLE     Phenotypical microRNA screen reveals a noncanonical role of CDK2 in 
            regulating neutrophil migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116.
  PUBMED    31451657
REFERENCE   2  (bases 1 to 9555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116."
COMMENT     Sequence Label: hsa-miR199 plix 4.03
FEATURES             Location/Qualifiers
     source          1..9555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        34..544
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     463..481
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             554..1150
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(554..573)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1050..1070
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1151..1204
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1205..1822,1823..1828,1829..1864,1865..1867,1868..1903,
                     1904..1906,1907..1942,1943..1951)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(1991..2011)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2409..2420)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2625..2858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2930..3051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2967..2986)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3021..3040
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3091..3226
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3226..3297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3298..3366,3367..4158)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3516..3535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4329..4917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4818..4837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5071..5088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5205..5226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5241..5246,5247..5264,5265..5271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5279..5295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5284..5306
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5303..5319
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5303..5319
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5338..5358
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5340..5358
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5386..5612
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5613..5793
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5840..5965
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6458..6691
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6876..6920
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7133..7250
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7304..7618
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    7312..7330
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7348..7366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7383..7401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7419..7437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7455..7473
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7490..7508
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7526..7544
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     7591..7615
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      7635..7644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8863..9555
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 ccggtccacc accaccacca ccactaagga tccggggttg gggttgcgcc ttttccaagg
       61 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
      121 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
      181 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
      241 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
      301 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
      361 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
      421 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
      481 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
      541 ccagcaattc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
      601 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
      661 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
      721 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
      781 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
      841 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
      901 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
      961 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     1021 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     1081 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     1141 gcccggtgcc gaaggtagag gttctctcct cacttgtggt gatgttgaag aaaaccctgg
     1201 tccaatgtct agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg
     1261 tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     1321 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     1381 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     1441 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     1501 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     1561 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     1621 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     1681 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     1741 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     1801 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     1861 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     1921 cgattttgac cttgacatgc tccccgggta actaagtaag gatcgatcca agatatcgta
     1981 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2041 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2101 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2161 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2221 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2281 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     2341 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     2401 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2461 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     2521 ccccgcctgt ttcgcctcgg cgtccggact agaggtacct ttaagaccaa tgacttacaa
     2581 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     2641 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     2701 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     2761 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     2821 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     2881 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     2941 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3001 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3061 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3121 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3181 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctttc aaatatgtat
     3241 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3301 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3361 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3421 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3481 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3541 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3601 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3661 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3721 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3781 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3841 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3901 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3961 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4021 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4081 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4141 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4201 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4261 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4321 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4381 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4441 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     4501 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4561 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4621 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4681 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4741 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4801 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4861 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4921 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4981 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     5041 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     5101 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     5161 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     5221 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     5281 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     5341 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     5401 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     5461 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     5521 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     5581 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     5641 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5701 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5761 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     5821 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     5881 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     5941 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     6001 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     6061 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     6121 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     6181 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     6241 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     6301 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     6361 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     6421 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     6481 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     6541 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     6601 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     6661 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     6721 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     6781 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     6841 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     6901 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     6961 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     7021 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     7081 ccccgagggg acaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa
     7141 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     7201 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     7261 acagggacag cagagatcca ctttggccgc gaatcgatat gtcgagttta ctccctatca
     7321 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt
     7381 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     7441 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt
     7501 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag
     7561 gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     7621 gagaattggc tagcgccacc atggatgagg aaatcgctgc cctggtcgtt gacaacggct
     7681 ccggtatgtg caaagccggt tttgctggag atgatgcccc tcgtgctgtt ttcccctcca
     7741 ttgttggacg acccagacat caggtacatt taataaacca tcctaataac atgttaatca
     7801 tattgaacca cattaaactc tcatttgtgc attttacatc ttttaaaacc gatttatatt
     7861 cgtttttaga taatgaagtt tattaaagtg ttaattcaca gctttattgc ttgttttgag
     7921 aatctgcttg cataagtgat ttttaaactt gatctgattt tcgtttgttt cgaagttctt
     7981 cagctttgaa agtcagatct tcaattcaca gccatgccat agttttagga tacagtataa
     8041 gtagacgaat tgggatgcat cgtagatttt atgctgtgtt tgttatggtt tttcagtggt
     8101 agagattttc agagtattat tagggttata gtcagtgtca ggtttattta tcgtcagcgt
     8161 gatgggcaaa aaacagctgg cagtacgggc tcggggtggt ggaaaatgac actcacctgc
     8221 cggcgagttt ggggggcctg tctcgacgag ccgctcaccc aagcccagcc taaccaatgt
     8281 gcagactact gtacacattg agagcctcct gaacaggtag tctgaacact gggttggcgg
     8341 ggccggctat ccatcctgca gaacccccca ggcagacgct ggggccacca ggccaggccg
     8401 tgcctcagca cctctgagca gccaaggaaa ccaccactct tcccgagacg atccgctctg
     8461 tcccttctga agccaactgc tgttactctc gcccagaaat ctgcgccaac atggacacac
     8521 ccagtggagc aagccatctg attttcatgg tcagattgta aagaagcaat tactcttgga
     8581 cagtttaagc agaaaagcca ccaagtcaag tcactggtca tatgatgaac tcgaagatct
     8641 aataaaagtt aatttaagcg ttcattacaa taaattcaag tgaatctgtt ttaagctagc
     8701 ctttaacgtt tttaaattcc ttcaaacaaa ttagttcctc tttgcagttc ctcatttctc
     8761 ggtgagctgg tgctttaatc cctttacttt ttcttcctct agggagtgat ggttggcatg
     8821 ggacgaatta attcctgcag cccgggggta ccggtcgcca ccatgaacac cccgggaatt
     8881 aacctgatca aggaggacat gcgcgtgaag gtgcacatgg agggcaacgt gaacggccac
     8941 gccttcgtga tcgagggcga gggcaagggc aagccctacg agggcaccca gaccgccaac
     9001 ctgaccgtga aggagggcgc ccccctgccc ttcagctacg acatcctgac caccgccgtg
     9061 cactacggca accgggtgtt caccaagtac cccgaggaca tccccgacta cttcaagcag
     9121 agcttccccg agggctacag ctgggagcgc accatgacct tcgaggacaa gggcatctgc
     9181 accatccgca gcgacatcag cctggagggc gactgcttct tccagaacgt gcgcttcaag
     9241 ggcaccaact tcccccccaa cggccccgtg atgcagaaga aaaccctgaa gtgggagccc
     9301 agcaccgaga agctgcacgt gcgcgacggc ctgctggtgg gcaacatcaa catggccctg
     9361 ctgctggagg gcggcggcca ctacctgtgc gacttcaaga ccacctacaa ggccaagaag
     9421 gtggtgcagc tgcccgacgc ccacttcgtg gaccaccgca tcgagatcct gggcaacgac
     9481 agcgactaca acaaggtgaa gctgtacgag cacgccgtgg cccgctacag ccccctgccc
     9541 agccaggtgt ggtaa
//
