LOCUS       sequence-235864-       16818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16818)
  AUTHORS   Dong XY, Sun X, Guo P, Li Q, Sasahara M, Ishii Y, Dong JT
  TITLE     ATBF1 inhibits estrogen receptor (ER) function by selectively 
            competing with AIB1 for binding to the ER in ER-positive breast 
            cancer cells.
  JOURNAL   J Biol Chem. 2010 Oct 22;285(43):32801-9. Epub 2010 Aug 18.
  PUBMED    20720010
REFERENCE   2  (bases 1 to 16818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2010-10-22"; volume: "285(43)"; pages: "32801-9. Epub
            2010 Aug 18"
COMMENT     Sequence Label: pcDNA3-FLAG-ATBF1
FEATURES             Location/Qualifiers
     source          1..16818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             763..786
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(12074..12090)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(12091..12115)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(12137..12155)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12138..12155)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(12175..12192)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12181..12405
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12451..12879
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12538..12557)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12748..12769
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12888..12908)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12893..13222
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13073..13208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13135..13154
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             13289..14083
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13343..13362)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13953..13972
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    14257..14378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14294..14313)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14348..14367
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(14427..14443)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14440..14462)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14451..14467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14475..14481,14482..14499,14500..14505))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14520..14541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14658..14675)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14829..15417)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14909..14928)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15588..16379,16380..16448))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16211..16230
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16449..16553)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(16628..16647)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattct gcagatatcc
      721 atcacactgg cggccgctcg agcatgcatc tagaccacca tggattacaa ggatgacgac
      781 gataaggaag gctgtgactc gcccgtcgtc tcggggaagg acaatgggtg cggtatccct
      841 cagcaccagc aatggactga actcaacagc acccacctcc ctgacaaacc cagtagcatg
      901 gaacagtcca caggcgagag ccacgggccc ttggacagcc tgagggcccc cttcaatgag
      961 cgcctcgcgg agagcaccgc gtcggccggg ccccccgccg agcccgccag caaggaggtc
     1021 acctgcaacg aatgttcggc ctcctttgcc agcctccaga cctacatgga gcaccactgc
     1081 cccagcgcgc gccccccgcc acccctgaga gaggagagcg ctagcgacac cggtgaggag
     1141 ggggacgagg agagtgacgt ggagaacctg gccggggaga tcgtctacca gccggacggc
     1201 tccgcataca ttgtggagag cctgagccag ctgacccagg gcgggggcgc ctgtgggagt
     1261 ggcagtggca gtgggcctct cccctcgctt ttcctgaact ctctccctgg cgcggggggc
