LOCUS       sequence-235849-        9419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses E. coli codon optimized Cas12i2 effector in the pET28a 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Yan WX, Hunnewell P, Alfonse LE, Carte JM, Keston-Smith E, 
            Sothiselvam S, Garrity AJ, Chong S, Makarova KS, Koonin EV, Cheng 
            DR, Scott DA
  TITLE     Functionally diverse type V CRISPR-Cas systems.
  JOURNAL   Science. 2019 Jan 4;363(6422):88-91. doi: 10.1126/science.aav7271. 
            Epub 2018 Dec 6.
  PUBMED    30523077
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aav7271"; journalName: "Science"; date: "2019-01-4-
            4"; volume: "363"; issue: "6422"; pages: "88-91"
COMMENT     Sequence Label: pET28a-mH6-Cas12i2
FEATURES             Location/Qualifiers
     source          1..9419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..68
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(62..253)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(1062..2144)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2106..2125
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2145..2222)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     2428..2447
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2531..2550
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2531..2549
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2550..2574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(2583..2588,2589..2606,2607..2612)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             2632..2654
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2686..2703
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6884..6902)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6898..6945
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6982..7437
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7069..7088)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7279..7300
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7529..8344)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7529..8344)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8252..8271
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8336..8355
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8466..9054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8955..8974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9208..9225
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9240..9379
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9305..9324)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
       61 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
      121 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
      181 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
      241 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac
      301 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
      361 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
      421 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc
      481 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca
      541 gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt
      601 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
      661 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
      721 gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
      781 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
      841 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
      901 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
      961 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     1021 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     1081 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1141 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     1201 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     1261 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     1321 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
     1381 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     1441 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     1501 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     1561 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     1621 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     1681 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     1741 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     1801 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     1861 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     1921 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     1981 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     2041 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     2101 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     2161 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     2221 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     2281 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
     2341 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     2401 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     2461 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat
     2521 cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta
     2581 gatttacact ttatgcttcc ggctcgtatg tttctagaaa gcttaaataa ttttgtttaa
     2641 ctttaagaag gagatttaaa tatgaaaatc gaagaaggta aaggtcacca tcaccatcac
     2701 cacatgagca gcgcgatcaa aagctacaag agcgttctgc gtccgaacga gcgtaagaac
     2761 caactgctga aaagcaccat tcagtgcctg gaagacggta gcgcgttctt tttcaagatg
     2821 ctgcaaggcc tgtttggtgg catcaccccg gagattgttc gtttcagcac cgaacaggag
     2881 aaacagcaac aggatatcgc gctgtggtgc gcggttaact ggttccgtcc ggtgagccaa
     2941 gacagcctga cccacaccat tgcgagcgat aacctggtgg agaagtttga ggaatactat
     3001 ggtggcaccg cgagcgacgc gatcaaacag tacttcagcg cgagcattgg cgaaagctac
     3061 tattggaacg actgccgtca acagtactat gatctgtgcc gtgagctggg tgttgaggtg
     3121 agcgacctga cccatgatct ggagatcctg tgccgtgaaa agtgcctggc ggttgcgacc
     3181 gagagcaacc agaacaacag catcattagc gttctgtttg gcaccggcga aaaagaggac
     3241 cgtagcgtga aactgcgtat caccaagaaa attctggagg cgatcagcaa cctgaaagaa
     3301 atcccgaaga acgttgcgcc gattcaagag atcattctga acgtggcgaa agcgaccaag
     3361 gaaaccttcc gtcaggtgta tgcgggtaac ctgggtgcgc cgagcaccct ggagaaattt
     3421 atcgcgaagg acggccaaaa agagttcgat ctgaagaaac tgcagaccga cctgaagaaa
     3481 gttattcgtg gtaaaagcaa ggagcgtgat tggtgctgcc aggaagagct gcgtagctac
     3541 gtggagcaaa acaccatcca gtatgacctg tgggcgtggg gcgaaatgtt caacaaagcg
     3601 cacaccgcgc tgaaaatcaa gagcacccgt aactacaact ttgcgaagca acgtctggaa
