LOCUS       sequence-235855-        9218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9218)
  AUTHORS   Xu S, Luk K, Yao Q, Shen AH, Zeng J, Wu Y, Luo HY, Brendel C, 
            Pinello L, Chui DHK, Wolfe SA, Bauer DE
  TITLE     Editing aberrant splice sites efficiently restores beta-globin 
            expression in beta-thalassemia.
  JOURNAL   Blood. 2019 Jan 31. pii: blood-2019-01-895094. doi: 
            10.1182/blood-2019-01-895094.
  PUBMED    30704988
REFERENCE   2  (bases 1 to 9218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2019
            Jan 31. pii: blood-2019-01-895094. doi: 
            10.1182/blood-2019-01-895094."
COMMENT     Sequence Label: pET-21a_2xNLS_LbCpf1_protein_expression
FEATURES             Location/Qualifiers
     source          1..9218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     29..48
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     132..151
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        132..150
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    151..175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             190..212
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             220..3903
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             3904..3951
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3958..4038
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             4039..4059
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4081..4098
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4151..4169)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4165..4212
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4249..4704
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4336..4355)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4546..4567
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4730..4834
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4835..4903,4904..5695)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5053..5072)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5866..6454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6355..6374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6608..6625
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6640..6779
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6705..6724)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6865..6887
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6881..7072)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7881..8963)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8925..8944
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8964..9041)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
       61 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga
      121 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
      181 agaaataatt ttgtttaact ttaagaagga gatatacata tgagcaagct ggagaagttt
      241 acaaactgct actccctgtc taagaccctg aggttcaagg ccatccctgt gggcaagacc
      301 caggagaaca tcgacaataa gcggctgctg gtggaggacg agaagagagc cgaggattat
      361 aagggcgtga agaagctgct ggatcgctac tatctgtctt ttatcaacga cgtgctgcac
      421 agcatcaagc tgaagaatct gaacaattac atcagcctgt tccggaagaa aaccagaacc
      481 gagaaggaga ataaggagct ggagaacctg gagatcaatc tgcggaagga gatcgccaag
      541 gccttcaagg gcaacgaggg ctacaagtcc ctgtttaaga aggatatcat cgagacaatc
      601 ctgccagagt tcctggacga taaggacgag atcgccctgg tgaacagctt caatggcttt
      661 accacagcct tcaccggctt ctttgataac agagagaata tgttttccga ggaggccaag
      721 agcacatcca tcgccttcag gtgtatcaac gagaatctga cccgctacat ctctaatatg
      781 gacatcttcg agaaggtgga cgccatcttt gataagcacg aggtgcagga gatcaaggag
      841 aagatcctga acagcgacta tgatgtggag gatttctttg agggcgagtt ctttaacttt
      901 gtgctgacac aggagggcat cgacgtgtat aacgccatca tcggcggctt cgtgaccgag
      961 agcggcgaga agatcaaggg cctgaacgag tacatcaacc tgtataatca gaaaaccaag
     1021 cagaagctgc ctaagtttaa gccactgtat aagcaggtgc tgagcgatcg ggagtctctg
     1081 agcttctacg gcgagggcta tacatccgat gaggaggtgc tggaggtgtt tagaaacacc
     1141 ctgaacaaga acagcgagat cttcagctcc atcaagaagc tggagaagct gttcaagaat
     1201 tttgacgagt actctagcgc cggcatcttt gtgaagaacg gccccgccat cagcacaatc
     1261 tccaaggata tcttcggcga gtggaacgtg atccgggaca agtggaatgc cgagtatgac
     1321 gatatccacc tgaagaagaa ggccgtggtg accgagaagt acgaggacga tcggagaaag
     1381 tccttcaaga agatcggctc cttttctctg gagcagctgc aggagtacgc cgacgccgat
     1441 ctgtctgtgg tggagaagct gaaggagatc atcatccaga aggtggatga gatctacaag
     1501 gtgtatggct cctctgagaa gctgttcgac gccgattttg tgctggagaa gagcctgaag
     1561 aagaacgacg ccgtggtggc catcatgaag gacctgctgg attctgtgaa gagcttcgag
     1621 aattacatca aggccttctt tggcgagggc aaggagacaa acagggacga gtccttctat
     1681 ggcgattttg tgctggccta cgacatcctg ctgaaggtgg accacatcta cgatgccatc
     1741 cgcaattatg tgacccagaa gccctactct aaggataagt tcaagctgta ttttcagaac
     1801 cctcagttca tgggcggctg ggacaaggat aaggagacag actatcgggc caccatcctg
     1861 agatacggct ccaagtacta tctggccatc atggataaga agtacgccaa gtgcctgcag
     1921 aagatcgaca aggacgatgt gaacggcaat tacgagaaga tcaactataa gctgctgccc
     1981 ggccctaata agatgctgcc aaaggtgttc ttttctaaga agtggatggc ctactataac
     2041 cccagcgagg acatccagaa gatctacaag aatggcacat tcaagaaggg cgatatgttt
     2101 aacctgaatg actgtcacaa gctgatcgac ttctttaagg atagcatctc ccggtatcca
     2161 aagtggtcca atgcctacga tttcaacttt tctgagacag agaagtataa ggacatcgcc
     2221 ggcttttaca gagaggtgga ggagcagggc tataaggtga gcttcgagtc tgccagcaag
     2281 aaggaggtgg ataagctggt ggaggagggc aagctgtata tgttccagat ctataacaag
     2341 gacttttccg ataagtctca cggcacaccc aatctgcaca ccatgtactt caagctgctg
     2401 tttgacgaga acaatcacgg acagatcagg ctgagcggag gagcagagct gttcatgagg
     2461 cgcgcctccc tgaagaagga ggagctggtg gtgcacccag ccaactcccc tatcgccaac
     2521 aagaatccag ataatcccaa gaaaaccaca accctgtcct acgacgtgta taaggataag
     2581 aggttttctg aggaccagta cgagctgcac atcccaatcg ccatcaataa gtgccccaag
     2641 aacatcttca agatcaatac agaggtgcgc gtgctgctga agcacgacga taacccctat
     2701 gtgatcggca tcgatagggg cgagcgcaat ctgctgtata tcgtggtggt ggacggcaag
     2761 ggcaacatcg tggagcagta ttccctgaac gagatcatca acaacttcaa cggcatcagg
     2821 atcaagacag attaccactc tctgctggac aagaaggaga aggagaggtt cgaggcccgc
     2881 cagaactgga cctccatcga gaatatcaag gagctgaagg ccggctatat ctctcaggtg
     2941 gtgcacaaga tctgcgagct ggtggagaag tacgatgccg tgatcgccct ggaggacctg
     3001 aactctggct ttaagaatag ccgcgtgaag gtggagaagc aggtgtatca gaagttcgag
     3061 aagatgctga tcgataagct gaactacatg gtggacaaga agtctaatcc ttgtgcaaca
