LOCUS       sequence-235832-        9624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer plasmid for expression of TVA950.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Jin L, Matsuyama M, Sullivan HA, Zhu M, Lavin TK, Hou Y, Lea NE, 
            Pruner MT, Dam Ferdinez ML, Wickersham IR
  TITLE     ""Self-inactivating"" rabies viruses are susceptible to loss of 
            their intended attenuating modification.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Feb 14;120(7):e2023481120. doi: 
            10.1073/pnas.2023481120. Epub 2023 Feb 6.
  PUBMED    37053554
REFERENCE   2  (bases 1 to 9624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-02-14"; volume: "120(7):e2023481120. 
            doi"; pages: " 10.1073/pnas.2023481120. Epub 2023 Feb 6"
COMMENT     Sequence Label: pLV-U-TVA950
FEATURES             Location/Qualifiers
     source          1..9624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        150..529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        531..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     684..704
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             747..927
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    974..1099
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1592..1825
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2010..2054
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2306..2423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2436..3647
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3484..3503
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3730..3739
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4346..4934
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4399..4419)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4817..4828)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5341..5521
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5547..5564)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5553..5777
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5823..6251
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5910..5929)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6120..6141
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6260..6280)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6265..6594
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6445..6580
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6507..6526
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6642..6689
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6708..7082
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7212..7333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7249..7268)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7303..7322
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7382..7398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7382..7398)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7395..7417)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7406..7422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7430..7436,7437..7454,7455..7460))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7475..7496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7613..7630)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7784..8372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7864..7883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8543..9334,9335..9403))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9166..9185
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9404..9508)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9583..9602)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
       61 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      121 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
      181 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      241 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      301 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      361 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      421 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      481 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      541 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      601 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      661 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      721 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
      781 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      841 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      901 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      961 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     1021 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     1081 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     1141 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1201 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1261 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1321 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1381 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1441 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1501 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1561 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1621 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1681 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1741 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1801 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1861 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1921 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1981 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     2041 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     2101 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     2161 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2221 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2281 ccattcgatt agtgaacgga tcaactttta aaagaaaagg ggggattggg gggtacagtg
     2341 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     2401 aaattacaaa aattcaaaat tttatcgatg gatctggcct ccgcgccggg ttttggcgcc
     2461 tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga
     2521 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
     2581 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
     2641 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
     2701 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt
     2761 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg
     2821 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg
     2881 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga
     2941 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag
     3001 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
     3061 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
     3121 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
     3181 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca
     3241 cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt
     3301 ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga
     3361 cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag
     3421 gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt
     3481 agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt
     3541 tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta
     3601 gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttgggctg
     3661 caggtcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggccgc gggcccaaaa
     3721 gaattcgccg ccaccatggc gcggctgctg cccgcgctgc tgctgctgct gctgcccggt
     3781 aacgtgaccg gtaacgggtc cggtaacggt tctttgtccc gttgcccccc cggtcagttc
     3841 cgctgctcgg agccgcccgg tgcccacggg gagtgttacc cgcaggactg gctgtgcgac
     3901 ggacaccccg actgcgacga cgggcgggac gagtggggct gcgggaccag cgcgatcccc
     3961 gcggtgccca cggacaacgg cacagaggct cccactgccc ctgctcctgg acgtgctctg
     4021 ccagccagga atcacggccg catgtggatg ctgatcactg cagtgctcct gtgctgcctg
     4081 gtagctgtgg gtggtatcgc tgcatggggg aagtccaaag caaaaagcag gtctgacatc
     4141 ttcagtcttg aaagcgcatc caaggagctg ctggtgcctg acaagagcca ggcagacttg
     4201 ttctcctgag ggccctcgag aaaagtttaa acctggaaaa acatggagca atcacaagta
     4261 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     4321 tgggttttcc agtcacacct cagacaatca acctctggat tacaaaattt gtgaaagatt
     4381 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4441 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4501 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4561 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4621 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4681 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4741 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4801 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4861 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4921 ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     4981 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5041 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     5101 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     5161 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     5221 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     5281 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     5341 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     5401 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     5461 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     5521 agggccgctt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5581 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5641 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5701 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5761 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     5821 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5881 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5941 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6001 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6061 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6121 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6181 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6241 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     6301 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     6361 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6421 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6481 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     6541 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     6601 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     6661 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     6721 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     6781 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     6841 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     6901 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     6961 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     7021 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     7081 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     7141 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     7201 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7261 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7321 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     7381 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7441 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7501 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7561 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7621 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7681 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7741 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7801 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7861 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7921 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7981 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8041 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8101 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8161 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8221 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8281 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8341 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8401 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8461 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8521 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8581 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8641 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8701 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8761 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8821 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8881 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8941 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9001 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9061 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9121 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9181 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9241 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9301 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9361 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9421 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9481 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     9541 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     9601 cgcatagtta agccagtatc tgct
//
