LOCUS       sequence-235822-        9340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression in cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, 
            Umulis DM, Wan J, Deng Q
  TITLE     Phenotypical microRNA screen reveals a noncanonical role of CDK2 in 
            regulating neutrophil migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116.
  PUBMED    31451657
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116."
COMMENT     Sequence Label: vector plix 4.03
FEATURES             Location/Qualifiers
     source          1..9340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    28..145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        199..513
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    207..225
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    243..261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    278..296
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    314..332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    350..368
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    385..403
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    421..439
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     486..510
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      529..538
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1502..2194
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     CDS             2200..2226
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2242..2259
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2269..2779
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2698..2716
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2789..3385
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2789..2808)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3285..3305
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3386..3439
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3440..4057,4058..4063,4064..4099,4100..4102,4103..4138,
                     4139..4141,4142..4177,4178..4186)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4226..4246)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4644..4655)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4860..5093
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5165..5286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5202..5221)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5256..5275
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5326..5461
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5461..5532
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5533..5601,5602..6393)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5751..5770)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6564..7152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7053..7072
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7306..7323
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7440..7461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7476..7481,7482..7499,7500..7506)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7514..7530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7519..7541
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7538..7554
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7538..7554
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7573..7593
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7575..7593
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7621..7847
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7848..8028
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8075..8200
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8693..8926
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9111..9155
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
       61 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
      121 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
      181 gccgcgaatc gatatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      241 actccctatc agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt
      301 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
      361 gatagagaac gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac
      421 tccctatcag tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata
      481 agcagagctc gtttagtgaa ccgtcagatc gcctggagaa ttggctaggc caccatggat
      541 gaggaaatcg ctgccctggt cgttgacaac ggctccggta tgtgcaaagc cggttttgct
      601 ggagatgatg cccctcgtgc tgttttcccc tccattgttg gacgacccag acatcaggta
      661 catttaataa accatcctaa taacatgtta atcatattga accacattaa actctcattt
      721 gtgcatttta catcttttaa aaccgattta tattcgtttt tagataatga agtttattaa
      781 agtgttaatt cacagcttta ttgcttgttt tgagaatctg cttgcataag tgatttttaa
      841 acttgatctg attttcgttt gtttcgaagt tcttcagctt tgaaagtcag atcttcaatt
      901 cacagccatg ccatagtttt aggatacagt ataagtagac gaattgggat gcatcgtaga
      961 ttttatgctg tgtttgttat ggtttttcag tggtagagat tttcagagta ttattagggt
     1021 tatagtcagt gtcaggttta tttatcgtca gcgtgatggg caaaaaacag ctggcagtac
     1081 gggctatgtc ttcgtctaga agaagacaaa gccaactgct gttactctcg cccagaaatc
     1141 tgcgccaaca tggacacacc cagtggagca agccatctga ttttcatggt cagattgtaa
     1201 agaagcaatt actcttggac agtttaagca gaaaagccac caagtcaagt cactggtcat
     1261 atgatgaact cgaagatcta ataaaagtta atttaagcgt tcattacaat aaattcaagt
     1321 gaatctgttt taagctagcc tttaacgttt ttaaattcct tcaaacaaat tagttcctct
     1381 ttgcagttcc tcatttctcg gtgagctggt gctttaatcc ctttactttt tcttcctcta
     1441 gggagtgatg gttggcatgg gacgaattaa ttcctgcagc ccgggggtac cggtcgccac
     1501 catgaacacc ccgggaatta acctgatcaa ggaggacatg cgcgtgaagg tgcacatgga
     1561 gggcaacgtg aacggccacg ccttcgtgat cgagggcgag ggcaagggca agccctacga
     1621 gggcacccag accgccaacc tgaccgtgaa ggagggcgcc cccctgccct tcagctacga
     1681 catcctgacc accgccgtgc actacggcaa ccgggtgttc accaagtacc ccgaggacat
     1741 ccccgactac ttcaagcaga gcttccccga gggctacagc tgggagcgca ccatgacctt
     1801 cgaggacaag ggcatctgca ccatccgcag cgacatcagc ctggagggcg actgcttctt
     1861 ccagaacgtg cgcttcaagg gcaccaactt cccccccaac ggccccgtga tgcagaagaa
     1921 aaccctgaag tgggagccca gcaccgagaa gctgcacgtg cgcgacggcc tgctggtggg
     1981 caacatcaac atggccctgc tgctggaggg cggcggccac tacctgtgcg acttcaagac
     2041 cacctacaag gccaagaagg tggtgcagct gcccgacgcc cacttcgtgg accaccgcat
     2101 cgagatcctg ggcaacgaca gcgactacaa caaggtgaag ctgtacgagc acgccgtggc
     2161 ccgctacagc cccctgccca gccaggtgtg gtaaccggtt atccgtatga tgtgccggat
     2221 tatgcgtaaa cgcgtccggt ccaccaccac caccaccact aaggatccgg ggttggggtt
     2281 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     2341 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     2401 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     2461 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     2521 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     2581 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     2641 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     2701 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     2761 caccgacctc tctccccagc aattcaccat gaccgagtac aagcccacgg tgcgcctcgc
     2821 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     2881 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     2941 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     3001 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     3061 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     3121 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     3181 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     3241 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     3301 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     3361 gtgcatgacc cgcaagcccg gtgccgaagg tagaggttct ctcctcactt gtggtgatgt
     3421 tgaagaaaac cctggtccaa tgtctagact ggacaagagc aaagtcataa acggagctct
     3481 ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     3541 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     3601 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
     3661 atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg
     3721 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     3781 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     3841 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
     3901 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     3961 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     4021 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga
     4081 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
     4141 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa gtaaggatcg
     4201 atccaagata tcgtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4261 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4321 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4381 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4441 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4501 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4561 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4621 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4681 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4741 ccctttgggc cgcctccccg cctgtttcgc ctcggcgtcc ggactagagg tacctttaag
     4801 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4861 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     4921 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4981 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5041 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     5101 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     5161 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5221 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5281 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     5341 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5401 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5461 ctttcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5521 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5581 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5641 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5701 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5761 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5821 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5881 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5941 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6001 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6061 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6121 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6181 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6241 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6301 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6361 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6421 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6481 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6541 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6601 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6661 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6721 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6781 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6841 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6901 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6961 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7021 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7081 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7141 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7201 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7261 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7321 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7381 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7441 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7501 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     7561 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     7621 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     7681 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     7741 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     7801 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     7861 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7921 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7981 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     8041 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     8101 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     8161 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     8221 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     8281 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     8341 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     8401 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     8461 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     8521 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     8581 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     8641 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     8701 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     8761 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     8821 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     8881 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     8941 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     9001 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     9061 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     9121 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     9181 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     9241 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     9301 agacccacct cccaaccccg aggggacaat tctcgacctc
//