     3661 cagttcaaag agattcagag cctgaacaac ctgctggttg tgaagaagct gaacgacttt
     3721 ttcgatagcg aatttttcag cggcgaggaa acctacacca tctgcgttca ccatctgggt
     3781 ggcaaggacc tgagcaaact gtataaggcg tgggaggatg atccggcgga cccggaaaac
     3841 gcgattgtgg ttctgtgcga cgatctgaaa aacaacttta agaaagagcc gatccgtaac
     3901 attctgcgtt acatcttcac cattcgtcaa gaatgcagcg cgcaggacat cctggcggcg
     3961 gcgaagtaca accaacagct ggatcgttat aaaagccaaa aggcgaaccc gagcgttctg
     4021 ggtaaccagg gctttacctg gaccaacgcg gtgatcctgc cggagaaggc gcagcgtaac
     4081 gaccgtccga acagcctgga tctgcgtatt tggctgtacc tgaaactgcg tcacccggac
     4141 ggtcgttgga agaaacacca tatcccgttc tacgataccc gtttcttcca agaaatttat
     4201 gcggcgggca acagcccggt tgacacctgc cagtttcgta ccccgcgttt cggttatcac
     4261 ctgccgaaac tgaccgatca gaccgcgatc cgtgttaaca agaaacatgt gaaagcggcg
     4321 aagaccgagg cgcgtattcg tctggcgatc caacagggca ccctgccggt gagcaacctg
     4381 aagatcaccg aaattagcgc gaccatcaac agcaaaggtc aagtgcgtat tccggttaag
     4441 tttgacgtgg gtcgtcaaaa aggcaccctg cagatcggtg accgtttctg cggctacgat
     4501 caaaaccaga ccgcgagcca cgcgtatagc ctgtgggaag tggttaaaga gggtcaatac
     4561 cataaagagc tgggctgctt tgttcgtttc atcagcagcg gtgacatcgt gagcattacc
     4621 gagaaccgtg gcaaccaatt tgatcagctg agctatgaag gtctggcgta cccgcaatat
     4681 gcggactggc gtaagaaagc gagcaagttc gtgagcctgt ggcagatcac caagaaaaac
     4741 aagaaaaagg aaatcgtgac cgttgaagcg aaagagaagt ttgacgcgat ctgcaagtac
     4801 cagccgcgtc tgtataaatt caacaaggag tacgcgtatc tgctgcgtga tattgttcgt
     4861 ggcaaaagcc tggtggaact gcaacagatt cgtcaagaga tctttcgttt cattgaacag
     4921 gactgcggtg ttacccgtct gggcagcctg agcctgagca ccctggaaac cgtgaaagcg
     4981 gttaagggta tcatttacag ctattttagc accgcgctga acgcgagcaa gaacaacccg
     5041 atcagcgacg aacagcgtaa agagtttgat ccggaactgt tcgcgctgct ggaaaagctg
     5101 gagctgattc gtacccgtaa aaagaaacaa aaagtggaac gtatcgcgaa cagcctgatt
     5161 cagacctgcc tggagaacaa catcaagttc attcgtggtg aaggcgacct gagcaccacc
     5221 aacaacgcga ccaagaaaaa ggcgaacagc cgtagcatgg attggttggc gcgtggtgtt
     5281 tttaacaaaa tccgtcaact ggcgccgatg cacaacatta ccctgttcgg ttgcggcagc
     5341 ctgtacacca gccaccagga cccgctggtg catcgtaacc cggataaagc gatgaagtgc
     5401 cgttgggcgg cgatcccggt taaggacatt ggcgattggg tgctgcgtaa gctgagccaa
     5461 aacctgcgtg cgaaaaacat cggcaccggc gagtactatc accaaggtgt taaagagttc
     5521 ctgagccatt atgaactgca ggacctggag gaagagctgc tgaagtggcg tagcgatcgt
     5581 aaaagcaaca ttccgtgctg ggtgctgcag aaccgtctgg cggagaagct gggcaacaaa
     5641 gaagcggtgg tttacatccc ggttcgtggt ggccgtattt attttgcgac ccacaaggtg
     5701 gcgaccggtg cggtgagcat cgttttcgac caaaaacaag tgtgggtttg caacgcggat
     5761 catgttgcgg cggcgaacat cgcgctgacc gtgaagggta ttggcgaaca aagcagcgac
     5821 gaagagaacc cggatggtag ccgtatcaaa ctgcagctga ccagctaata aaagcttggt
     5881 ccattaggta gggacaatga ttcagaaagt agaaattgcg gcccaacgca ccggtttgcc
     5941 attggaacgt gactcgaagg caaaatgttc gtgatgtaat cgctttacaa acataacctt
     6001 ctgtggtaaa atattactca gttgcaaaat ccgtctttca ttgacggtgg cccggtacta
     6061 cccccgactg agaaattcaa caaccctgct acaatgcaga ctgatctttg acaatttagt
     6121 tgcaatgcct aagaaatccg tctttcattg acgggtcatg gtcctccttc tttcattgac
     6181 ggcagtggcg gaatagcggc cctaagaaat ccgtctttca ttgacggcat tcggcgctcg
     6241 aataggaaat ccgtctttca ttgacggatc attcatggtc ctccttgtgt tgcaacacct
     6301 aagaaatccg tctttcattg acgggagcca gccaaaaggc gtcgttgttg gctctgccgc
     6361 cgttgcaaca cctaagaaat ccgtctttca ttgacggggg gaaactggca acggcggact
     6421 tccagcaaca cctaagaaat ccgtctttca ttgacgggct gcccctcttg gccgttgcaa
     6481 cacctaagaa atccgtcttt cattgacggg tggcctcgac gggaacaaca ggatcgtggg
     6541 aggcccagca acacctaaga aatccgtctt tcattgacgg ttcacgccag cccagcaaca
     6601 cctaagaaat ccgtctttca ttgacgggct gcccctcttg gccgttgcaa cacctaagaa
     6661 atccgtcttt cattgacggg tcgagggatt ggaactggcg agaaatccgt ctttcattga
     6721 cgggtcgtag gcgacgaaaa gcgttgcaaa acccaagaaa tccgtctttc attggcatac
     6781 agcctcgaga gagaccatgg ccaccggtct ctatcgaaga tatgaggagc tcaaactcga
     6841 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     6901 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     6961 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7021 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7081 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7141 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     7201 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7261 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7321 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7381 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     7441 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7501 aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact
     7561 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     7621 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     7681 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     7741 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca
     7801 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     7861 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     7921 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     7981 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     8041 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     8101 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     8161 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     8221 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     8281 cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc
     8341 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa
     8401 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8461 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8521 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8581 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     8641 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8701 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8761 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8821 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8881 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8941 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9001 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9061 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9121 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9181 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9241 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc
     9301 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     9361 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacggg
//
