LOCUS       sequence-235867-        8926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression, Genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou
            Q, Li W
  TITLE     Enhanced mammalian genome editing by new Cas12a orthologs with 
            optimized crRNA scaffolds.
  JOURNAL   Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8.
  PUBMED    30717767
REFERENCE   2  (bases 1 to 8926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2019-02-5"; volume: "20(1):15. doi"; pages: " 
            10.1186/s13059-019-1620-8"
COMMENT     Sequence Label: pCAG-BsCas12a-2AeGFP
FEATURES             Location/Qualifiers
     source          1..8926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..665
                     /label=chicken beta-actin promoter
     intron          666..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1739..1757
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1769..1789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5420..5440
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5447..5536
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             5558..5611
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5612..5614,5615..5617,5618..6331)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5612..5614,5615..5617,5618..6328)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5657..5678)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5918..5937)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6265..6286
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6374..6495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6411..6430)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6465..6484
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6538..6554)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6538..6554)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6551..6573)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6562..6578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6586..6592,6593..6610,6611..6616))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6631..6652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6769..6786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6940..7528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7020..7039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7699..8490,8491..8559))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8322..8341
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8560..8664)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8743..8762)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      481 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      541 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      661 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      841 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgtcc
     1561 gcagggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagtgcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagcta gtgaattcta
     1741 atacgactca ctataggccg ccaccatgcc caagaagaag aggaaggttc ccgggatgta
     1801 ctaccagaac ctgaccaaga agtaccccgt gagcaagacc atccgcaacg agctgatccc
     1861 catcggcaag accctggaga acatccgcaa gaacaacatc ctggagagcg acgtgaagcg
     1921 caagcaggac tacgagcacg tgaagggcat catggacgag taccacaagc agctgatcaa
     1981 cgaggccctg gacaactaca tgctgcccag cctgaaccag gccgccgaga tctacctgaa
     2041 gaagcacgtg gacgtggagg accgcgagga gttcaagaag acccaggacc tgctgcgccg
     2101 cgaggtgacc ggccgcctga aggagcacga gaactacacc aagatcggca agaaggacat
     2161 cctggacctg ctggagaagc tgcccagcat cagcgaggag gactacaacg ccctggagag
     2221 cttccgcaac ttctacacct acttcaccag ctacaacaag gtgcgcgaga acctgtacag
     2281 cgacgaggag aagagcagca ccgtggccta ccgcctgatc aacgagaacc tgcccaagtt
     2341 cctggacaac atcaagagct acgccttcgt gaaggccgcc ggcgtgctgg ccgactgcat
     2401 cgaggaggag gagcaggacg ccctgttcat ggtggagacc ttcaacatga ccctgaccca
     2461 ggagggcatc gacatgtaca actaccagat cggcaaggtg aacagcgcca tcaacctgta
     2521 caaccagaag aaccacaagg tggaggagtt caagaagatc cccaagatga aggtgctgta
     2581 caagcagatc ctgagcgacc gcgaggaggt gttcatcggc gagttcaagg acgacgagac
     2641 cctgctgagc agcatcggcg cctacggcaa cgtgctgatg acctacctga agagcgagaa
     2701 gatcaacatc ttcttcgacg ccctgcgcga gagcgagggc aagaacgtgt acgtgaagaa
     2761 cgacctgagc aagaccacca tgagcaacat cgtgttcggc agctggagcg ccttcgacga
     2821 gctgctgaac caggagtacg acctggccaa cgagaacaag aagaaggacg acaagtactt
     2881 cgagaagcgc cagaaggagc tgaagaagaa caagagctac accctggagc agatgagcaa
     2941 cctgagcaag gaggacatca gccccatcga gaactacatc gagcgcatca gcgaggacat
     3001 cgagaagatc tgcatctaca acggcgagtt cgagaagatc gtggtgaacg agcacgacag
     3061 cagccgcaag ctgagcaaga acatcaaggc cgtgaaggtg atcaaggact acctggacag
     3121 catcaaggag ctggagcacg acatcaagct gatcaacggc agcggccagg agctggagaa
     3181 gaacctggtg gtgtacgtgg gccaggagga ggccctggag cagctgcgcc ccgtggacag
     3241 cctgtacaac ctgacccgca actacctgac caagaagccc ttcagcaccg agaaggtgaa
     3301 gctgaacttc aacaagagca ccctgctgaa cggctgggac aagaacaagg agaccgacaa
     3361 cctgggcatc ctgttcttca aggacggcaa gtactacctg ggcatcatga acaccaccgc
     3421 caacaaggcc ttcgtgaacc cccccgccgc caagaccgag aacgtgttca agaaggtgga
     3481 ctacaagctg ctgcccggca gcaacaagat gctgcccaag gtgttcttcg ccaagagcaa
     3541 catcggctac tacaacccca gcaccgagct gtacagcaac tacaagaagg gcacccacaa
     3601 gaagggcccc agcttcagca tcgacgactg ccacaacctg atcgacttct tcaaggagag
     3661 catcaagaag cacgaggact ggagcaagtt cggcttcgag ttcagcgaca ccgccgacta
     3721 ccgcgacatc agcgagttct accgcgaggt ggagaagcag ggctacaagc tgaccttcac
     3781 cgacatcgac gagagctaca tcaacgacct gatcgagaag aacgagctgt acctgttcca
