LOCUS       sequence-235857-        8903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of a germ-line targeted, codon optimized Cas9 for 
            genome-editing in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Moreno-Mateos MA, Vejnar CE, Beaudoin JD, Fernandez JP, Mis EK, 
            Khokha MK, Giraldez AJ
  TITLE     CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 
            targeting in vivo.
  JOURNAL   Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3543.
  PUBMED    26322839
REFERENCE   2  (bases 1 to 8903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Aug 31. doi: 10.1038/nmeth.3543."
COMMENT     Sequence Label: pCS2-nCas9n-nanos 3'UTR
FEATURES             Location/Qualifiers
     source          1..8903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             117..137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             144..4244
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes MGAS15252 Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFGSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQIYNQLFEENPINASRVDAKAILSARLSKSRRL
                     ENLIAQLPGEKRNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGAYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDRGMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGHSLHEQIANLAGSPAIKKGILQTVKIVDELVKVMGH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             4157..4165
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4260..4280
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4955..5089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4979..4998)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5033..5052
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5202..5222)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5202..5220)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5241..5257)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5241..5257)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5254..5276)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5265..5281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5289..5295,5296..5313,5314..5319))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5334..5355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5472..5489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5643..6231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5723..5742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6402..7193,7194..7262))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7025..7044
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7263..7367)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7393..7848)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7530..7551)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7742..7761
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7919..8903
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccgatcca atgtcgacgc caccatggct tctccaccta
      121 agaagaagag aaaggtggga agcatggata agaagtatag catcggcctg gatattggaa
      181 ctaactccgt gggttgggca gtgattacag acgactacaa ggtccctagc aagaaattta
      241 aggtgctggg taacaccgac aggcacagca tcaagaaaaa tctgattgga gccctgctgt
      301 tcggttctgg agagactgcc gaagcaacac gcctgaaaag aacagcaaga aggcgctata
      361 ccagaaggaa gaatagaatc tgttacctgc aggagatttt ctctaacgaa atggctaagg
      421 tggacgattc attctttcat aggctggagg aaagtttcct ggtcgaggaa gataagaaac
      481 acgagcgcca tcctatcttt ggaaacattg tggacgaggt cgcctatcac gaaaaatacc
      541 caaccatcta tcatctgcgc aagaaactgg ctgactctac tgataaagcc gacctgagac
      601 tgatctatct ggctctggcc cacatgatta agttcagggg tcattttctg atcgagggcg
      661 atctgaaccc cgacaattcc gatgtggaca agctgttcat ccagctggtc cagatttaca
      721 atcagctgtt tgaggaaaac cctattaatg cttccagagt ggacgcaaaa gctatcctgt
      781 cagccaggct gtccaagtca cgcagactgg agaacctgat tgcacagctg cccggagaaa
      841 agaggaacgg tctgtttgga aatctgatcg ctctgagtct gggcctgact cctaacttca
      901 aaagcaattt tgatctggct gaggacgcca aactgcagct gtcaaaggac acatatgacg
      961 atgacctgga taacctgctg gcacagatcg gagatcagta cgctgacctg ttcctggctg
     1021 ccaaaaatct gtccgacgca atcctgctgt cagatattct gagagtgaac agcgagatta
     1081 caaaagctcc tctgagtgcc agcatgatca agagatatga cgagcaccat caggatctga
     1141 ccctgctgaa ggctctggtc aggcagcagc tgccagagaa gtacaaggaa attttctttg
     1201 atcagtccaa gaacggctac gccggttata tcgacggagg cgcatcacag gaggaattct