     3121 ggcggcgccc tgaagggcta tcagatcacc aataagttcg agagctttaa gtccatgtct
     3181 acccagaacg gcttcatctt ttacatccct gcctggctga catccaagat cgatccatct
     3241 accggctttg tgaacctgct gaaaaccaag tataccagca tcgccgattc caagaagttc
     3301 atcagctcct ttgacaggat catgtacgtg cccgaggagg atctgttcga gtttgccctg
     3361 gactataaga acttctctcg cacagacgcc gattacatca agaagtggaa gctgtactcc
     3421 tacggcaacc ggatcagaat cttccggaat cctaagaaga acaacgtgtt cgactgggag
     3481 gaggtgtgcc tgaccagcgc ctataaggag ctgttcaaca agtacggcat caattatcag
     3541 cagggcgata tcagagccct gctgtgcgag cagtccgaca aggccttcta ctctagcttt
     3601 atggccctga tgagcctgat gctgcagatg cggaacagca tcacaggccg caccgacgtg
     3661 gattttctga tcagccctgt gaagaactcc gacggcatct tctacgatag ccggaactat
     3721 gaggcccagg agaatgccat cctgccaaag aacgccgacg ccaatggcgc ctataacatc
     3781 gccagaaagg tgctgtgggc catcggccag ttcaagaagg ccgaggacga gaagctggat
     3841 aaggtgaaga tcgccatctc taacaaggag tggctggagt acgcccagac cagcgtgaag
     3901 cacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac
     3961 ccatacgatg ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat
     4021 gatgtccccg actatgcccc aaagaagaag cggaaggtca agcttgcggc cgcactcgag
     4081 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
     4141 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     4201 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
     4261 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4321 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4381 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4441 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     4501 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4561 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4621 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4681 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4741 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4801 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4861 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4921 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4981 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5041 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5101 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5161 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5221 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5281 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5341 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     5401 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5461 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5521 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5581 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5641 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5701 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5761 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5821 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5881 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5941 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6001 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6061 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6121 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6181 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6241 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6301 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6361 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6421 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6481 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6541 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6601 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     6661 tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat
     6721 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     6781 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     6841 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     6901 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
     6961 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     7021 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     7081 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
     7141 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     7201 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     7261 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
     7321 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
     7381 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
     7441 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
     7501 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa
     7561 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
     7621 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
     7681 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
     7741 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
     7801 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac
     7861 ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7921 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg
     7981 gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga
     8041 gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg
     8101 gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag
     8161 atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc
     8221 tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt
     8281 tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga
     8341 ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat
     8401 gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt
     8461 cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca
     8521 agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc
     8581 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct
     8641 ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga
     8701 tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag
     8761 gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga
     8821 atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg
     8881 ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca
     8941 tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat
     9001 catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc
     9061 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     9121 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     9181 tgccaccata cccacgccga aacaagcgct catgagcc
//