     1321 aagcaagggg acccttcgtg tgctgcaccc gtgtacccgc agatcatcaa cactttccac
     1381 atagcctcat ccttcgggaa atggtttgag ggcccagacc aggctttccc gaatacctca
     1441 gccctggcgg ggctcagccc cgtcctgcac agcttccgcg tgtttgacgt gcgacacaaa
     1501 agcaacaagg attacctgaa cagcgacggt tctgccaaaa gctcctgcgt atccaaagat
     1561 gttcccaaca atgtggacct gtccaaattc gatggctttg tgctctatgg caagaggaag
     1621 cccatcctga tgtgtttctt gtgcaaactc tcctttgggt acgtccgttc gtttgtgacc
     1681 cacgcggtgc atgaccatcg aatgaccctg agcgaagacg agcggaaaat tcttagcaat
     1741 aagaacatct ccgctatcat ccaagggatc ggcaaagaca aggaacccct tgtaagtttt
     1801 ctggaaccaa aaaacaaaaa ctttcaacac cctttagttt ccacagctaa cctcataggc
     1861 cccggacaca gtttttatgg taaatttagt ggcattcgaa tggaagggga ggaagctctc
     1921 ccagcgggct ccgccgctgg ccccgagcag ccccaggctg gtctcttgac ccccagcacc
     1981 ctgttgaacc ttggcgggct caccagctcg gtactgaaga cccccattac ctcagtcccc
     2041 ctggggcctc tggcttccag tcctaccaaa tcctcagagg gcaaggactc tggggcggca
     2101 gaaggagaga agcaggaagt gggcgacggg gattgcttct ctgagaaggt agagccagcc
     2161 gaagaggagg cggaggagga agaggaggag gaagaggcgg aggaggagga ggaagaagag
     2221 gaggaggaag aagaggagga ggaagacgag ggttgcaaag gactctttcc aagcgagttg
     2281 gatgaggaac tggaggacag gccccatgag gagcctgggg ccgcagcagg tagtagcagc
     2341 aaaaaggacc ttgctctctc aaaccaaagc atttctaact cccccttaat gcctaacgtg
     2401 ctccagaccc tgtcgagggg cacagcttct actagttcta attctgcttc ttcctttgtt
     2461 gtctttgatg gtgcgaacag gaggaatcgt ttaagcttta acagtgaggg cgtcagggcc
     2521 aatgtggcag agggcggcag gaggctggac ttcgctgacg aaagtgccaa taaagacaat
     2581 gccacagcac cagaaccaaa tgaaagtaca gagggtgacg atgggggctt cgttccccat
     2641 caccagcacg ctggctccct ctgcgagctt ggggttgggg agtgcccctc ggggagtggc
     2701 gtggagtgcc ccaaatgcga cacggtcctg ggctcctccc gctcgctggg cggccacatg
     2761 accatgatgc attctcgtaa ctcgtgtaag acactcaagt gccccaagtg caactggcac
     2821 tataagtacc agcagaccct ggaggcacac atgaaggaga agcacccgga gccggggggc
     2881 tcctgtgtct actgcaaaag cgggcagccc cacccccggc tggcacgagg cgagagctac
     2941 acgtgtggtt acaagccttt ccgctgcgag gtgtgtaact actccacaac taccaaaggc
     3001 aacctcagta ttcatatgca gtctgacaag catctcaaca acatgcagaa cctgcagaat
     3061 ggaggggggg agcaggtctt cagccacact gccggggcgg cggcggcggc ggcggctgcg
     3121 gcggcggcgg cagccaatat cagtagctcc tgcggggccc cctcgcccac caaaccaaaa
     3181 accaaaccca cctggcggtg cgaggtgtgt gattatgaga ccaacgtggc caggaacctc
     3241 cgcattcaca tgaccagtga gaagcacatg cataacatga tgttactgca acagaacatg
     3301 acccagatcc aacacaaccg ccacctgggc ctcggcagcc tgccctcacc cgccgaggcc
     3361 gagctctacc aatactacct ggcccagaac atgaacctgc ccaacctgaa gatggacagt
     3421 gctgcctcgg acgcccagtt catgatgagc ggattccagc tggatcccgc cgggcccatg
     3481 gccgccatga cgcctgctct agtgggcggt gagatccccc tagacatgcg gctcgggggc
     3541 gggcagctgg tgtcagagga gctgatgaac ctgggcgaga gcttcatcca gaccaacgac
     3601 ccgtcgctga agctcttcca gtgcgccgtc tgcaacaagt tcacgacgga caacctggac
     3661 atgctgggcc tgcacatgaa cgtggagcgc agcctgtcgg aggacgagtg gaaggcggtg
     3721 atgggggact cataccagtg caagctctgc cgctacaaca cccagctcaa ggccaacttc
     3781 cagctgcact gcaagacaga caagcacgtg cagaagtacc agctggtggc ccacatcaag
     3841 gagggcggca aggccaacga gtggaggctc aagtgtgtgg ccatcggcaa ccccgtgcac
     3901 ctcaagtgca acgcctgtga ctactacacc aacagcctgg agaagctgcg gctgcacacg
     3961 gtcaactcca ggcacgaggc cagcctgaag ttgtacaagc acctgcagca gcatgagagt
     4021 ggtgtagaag gtgagagctg ctactaccac tgcgttctgt gcaactactc caccaaggcc
     4081 aagctcaacc tcatccagca tgtgcgctcc atgaagcacc agcgaagcga gagcctgcga
     4141 aagctgcagc ggctgcagaa gggccttcca gaggaggacg aggacctggg gcagatcttc
     4201 accatccgca ggtgcccctc cacggaccca gaagaagcca ttgaagatgt tgaaggaccc
