LOCUS       sequence-235827-        9142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  conditionally knockdown Rab27a in mouse cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Yang Y, Li CW, Chan LC, Wei Y, Hsu JM, Xia W, Cha JH, Hou J, Hsu JL,
            Sun L, Hung MC
  TITLE     Exosomal PD-L1 harbors active defense function to suppress T cell 
            killing of breast cancer cells and promote tumor growth.
  JOURNAL   Cell Res. 2018 Aug;28(8):862-864. doi: 10.1038/s41422-018-0060-4. 
            Epub 2018 Jun 29.
  PUBMED    29959401
REFERENCE   2  (bases 1 to 9142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-018-0060-4"; journalName: "Cell Res"; date: 
            "2018-08"; volume: "28"; issue: "8"; pages: "862-864"
COMMENT     Sequence Label: pLKO-Tet-On-shRab27a
FEATURES             Location/Qualifiers
     source          1..9142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     161..178
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    295..316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(331..336,337..354,355..361)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    369..385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     374..396
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     393..409
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     393..409
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     428..448
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        430..448
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        476..702
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             703..883
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    930..1055
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1548..1781
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1966..2010
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2299..2393
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2310..2329
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2347..2365
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2375..2393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2494..2611
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2660..3170
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3089..3107
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3179..3751
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3759..3778
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3806..3824
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3835..4455
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3908..3927)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4490..5063
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4657..4674)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4884..4903
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5064..5864
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5124..5143)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5734..5753
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(5893..5992)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             6136..6369
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6441..6562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6478..6497)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6532..6551
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6602..6737
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6758..6776)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6786..6803)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6786..6802)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6795..6817)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6944..7399
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7031..7050)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7241..7262
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7425..7529
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7530..7598,7599..8390)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7748..7767)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8561..9142,1..7)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9050..9069
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
       61 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      121 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      181 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      241 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      301 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      361 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      421 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
      481 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
      541 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
      601 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
      661 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
      721 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      781 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      841 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      901 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      961 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     1021 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     1081 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     1141 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     1201 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1261 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1321 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1381 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1441 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1501 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1561 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1621 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1681 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1741 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1801 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1861 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1921 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1981 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     2041 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     2101 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2161 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     2221 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg atcacgagac
     2281 tagcctcgag cggccgcaat atttgcatgt cgctatgtgt tctgggaaat caccataaac
     2341 gtgaaatccc tatcagtgat agagacttat aagttcccta tcagtgatag agacaccggg
     2401 cttctgttcg acctgacaaa ctcgagtttg tcaggtcgaa cagaagcttt ttgaattctc
     2461 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2521 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2581 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2641 actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg
     2701 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     2761 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     2821 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     2881 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     2941 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     3001 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     3061 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     3121 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatctgt
     3181 gagtttgggg acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg
     3241 agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg
     3301 accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct
     3361 tattttcttt tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat
     3421 ttttaaattc acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt
     3481 tcaaggcaat cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat
     3541 aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct
     3601 tattggtaga aacaactaca tcctggtcat catcctgcct ttctctttat ggttacaatg
     3661 atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa
     3721 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     3781 gtctcatcat tttggcaaag aattgtaata cgactcacta tagggcgaat tgatatgtct
     3841 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc
     3901 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat
     3961 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg
     4021 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac
     4081 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt
     4141 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa
     4201 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta
     4261 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact
     4321 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca
     4381 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa
     4441 tgtgaaagtg ggtccgcgta cagcggctcc cgggagttct agggatctgc ccctctccct
     4501 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     4561 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     4621 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     4681 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     4741 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     4801 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     4861 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     4921 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     4981 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     5041 tcctttgaaa aacacgatga taaggatcca tgattgaaca agatggattg cacgcaggtt
     5101 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5161 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5221 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     5281 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5341 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5401 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5461 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5521 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5581 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     5641 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5701 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5761 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5821 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggtacc ttttttaagg
     5881 gtgggcgcgc cgacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat
     5941 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     6001 ctgatatccg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     6061 catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6121 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     6181 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6241 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6301 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6361 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     6421 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     6481 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6541 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     6601 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6661 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6721 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     6781 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6841 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6901 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     6961 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7021 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7081 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7141 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7201 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7261 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7321 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7381 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7441 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7501 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7561 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7621 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7681 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7741 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7801 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7861 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7921 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7981 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8041 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8101 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8161 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8221 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8281 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8341 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8401 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8461 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8521 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8581 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8641 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8701 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8761 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8821 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8881 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8941 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9001 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9061 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9121 gctcgtcagg ggggcggagc ct
//
