LOCUS       sequence-235826-        9559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression in cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, 
            Umulis DM, Wan J, Deng Q
  TITLE     Phenotypical microRNA screen reveals a noncanonical role of CDK2 in 
            regulating neutrophil migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116.
  PUBMED    31451657
REFERENCE   2  (bases 1 to 9559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116."
COMMENT     Sequence Label: hsa-miR 99 plix 4.03
FEATURES             Location/Qualifiers
     source          1..9559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    214..331
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        385..699
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    393..411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    429..447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    464..482
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    500..518
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    536..554
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    571..589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     672..696
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      716..725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1948..2640
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     CDS             2647..2664
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2674..3184
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3103..3121
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3194..3790
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3194..3213)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3690..3710
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3791..3844
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3845..4462,4463..4468,4469..4504,4505..4507,4508..4543,
                     4544..4546,4547..4582,4583..4591)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4631..4651)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5049..5060)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5265..5498
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5570..5691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5607..5626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5661..5680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5731..5866
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5866..5937
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5938..6006,6007..6798)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6156..6175)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6969..7557
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7458..7477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7711..7728
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7845..7866
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7881..7886,7887..7904,7905..7911)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7919..7935
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7924..7946
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7943..7959
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7943..7959
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7978..7998
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7980..7998
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8026..8252
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8253..8433
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8480..8605
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9098..9331
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(9516..9559,1)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
       61 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
      121 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacaattctc
      181 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
      241 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
      301 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
      361 actttggccg cgaatcgata tgtcgagttt actccctatc agtgatagag aacgtatgtc
      421 gagtttactc cctatcagtg atagagaacg atgtcgagtt tactccctat cagtgataga
      481 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      541 atcagtgata gagaacgtat gtcgagttta tccctatcag tgatagagaa cgtatgtcga
      601 gtttactccc tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc
      661 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagaattgg ctagcgccac
      721 catggatgag gaaatcgctg ccctggtcgt tgacaacggc tccggtatgt gcaaagccgg
      781 ttttgctgga gatgatgccc ctcgtgctgt tttcccctcc attgttggac gacccagaca
      841 tcaggtacat ttaataaacc atcctaataa catgttaatc atattgaacc acattaaact
      901 ctcatttgtg cattttacat cttttaaaac cgatttatat tcgtttttag ataatgaagt
      961 ttattaaagt gttaattcac agctttattg cttgttttga gaatctgctt gcataagtga
     1021 tttttaaact tgatctgatt ttcgtttgtt tcgaagttct tcagctttga aagtcagatc
     1081 ttcaattcac agccatgcca tagttttagg atacagtata agtagacgaa ttgggatgca
     1141 tcgtagattt tatgctgtgt ttgttatggt ttttcagtgg tagagatttt cagagtatta
     1201 ttagggttat agtcagtgtc aggtttattt atcgtcagcg tgatgggcaa aaaacagctg
     1261 gcagtacggg cttaataggg ggcccatgca agatgttgcc cattggcata aacccgtaga
     1321 tccgatcttg tggtgaagtg gaccgcacaa gctcgcttct atgggtctgt gtcagtgtgg
     1381 taatctgaca aaatgctata cacagtgccg ttcaacaata gttcagtaaa atcctgttaa
     1441 actccagttg attatatact tttgaagtca ttatattttc ctttgttttt aatgtttatt
     1501 ttaatcattg tctgtcttac aaggcaggct tcaattctca acaacttgga agcgtagcca
     1561 actgctgtta ctctcgccca gaaatctgcg ccaacatgga cacacccagt ggagcaagcc
     1621 atctgatttt catggtcaga ttgtaaagaa gcaattactc ttggacagtt taagcagaaa
     1681 agccaccaag tcaagtcact ggtcatatga tgaactcgaa gatctaataa aagttaattt
     1741 aagcgttcat tacaataaat tcaagtgaat ctgttttaag ctagccttta acgtttttaa
     1801 attccttcaa acaaattagt tcctctttgc agttcctcat ttctcggtga gctggtgctt
     1861 taatcccttt actttttctt cctctaggga gtgatggttg gcatgggacg aattaattcc
     1921 tgcagcccgg gggtaccggt cgccaccatg aacaccccgg gaattaacct gatcaaggag
     1981 gacatgcgcg tgaaggtgca catggagggc aacgtgaacg gccacgcctt cgtgatcgag
     2041 ggcgagggca agggcaagcc ctacgagggc acccagaccg ccaacctgac cgtgaaggag
     2101 ggcgcccccc tgcccttcag ctacgacatc ctgaccaccg ccgtgcacta cggcaaccgg
     2161 gtgttcacca agtaccccga ggacatcccc gactacttca agcagagctt ccccgagggc
     2221 tacagctggg agcgcaccat gaccttcgag gacaagggca tctgcaccat ccgcagcgac
     2281 atcagcctgg agggcgactg cttcttccag aacgtgcgct tcaagggcac caacttcccc
     2341 cccaacggcc ccgtgatgca gaagaaaacc ctgaagtggg agcccagcac cgagaagctg
     2401 cacgtgcgcg acggcctgct ggtgggcaac atcaacatgg ccctgctgct ggagggcggc
     2461 ggccactacc tgtgcgactt caagaccacc tacaaggcca agaaggtggt gcagctgccc
     2521 gacgcccact tcgtggacca ccgcatcgag atcctgggca acgacagcga ctacaacaag
     2581 gtgaagctgt acgagcacgc cgtggcccgc tacagccccc tgcccagcca ggtgtggtaa
     2641 ccggtccacc accaccacca ccactaagga tccggggttg gggttgcgcc ttttccaagg
     2701 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
     2761 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
     2821 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
     2881 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
     2941 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
     3001 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
     3061 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     3121 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     3181 ccagcaattc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     3241 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     3301 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     3361 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     3421 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     3481 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     3541 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     3601 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     3661 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     3721 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     3781 gcccggtgcc gaaggtagag gttctctcct cacttgtggt gatgttgaag aaaaccctgg
     3841 tccaatgtct agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg
     3901 tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     3961 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     4021 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     4081 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     4141 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     4201 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     4261 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     4321 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     4381 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     4441 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     4501 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     4561 cgattttgac cttgacatgc tccccgggta actaagtaag gatcgatcca agatatcgta
     4621 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4681 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4741 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4801 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4861 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4921 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     4981 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5041 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5101 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5161 ccccgcctgt ttcgcctcgg cgtccggact agaggtacct ttaagaccaa tgacttacaa
     5221 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5281 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5341 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5401 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5461 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5521 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     5581 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5641 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5701 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5761 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5821 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctttc aaatatgtat
     5881 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5941 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6001 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6061 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6121 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6181 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6241 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6301 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6361 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6421 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6481 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6541 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6601 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6661 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6721 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6781 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6841 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6901 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6961 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7021 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7081 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7141 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7201 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7261 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7321 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7381 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7441 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7501 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7561 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7621 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7681 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7741 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7801 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7861 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7921 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     7981 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     8041 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     8101 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     8161 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     8221 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     8281 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8341 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8401 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     8461 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     8521 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     8581 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     8641 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     8701 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     8761 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     8821 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     8881 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     8941 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     9001 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     9061 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     9121 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     9181 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     9241 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     9301 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     9361 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     9421 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     9481 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     9541 tagataaatg ggcaagttt
//