     4261 agtgaaacag ctgctgatcc agaggagctt gctaaggacc aagagggcgg agcatcgtcc
     4321 agccaagcag agaaggagct gacagattct cctgcaacct ccaaacgcat ctccttccca
     4381 ggtagctcag agtctcccct ctcttcgaag cgaccaaaaa cagctgagga gatcaaaccg
     4441 gagcagatgt accagtgtcc ctactgcaag tacagtaatg ccgatgtcaa ccggctccgg
     4501 gtgcatgcca tgacgcagca ctcggtgcaa cccatgcttc gctgccccct gtgccaggac
     4561 atgctcaaca acaagatcca cctccagctg cacctcaccc acctccacag cgtggcacct
     4621 gactgcgtgg agaagctcat tatgacggtg accacccctg agatggtgat gccaagcagc
     4681 atgttcctcc cagcagctgt tccagatcga gatgggaatt ccaatttgga agaggcagga
     4741 aagcagcctg aaacctcaga ggatctggga aagaacatct tgccatccgc aagcacagag
     4801 caaagcggag atttgaaacc atcccctgct gacccaggct ctgtgagaga agactcaggc
     4861 ttcatctgct ggaagaaggg gtgcaaccag gttttcaaaa cttctgctgc ccttcagacg
     4921 cattttaatg aagtgcatgc caagaggcct cagctgccgg tgtcagatcg ccatgtgtac
     4981 aagtaccgct gtaatcagtg tagcctggcc ttcaagacca ttgaaaagtt gcagctccat
     5041 tctcagtacc atgtgatcag agctgccacc atgtgctgtc tttgtcagcg cagtttccga
     5101 actttccagg ctctgaagaa gcaccttgag acaagccacc tggagctgag tgaggctgac
     5161 atccaacagc tttatggtgg cctgctggcc aatggggacc tcctggcaat gggagacccc
     5221 actctggctg aggaccatac cataattgtt gaggaagaca aggaggaaga gagtgacttg
     5281 gaagataaac agagcccaac gggcagtgac tctgggtcag tacaagaaga ctcgggctca
     5341 gagccaaaga gagctctgcc tttcagaaaa ggtcccaatt ttactatgga aaagttccta
     5401 gacccttctc gcccttacaa gtgtaccgtc tgcaaggaat ctttcactca aaagaatatc
     5461 ctgctagtac actacaattc tgtctcccac ctgcataagt taaagagagc ccttcaagaa
     5521 tcagcaaccg gtcagccaga acccaccagc agcccagaca acaaaccttt taagtgtaac
     5581 acttgtaatg tggcctacag ccagagttcc actctggaga tccatatgag gtctgtgtta
     5641 catcaaacca aggcccgggc agccaagctg gaggctgcaa gtggcagcag caatgggact
     5701 gggaacagca gtagtatttc cttgagctcc tccacgccaa gtcctgtgag caccagtggc
     5761 agtaacacct ttaccacctc caatccaagc agtgctggca ttgctccaag ctctaactta
     5821 ctaagccaag tgcccactga gagtgtaggg atgccacccc tggggaatcc tattggtgcc
     5881 aacattgctt ccccttcaga gcccaaagag gccaatcgga agaaactggc agatatgatt
     5941 gcatccaggc agcagcaaca acagcagcag caacagcaac aacaacaaca acaacaacaa
     6001 caacaagcac aaacgctggc ccaggcccag gctcaagttc aagctcacct gcagcaggag
     6061 ctgcagcaac aggctgccct gatccagtct cagctgttta accccaccct ccttcctcac
     6121 ttccccatga caactgagac cctgctgcaa ctacagcagc agcagcacct cctcttccct
     6181 ttctacatcc ccagtgctga gttccagctt aaccccgagg tgagcttgcc agtgaccagt
     6241 ggggcactga cactgactgg gacaggccca ggcctgctgg aagatctgaa ggctcaggtt
     6301 caggtcccac agcagagcca tcagcagatc ttgccgcagc agcagcagaa ccaactctct
     6361 atagcccaga gtcactctgc cctccttcag ccaagccagc accccgaaaa gaagaacaaa
     6421 ttggtcatca aagaaaagga aaaagaaagc cagagagaga gggacagcgc cgagggggga
     6481 gagggcaaca ccggtccgaa ggaaacactg ccagatgcct tgaaggccaa agagaagaaa
     6541 gagttggcac cagggggtgg ttctgagcct tccatgctcc ctccacgcat tgcttcagat
     6601 gccagaggga acgccaccaa ggccctgctg gagaactttg gctttgagtt ggtcatccag
     6661 tataatgaga acaagcagaa ggtgcagaaa aagaatggga agactgacca gggagagaac
     6721 ctggaaaagc tcgagtgtga ctcctgcggc aagttgtttt ccaacatctt gattttaaag
     6781 agtcatcaag agcacgttca tcagaattac tttcctttca aacagctcga gaggtttgcc
     6841 aaacagtaca gagaccacta cgataaactg tacccactga ggccccagac cccagagcca
     6901 ccaccacctc cccctccacc ccctccaccc ccacttccgg cagcgccgcc tcagccggcg
     6961 tccacaccag ccatccccgc atcagcccca cccatcacct cacctacaat tgcaccggcc
     7021 cagccatcag tgccgctcac ccagctctcc atgccgatgg agctgcccat cttctcgccg
     7081 ctgatgatgc agacgatgcc gctgcagacc ttgccggctc agctaccccc gcagctggga
     7141 cctgtggagc ctctgcctgc ggacctggcc caactctacc agcatcagct caatccaacc