     3841 gatctacaac aaggacttca gcgagtacag caagggcaag ctgaacctgc acaccctgta
     3901 cttcatgatg ctgttcgacc agcgcaacct ggacaacgtg gtgtacaagc tgaacggcga
     3961 ggccgaggtg ttctaccgcc ccgccagcat cgccgagaac gagctggtga tccacaaggc
     4021 cggcgagggc atcaagaaca agaaccccaa ccgcgccaag gtgaaggaga ccagcacctt
     4081 cagctacgac atcgtgaagg acaagcgcta cagcaagtac aagttcaccc tgcacatccc
     4141 catcaccatg aacttcggcg tggacgaggt gcgccgcttc aacgacgtga tcaacaacgc
     4201 cctgcgcacc gacgacaacg tgaacgtgat cggcatcgac cgcggcgagc gcaacctgct
     4261 gtacgtggtg gtgatcaaca gcgagggcaa gatcctggag cagatcagcc tgaacagcat
     4321 catcaacaag gagtacgaca tcgagaccaa ctaccacgcc ctgctggacg agcgcgagga
     4381 cgaccgcaac aaggcccgca aggactggaa caccatcgag aacatcaagg agctgaagac
     4441 cggctacctg agccaggtgg tgaacgtggt ggccaagctg gtgctgaagt acaacgccat
     4501 catctgcctg gaggacctga acttcggctt caagcgcggc cgccagaagg tggagaagca
     4561 ggtgtaccag aagttcgaga agatgctgat cgagaagctg aactacctgg tgatcgacaa
     4621 gagccgcgag caggtgagcc ccgagaagat gggcggcgcc ctgaacgccc tgcagctgac
     4681 cagcaagttc aagagcttcg ccgagctggg caagcagagc ggcatcatct actacgtgcc
     4741 cgcctacctg accagcaaga tcgaccccac caccggcttc gtgaacctgt tctacatcaa
     4801 gtacgagaac atcgagaagg ccaagcagtt cttcgacggc ttcgacttca tccgcttcaa
     4861 caagaaggac gacatgttcg agttcagctt cgactacaag agcttcaccc agaaggcctg
     4921 cggcatccgc agcaagtgga tcgtgtacac caacggcgag cgcatcatca agtaccccaa
     4981 ccccgagaag aacaacctgt tcgacgagaa ggtgatcaac gtgaccgacg agatcaaggg
     5041 cctgttcaag cagtaccgca tcccctacga gaacggcgag gacatcaagg agatcatcat
     5101 cagcaaggcc gaggccgact tctacaagcg cctgttccgc ctgctgcacc agaccctgca
     5161 gatgcgcaac agcaccagcg acggcacccg cgactacatc atcagccccg tgaagaacga
     5221 ccgcggcgag ttcttctgca gcgagttcag cgagggcacc atgcccaagg acgccgacgc
     5281 caacggcgcc tacaacatcg cccgcaaggg cctgtgggtg ctggagcaga tccgccagaa
     5341 ggacgagggc gagaaggtga acctgagcat gaccaacgcc gagtggctga agtacgccca
     5401 gctgcacctg ctggctagcc caaagaagaa gaggaaagtc tctagatacc cttatgatgt
     5461 tccagattat gccggatacc catacgatgt ccctgactat gcaggctcct acccttatga
     5521 cgtcccagac tacgccggat ccaggtccgg cggcggagag ggcagaggaa gtcttctaac
     5581 atgcggtgac gtggaggaga atcccggccc aatggtgagc aagggcgagg agctgttcac
     5641 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     5701 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     5761 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     5821 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5881 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5941 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     6001 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     6061 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     6121 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     6181 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     6241 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     6301 cactctcggc atggacgagc tgtacaagta actgcagcgc ggggatctca tgctggagtt
     6361 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6421 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6481 catcaatgta tcttattaat taacgtcgac ctctagctag agcttggcgt aatcatggtc
     6541 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6601 aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt
     6661 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6721 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6781 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6841 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6901 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6961 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7021 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7081 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7141 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7201 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7261 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7321 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7381 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7441 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7501 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7561 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7621 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7681 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7741 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7801 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7861 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7921 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7981 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8041 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8101 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8161 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8221 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8281 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8341 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8401 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8461 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8521 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8581 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8641 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     8701 atcgggagat cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc
     8761 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     8821 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     8881 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatat
//