     1261 acaagtttat caaacctatt ctggagaaga tggacggaac tgaggaactg ctggtgaaac
     1321 tgaatagaga ggacctgctg aggaagcagc gcacatttga taacggttcc atcccacacc
     1381 agattcatct gggagagctg cacgctatcc tgaggcgcca ggaagacttc tacccctttc
     1441 tgaaagataa ccgcgagaag atcgaaaaaa ttctgacctt cagaatccct tactatgtgg
     1501 gtccactggc tcgcggaaac agcagatttg cctggatgac tcgcaaatcc gaggaaacca
     1561 ttactccttg gaacttcgag gaagtggtcg ataagggcgc ctctgcacag tccttcatcg
     1621 agagaatgac taattttgac aaaaacctgc ccaatgagaa agtgctgcct aagcactccc
     1681 tgctgtacga gtatttcact gtctataacg aactgacaaa ggtgaaatac gtcaccgagg
     1741 gcatgagaaa gccagccttc ctgtcaggag agcagaagaa agcaatcgtg gatctgctgt
     1801 ttaaaaccaa caggaaagtg actgtcaagc agctgaagga ggactacttc aagaaaattg
     1861 aatgcttcga ttccgtggag atcagcggag tcgaagacag atttaacgca agcctgggcg
     1921 cttaccacga tctgctgaag atcattaagg ataaagactt cctggacaac gaggaaaatg
     1981 aggatatcct ggaagacatt gtgctgacac tgaccctgtt tgaggacaga ggaatgatcg
     2041 aggaaagact gaaaacctat gctcatctgt tcgatgacaa ggtgatgaaa cagctgaaga
     2101 gaaggcgcta cactggctgg ggtagactga gcaggaagct gatcaacggc attagggata
     2161 aacagtcagg aaagacaatc ctggactttc tgaaaagtga tggcttcgcc aaccgcaatt
     2221 ttatgcagct gattcacgat gacagtctga ccttcaaaga ggacatccag aaggctcagg
     2281 tgtctggaca gggccactcc ctgcatgagc agattgcaaa cctggctgga agcccagcca
     2341 tcaagaaagg cattctgcag acagtgaaaa tcgtcgatga gctggtgaaa gtcatgggcc
     2401 ataagcccga aaacatcgtg attgagatgg ctcgcgaaaa tcagacaacc cagaagggtc
     2461 agaagaacag tagagagagg atgaaaagaa tcgaggaagg cattaaggag ctgggtagcc
     2521 agatcctgaa agagcaccca gtggaaaaca cacagctgca gaatgagaag ctgtatctgt
     2581 actatctgca gaatggaaga gatatgtacg tggaccagga gctggatatt aacaggctgt
     2641 ctgattacga cgtggatcat atcgtccccc agagtttcat caaagatgac agcattgaca
     2701 acaaggtgct gaccaggtcc gacaaaaaca gaggaaaatc agataatgtc cctagtgagg
     2761 aagtggtcaa gaaaatgaag aactactgga gacagctgct gaatgccaaa ctgatcactc
     2821 agaggaagtt tgataacctg acaaaagcag agcgcggtgg actgtcagaa ctggacaaag
     2881 ctggattcat caagaggcag ctggtggaaa cacgccagat cactaaacac gtcgcacaga
     2941 ttctggatag tcgcatgaac acaaagtacg atgagaatga caaactgatc agagaagtga
     3001 aggtcattac cctgaagagt aaactggtca gcgactttag gaaagatttc cagttttata
     3061 aggtccgcga gattaacaat tatcaccatg cccatgacgc atacctgaac gccgtggtcg
     3121 gtaccgcact gatcaagaaa tacccaaaac tggagagcga attcgtgtac ggagactata
     3181 aggtgtacga tgtcagaaaa atgatcgcca agtccgagca ggaaattgga aaagctactg
     3241 ccaagtattt cttttactca aacatcatga atttctttaa gacagagatc accctggcca
     3301 atggagaaat ccgcaaaagg cccctgattg agacaaacgg agagacaggc gaaatcgtgt
     3361 gggacaaagg cagagatttt gcaaccgtga ggaaggtcct gagcatgcct caagtgaata
     3421 tcgtcaagaa aactgaggtg cagacaggcg gtttctcaaa agaaagtatt ctgccaaaac
     3481 gcaactctga taagctgatc gctagaaaga aagactggga ccctaagaag tatggaggct
     3541 ttgactctcc cactgtggca tactccgtcc tggtggtcgc taaggtggag aagggcaaaa
     3601 gcaagaaact gaaatctgtc aaggagctgc tgggtatcac aattatggag agaagctctt
     3661 tcgagaagaa cccaatcgat tttctggagg ccaaaggtta taaggaagtg aagaaagacc
     3721 tgatcattaa actgcccaag tacagtctgt ttgagctgga aaacggcagg aaacgcatgc
     3781 tggcaagcgc tggagagctg cagaaaggca atgaactggc cctgccttct aagtacgtga
     3841 acttcctgta tctggcaagc cactacgaga agctgaaagg atctccagag gataacgaac
     3901 agaaacagct gtttgtggag cagcacaagc attatctgga cgagatcatt gaacagatta
     3961 gcgagttctc taaaagagtg atcctggccg acgcaaatct ggataaggtc ctgtctgctt
     4021 acaacaaaca cagagataag cccatcaggg agcaggccga aaatatcatt catctgttca
     4081 ctctgacaaa cctgggcgca cctgcagctt tcaagtactt cgacactaca atcgatagaa
     4141 agaggtacac ctccactaag gaggtgctgg acgctacact gatccatcag agtattaccg
     4201 gcctgtacga aacaaggatt gacctgtctc agctgggtgg cgactcaccg gtgagatctc
     4261 ctaagaagaa gagaaaggtg tgaccgcgga atctagaact atagtgagtc gtattactca
     4321 ccggtagcgg acattgatgc tccgggagat ttgaagaaac actttttacc gcaggtttta
     4381 atgtttaagt tttaactctt taattgtttg tttggttgat acgcggcgga ttgcgagttt
     4441 gcatgcatgt gtgcgttcac tgtttgattt tgcacttttt ttgtgtgtgt gtatatgtgt
     4501 gtgtttgctg tgttttattt tgtgtgcact ggtgttgtgt tttcacttgg taacaaactc
     4561 gtacacaagc cagcaggctc gctacaggcg caaccgcact caaaaacaaa ccctttcatg
     4621 cttatttggt aaatacaatg tgtgtttagt cctcctttta aatgtcagat tttatggtgt
     4681 tgtatttaaa caaaaaattc aatgttaata tttagatttt agtgatttta ttattgaaaa
     4741 cggcttgttt tgtataagta acctttaaaa aaagttttct ccattgcatt taaattcagc