     7201 ctgctccagc agcagaacaa gaggcctcgc accaggatca cagatgatca gctccgagtc
     7261 ttgcggcaat attttgacat taacaactcc cccagtgaag agcaaataaa agagatggca
     7321 gacaagtccg ggttgcccca gaaagtgatc aagcactggt tcaggaacac tctcttcaaa
     7381 gagaggcagc gtaacaagga ctccccttac aacttcagta atcctcctat caccagcctg
     7441 gaggagctca agattgactc ccggccccct tcgccggaac ctccaaagca ggagtactgg
     7501 ggaagcaaga ggtcttcaag aacaaggttt acggactacc agctgagggt cttacaggac
     7561 ttcttcgatg ccaatgctta cccaaaggat gatgaatttg agcaactctc taatttactg
     7621 aaccttccaa cccgagtgat agtggtgtgg tttcagaatg cccgacagaa ggccaggaag
     7681 aattatgaga atcagggaga gggcaaagat ggagagcggc gtgagcttac aaacgataga
     7741 tacattcgaa caagcaactt gaactaccag tgcaaaaaat gtagcctggt gtttcagcgc
     7801 atctttgatc tcatcaagca ccagaagaag ctgtgttaca aggatgagga tgaggagggg
     7861 caggacgaca gccaaaatga ggattccatg gatgccatgg aaatcctgac gcctaccagc
     7921 tcatcctgca gtaccccgat gccctcacag gcttacagcg ccccagcacc atcagccaat
     7981 aatacagctt cctccgcttt cttgcagctt acagcggagg ctgaggaact ggccaccttc
     8041 aattcaaaaa cagaggcagg cgatgagaaa ccaaagctgg cggaagctcc cagtgcacag
     8101 ccaaaccaaa cccaagaaaa gcaaggacaa ccaaagccag agctgcagca gcaagagcag
     8161 cccgagcaga agaccaacac tccccagcag aagctccccc agctggtgtc cctgccttcg
     8221 ttgccacagc ctcctccaca agcgccccct ccacagtgcc ccttacccca gtcgagcccc
     8281 agtccttccc agctctccca cctgcccctc aagcccctcc acacatcaac tcctcaacag
     8341 ctcgcaaacc tacctcctca gctaatcccc taccagtgtg accagtgtaa gttggcattt
     8401 ccgtcatttg agcactggca ggagcatcag cagctccact tcctgagcgc gcagaaccag
     8461 ttcatccacc cccagttttt ggacaggtcc ctggatatgc ctttcatgct ctttgatccc
     8521 agtaacccac tcctggccag ccagctgctc tctggggcca tacctcagat tccagcaagc
     8581 tcagccactt ctccttcaac tccaacctcc acaatgaaca ctctcaagag gaagctggag
     8641 gaaaaggcca gtgcaagccc tggcgaaaac gacagtggga caggaggaga agagcctcag
     8701 agagacaagc gtttgagaac aaccatcaca ccggaacaac tagaaattct ctaccagaag
     8761 tatctactgg attccaatcc gactcgaaag atgttggatc acattgcaca cgaggtgggc
     8821 ttgaagaaac gtgtggtaca agtctggttt cagaacaccc gagctcggga aaggaaagga
     8881 cagttccggg ctgtaggccc agcgcaggcc cacaggagat gccctttttg cagagcgctc
     8941 ttcaaagcca agactgctct cgaggctcat atccggtccc gtcactggca tgaagccaag
     9001 agagctggct acaacctaac tctgtctgcg atgctcttag actgtgatgg gggactccag
     9061 atgaaaggag atatttttga cggaactagc ttttcccacc tacccccaag cagtagtgat
     9121 ggtcagggtg tccccctctc acctgtgagt aaaaccatgg aattgtcacc cagaactctt
     9181 ctaagccctt cctccattaa ggtggaaggg attgaagact ttgaaagccc ctccatgtcc
     9241 tcagttaatc taaactttga ccaaactaag ctggacaacg atgactgttc ctctgtcaac
     9301 acagcaatca cagataccac aactggagac gagggcaacg cagataacga cagtgcaacg
     9361 ggaatagcaa ctgaaaccaa atcctcttct gcacccaacg aagggttgac caaagcggcc
     9421 atgatggcaa tgtctgagta tgaagatcgg ttgtcatctg gtctggtcag cccggccccg
     9481 agcttttata gcaaggaata tgacaatgaa ggtacagtgg actacagtga aacctcaagc
     9541 cttgcagatc cctgctcccc gagtcctggt gcgagtggat ctgcaggcaa atctggtgac
     9601 agcggggatc ggcctgggca gaaacgtttt cgcactcaaa tgaccaatct gcagctgaag
     9661 gtcctcaagt catgctttaa tgactacagg acacccacta tgctagaatg tgaggtcctg
     9721 ggcaatgaca ttggactgcc aaagagagtc gttcaggtct ggttccagaa tgcccgggca
     9781 aaagaaaaga agtccaagtt aagcatggcc aagcattttg gtataaacca aacgagttat
     9841 gagggaccca aaacagagtg cactttgtgt ggcatcaagt acagcgctcg gctgtctgta
     9901 cgtgaccata tcttttccca acagcatatc tccaaagtta aagacaccat tggaagccag
     9961 ctggacaagg agaaagaata ctttgaccca gccaccgtac gtcagttgat ggctcaacaa
    10021 gagttggacc ggattaaaaa ggccaacgag gtccttggac tggcagctca gcagcaaggg
    10081 atgtttgaca acacccctct tcaggccctt aaccttccta cagcatatcc agcgctccag
