LOCUS       sequence-235824-        9591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression in cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, 
            Umulis DM, Wan J, Deng Q
  TITLE     Phenotypical microRNA screen reveals a noncanonical role of CDK2 in 
            regulating neutrophil migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116.
  PUBMED    31451657
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116."
COMMENT     Sequence Label: hsa-miR-100 plix4.03
FEATURES             Location/Qualifiers
     source          1..9591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    253..370
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        424..738
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    432..450
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    468..486
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    503..521
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    539..557
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    575..593
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    610..628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    646..664
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     711..735
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      754..763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1978..2670
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     CDS             2676..2702
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2718..2735
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2745..3255
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3174..3192
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3265..3861
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3265..3284)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3761..3781
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3862..3915
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3916..4533,4534..4539,4540..4575,4576..4578,4579..4614,
                     4615..4617,4618..4653,4654..4662)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4702..4722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5120..5131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5336..5569
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5641..5762
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5678..5697)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5732..5751
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5802..5937
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5937..6008
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6009..6077,6078..6869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6227..6246)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7040..7628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7529..7548
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7782..7799
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7916..7937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7952..7957,7958..7975,7976..7982)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7990..8006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7995..8017
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8014..8030
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8014..8030
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8049..8069
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8051..8069
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8097..8323
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8324..8504
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8551..8676
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9169..9402
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(9587..9591,1..40)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
       61 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
      121 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
      181 gtttcagacc cacctcccaa ccccgagggg acaattctcg acctcgagac aaatggcagt
      241 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      301 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      361 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc gaatcgatat
      421 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      481 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      541 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      601 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      661 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      721 gtgaaccgtc agatcgcctg gagaattggc taggccacca tggatgagga aatcgctgcc
      781 ctggtcgttg acaacggctc cggtatgtgc aaagccggtt ttgctggaga tgatgcccct
      841 cgtgctgttt tcccctccat tgttggacga cccagacatc aggtacattt aataaaccat
      901 cctaataaca tgttaatcat attgaaccac attaaactct catttgtgca ttttacatct
      961 tttaaaaccg atttatattc gtttttagat aatgaagttt attaaagtgt taattcacag
     1021 ctttattgct tgttttgaga atctgcttgc ataagtgatt tttaaacttg atctgatttt
     1081 cgtttgtttc gaagttcttc agctttgaaa gtcagatctt caattcacag ccatgccata
     1141 gttttaggat acagtataag tagacgaatt gggatgcatc gtagatttta tgctgtgttt
     1201 gttatggttt ttcagtggta gagattttca gagtattatt agggttatag tcagtgtcag
     1261 gtttatttat cgtcagcgtg atgggcaaaa aacagctggc agtacgggct ctctccacag
     1321 tcgtgtgcgg tcctcagggc agtgacctac gctccagcca cctcttgcct ccccctcgcc
     1381 tgcccagtgt tcagactacc tgttcatcat gctgcagctg aacagtagtc cgcacattgg
     1441 ttaggctggg ctgggacaca cacacccgcc aagccgccgt aagatctctg ccattcgccg
     1501 gtgaacgaat aaagcaagac tttagccccg cacaatacac cgtttcagca tcgacatcgc
     1561 aatgaaccac atcgctgaat tcacaagcca actgctgtta ctctcgccca gaaatctgcg
     1621 ccaacatgga cacacccagt ggagcaagcc atctgatttt catggtcaga ttgtaaagaa
     1681 gcaattactc ttggacagtt taagcagaaa agccaccaag tcaagtcact ggtcatatga
     1741 tgaactcgaa gatctaataa aagttaattt aagcgttcat tacaataaat tcaagtgaat
     1801 ctgttttaag ctagccttta acgtttttaa attccttcaa acaaattagt tcctctttgc
     1861 agttcctcat ttctcggtga gctggtgctt taatcccttt actttttctt cctctaggga
     1921 gtgatggttg gcatgggacg aattaattcc tgcagcccgg gggtaccggt cgccaccatg
     1981 aacaccccgg gaattaacct gatcaaggag gacatgcgcg tgaaggtgca catggagggc
     2041 aacgtgaacg gccacgcctt cgtgatcgag ggcgagggca agggcaagcc ctacgagggc
     2101 acccagaccg ccaacctgac cgtgaaggag ggcgcccccc tgcccttcag ctacgacatc
     2161 ctgaccaccg ccgtgcacta cggcaaccgg gtgttcacca agtaccccga ggacatcccc
     2221 gactacttca agcagagctt ccccgagggc tacagctggg agcgcaccat gaccttcgag
     2281 gacaagggca tctgcaccat ccgcagcgac atcagcctgg agggcgactg cttcttccag
     2341 aacgtgcgct tcaagggcac caacttcccc cccaacggcc ccgtgatgca gaagaaaacc
     2401 ctgaagtggg agcccagcac cgagaagctg cacgtgcgcg acggcctgct ggtgggcaac
     2461 atcaacatgg ccctgctgct ggagggcggc ggccactacc tgtgcgactt caagaccacc
     2521 tacaaggcca agaaggtggt gcagctgccc gacgcccact tcgtggacca ccgcatcgag
     2581 atcctgggca acgacagcga ctacaacaag gtgaagctgt acgagcacgc cgtggcccgc
     2641 tacagccccc tgcccagcca ggtgtggtaa ccggttatcc gtatgatgtg ccggattatg
     2701 cgtaaacgcg tccggtccac caccaccacc accactaagg atccggggtt ggggttgcgc
     2761 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     2821 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     2881 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     2941 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     3001 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     3061 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     3121 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     3181 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     3241 gacctctctc cccagcaatt caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     3301 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     3361 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     3421 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     3481 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     3541 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     3601 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     3661 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     3721 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     3781 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     3841 atgacccgca agcccggtgc cgaaggtaga ggttctctcc tcacttgtgg tgatgttgaa
     3901 gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaacgg agctctggaa
     3961 ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
     4021 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
     4081 ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg
     4141 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac
     4201 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
     4261 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
     4321 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
     4381 gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca agcaattgag
     4441 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
     4501 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt
     4561 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
     4621 gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa ggatcgatcc
     4681 aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4741 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4801 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4861 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4921 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4981 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5041 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5101 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5161 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5221 ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca
     5281 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5341 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     5401 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5461 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5521 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     5581 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     5641 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5701 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5761 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     5821 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5881 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt
     5941 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6001 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6061 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6121 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6181 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6241 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6301 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6361 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6421 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6481 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6541 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6601 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6661 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6721 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6781 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6841 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6901 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6961 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7021 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7081 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7141 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7201 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7261 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7321 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7381 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7441 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7501 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7561 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7621 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7681 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7741 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7801 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7861 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7921 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7981 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     8041 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     8101 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     8161 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     8221 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     8281 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     8341 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8401 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8461 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     8521 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     8581 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8641 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     8701 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     8761 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     8821 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     8881 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     8941 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     9001 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     9061 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     9121 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     9181 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     9241 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     9301 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     9361 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     9421 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     9481 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     9541 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga a
//