     4801 ttgaaaaaca taatcgccat attttcatgt cgcttgctaa aattcatgta ctactttcat
     4861 cattttatgt cagtgtgtga tttttgactt gtgatggagt gaaaaatgtg aggaaaatat
     4921 aaacatctag aactatagtg agtcgtatta cgtagatcca gacatgataa gatacattga
     4981 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     5041 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     5101 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc
     5161 cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg agggttaatt
     5221 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5281 attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc ctaatgagtg
     5341 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5401 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5461 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5521 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5581 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5641 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5701 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5761 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5821 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5881 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5941 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6001 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6061 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6121 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6181 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6241 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6301 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6361 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6421 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6481 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6541 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6601 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6661 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6721 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6781 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6841 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6901 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6961 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7021 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7081 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7141 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7201 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7261 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7321 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7381 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     7441 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     7501 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     7561 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     7621 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     7681 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     7741 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     7801 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     7861 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt
     7921 cgaccgccaa ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg tggatctgga
     7981 cctgtgccaa ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga
     8041 ccccagccaa ttcaatatgg cggacttggc accatgccaa ttcaatatgg cggacctggc
     8101 actgtgccaa ctggggaggg gtctacttgg cacggtgcca agtttgagga ggggtcttgg
     8161 ccctgtgcca agtccgccat attgaattgg catggtgcca ataatggcgg ccatattggc
     8221 tatatgccag gatcaatata taggcaatat ccaatatggc cctatgccaa tatggctatt
     8281 ggccaggttc aatactatgt attggcccta tgccatatag tattccatat atgggttttc
     8341 ctattgacgt agatagcccc tcccaatggg cggtcccata taccatatat ggggcttcct
     8401 aataccgccc atagccactc ccccattgac gtcaatggtc tctatatatg gtctttccta
     8461 ttgacgtcat atgggcggtc ctattgacgt atatggcgcc tcccccattg acgtcaatta
     8521 cggtaaatgg cccgcctggc tcaatgccca ttgacgtcaa taggaccacc caccattgac
     8581 gtcaatggga tggctcattg cccattcata tccgttctca cgccccctat tgacgtcaat
     8641 gacggtaaat ggcccacttg gcagtacatc aatatctatt aatagtaact tggcaagtac
     8701 attactattg gaagtacgcc agggtacatt ggcagtactc ccattgacgt caatggcggt
     8761 aaatggcccg cgatggctgc caagtacatc cccattgacg tcaatgggga ggggcaatga
     8821 cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata
     8881 taagcaatgc tcgtttaggg aac
//