    10141 ggcattcctc ctgtgttgct cccgggcctc aacagcccct ccttgccagg ctttactcca
    10201 tccaacacag ctttaacgtc tcctaagccg aacttgatgg gtctgcccag cacaactgtt
    10261 ccttcccctg gcctccccac ttctggatta ccaaataaac cgtcctcagc gtcgctgagc
    10321 tccccaaccc cagcacaagc cacgatggcg atgggccctc agcaaccccc ccagcagcag
    10381 cagcagcagc agcaaccaca ggtgcagcag cctcccccgc cgccagcagc ccagccgcca
    10441 cccacaccac agctcccact gcaacagcag cagcaacgca aggacaaaga cagtgagaaa
    10501 gtaaaggaga aggaaaaggc acacaaaggg aaaggggaac ccctgcctgt ccccaagaag
    10561 gagaaaggag aggcccccac ggcaactgca gccacgatct cagccccgct gcccaccatg
    10621 gagtatgcgg tagaccctgc acagctgcag gccctgcagg ccgcgttgac ttcggacccc
    10681 acagcattgc tcacaagcca gttccttcct tactttgtac caggcttttc tccttattat
    10741 gctccccaga tccctggcgc cctgcagagc gggtacctgc agcctatgta tggcatggaa
    10801 ggcctgttcc cctacagccc tgcactgtcg caggccctga tggggctgtc cccaggctcc
    10861 ctactgcagc agtaccagca ataccagcag agtctgcagg aggcaattca gcagcagcag
    10921 cagcggcaac tacagcagca gcagcagcaa aaagtgcagc agcagcagcc caaagcaagc
    10981 caaaccccag tcccccccgg ggctccttcc ccagacaaag accctgccaa agaatccccc
    11041 aaaccagaag aacagaaaaa caccccccgt gaggtgtccc ccctcctgcc gaaactccct
    11101 gaagagccag aagcagaaag caaaagtgcg gactccctct acgacccctt cattgttcca
    11161 aaggtgcagt acaagttggt ctgccgcaag tgccaggcgg gcttcagcga cgaggaggca
    11221 gcgaggagcc acctgaagtc cctctgcttc ttcggccagt ctgtggtgaa cctgcaagag
    11281 atggtgcttc acgtccccac cggcggcggc ggcggtggca gtggcggcgg cggcggcggt
    11341 ggcggcggcg gcggcggcgg cggctcgtac cactgcctgg cgtgcgagag cgcgctctgt
    11401 ggggaggaag ctctgagtca acatctcgag tcggccttgc acaaacacag aacaatcacg
    11461 agagcagcaa gaaacgccaa agagcaccct agtttattac ctcactctgc ctgcttcccc
    11521 gatcctagca ccgcatctac ctcgcagtct gccgctcact caaacgacag cccccctccc
    11581 ccgtcggccg ccgccccctc ctccgcttcc ccccacgcct ccaggaagtc ttggccgcaa
    11641 gtggtctccc gggcttcggc agcgaagccc ccttcttttc ctcctctctc ctcatcttca
    11701 acggttacct caagttcatg cagcacctca ggggttcagc cctcgatgcc aacagacgac
    11761 tattcggagg agtctgacac ggatctcagc caaaagtccg acggaccggc gagcccggtg
    11821 gagggtccca aagaccccag ctgccccaag gacagtggtc tgaccagtgt aggaacggac
    11881 accttcagat tgtaagcttt gaagatgaac aatacaaaca aatgaattta aatacaaaaa
    11941 ttaataacaa accaatttca aaaatagact aactgcaatt ccaaagcttc taaccaaaaa
    12001 acaaaaaaaa aaaaaaaaaa aactcgaggc atctatgtcg ggtgcggaga aagaggtaat
    12061 gaaatggcac atggtcatag ctgtttcctg acccagcttt cttgtacaaa gtggtagatc
    12121 ctctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
    12181 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    12241 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    12301 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    12361 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
    12421 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
    12481 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    12541 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    12601 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    12661 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    12721 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    12781 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    12841 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
    12901 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    12961 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    13021 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    13081 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    13141 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
    13201 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
    13261 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
    13321 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
    13381 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
    13441 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
    13501 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
    13561 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
    13621 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
    13681 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
    13741 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
    13801 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
    13861 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
    13921 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
    13981 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
    14041 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
    14101 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
    14161 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
    14221 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
    14281 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    14341 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
    14401 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    14461 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    14521 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    14581 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    14641 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    14701 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    14761 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    14821 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    14881 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    14941 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    15001 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    15061 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    15121 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    15181 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    15241 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    15301 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    15361 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    15421 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    15481 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    15541 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    15601 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    15661 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    15721 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    15781 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    15841 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    15901 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    15961 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    16021 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    16081 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    16141 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    16201 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    16261 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    16321 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    16381 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    16441 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    16501 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    16561 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
    16621 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
    16681 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
    16741 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
    16801 gggccagata tacgcgtt
//
