LOCUS       sequence-235868-        9238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression, Genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou
            Q, Li W
  TITLE     Enhanced mammalian genome editing by new Cas12a orthologs with 
            optimized crRNA scaffolds.
  JOURNAL   Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8.
  PUBMED    30717767
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2019-02-5"; volume: "20(1):15. doi"; pages: " 
            10.1186/s13059-019-1620-8"
COMMENT     Sequence Label: pCAG-HkCas12a-2AeGFP
FEATURES             Location/Qualifiers
     source          1..9238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..665
                     /label=chicken beta-actin promoter
     intron          666..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1739..1757
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1769..1789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5732..5752
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5759..5848
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             5870..5923
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5924..5926,5927..5929,5930..6643)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5924..5926,5927..5929,5930..6640)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5969..5990)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6230..6249)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6577..6598
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6686..6807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6723..6742)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6777..6796
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6850..6866)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6850..6866)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6863..6885)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6874..6890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6898..6904,6905..6922,6923..6928))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6943..6964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7081..7098)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7252..7840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7332..7351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8011..8802,8803..8871))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8634..8653
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8872..8976)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9055..9074)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      481 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      541 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      661 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      841 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgtcc
     1561 gcagggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagcgcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagcta gtgaattcta
     1741 atacgactca ctataggccg ccaccatgcc caagaagaag aggaaggttc ccgggatgtt
     1801 cgagaagctg agcaacatcg tgagcatcag caagaccatc cgcttcaagc tgatccccgt
     1861 gggcaagacc ctggagaaca tcgagaagct gggcaagctg gagaaggact tcgagcgcag
     1921 cgacttctac cccatcctga agaacatcag cgacgactac taccgccagt acatcaagga
     1981 gaagctgagc gacctgaacc tggactggca gaagctgtac gacgcccacg agctgctgga
     2041 cagcagcaag aaggagagcc agaagaacct ggagatgatc caggcccagt accgcaaggt
     2101 gctgttcaac atcctgagcg gcgagctgga caagagcggc gagaagaaca gcaaggacct
     2161 gatcaagaac aacaaggccc tgtacggcaa gctgttcaag aagcagttca tcctggaggt
     2221 gctgcccgac ttcgtgaaca acaacgacag ctacagcgag gaggacctgg agggcctgaa
     2281 cctgtacagc aagttcacca cccgcctgaa gaacttctgg gagacccgca agaacgtgtt
     2341 caccgacaag gacatcgtga ccgccatccc cttccgcgcc gtgaacgaga acttcggctt
     2401 ctactacgac aacatcaaga tcttcaacaa gaacatcgag tacctggaga acaagatccc
     2461 caacctggag aacgagctga aggaggccga catcctggac gacaaccgca gcgtgaagga
     2521 ctacttcacc cccaacggct tcaactacgt gatcacccag gacggcatcg acgtgtacca
     2581 ggccatccgc ggcggcttca ccaaggagaa cggcgagaag gtgcagggca tcaacgagat
     2641 cctgaacctg acccagcagc agctgcgccg caagcccgag accaagaacg tgaagctggg
     2701 cgtgctgacc aagctgcgca agcagatcct ggagtacagc gagagcacca gcttcctgat
     2761 cgaccagatc gaggacgaca acgacctggt ggaccgcatc aacaagttca acgtgagctt
     2821 cttcgagagc accgaggtga gccccagcct gttcgagcag atcgagcgcc tgtacaacgc
     2881 cctgaagagc atcaagaagg aggaggtgta catcgacgcc cgcaacaccc agaagttcag
     2941 ccagatgctg ttcggccagt gggacgtgat ccgccgcggc tacaccgtga agatcaccga
     3001 gggcagcaag gaggagaaga agaagtacaa ggagtacctg gagctggacg agaccagcaa
     3061 ggccaagcgc tacctgaaca tccgcgagat cgaggagctg gtgaacctgg tggagggctt
     3121 cgaggaggtg gacgtgttca gcgtgctgct ggagaagttc aagatgaaca acatcgagcg
     3181 cagcgagttc gaggccccca tctacggcag ccccatcaag ctggaggcca tcaaggagta
     3241 cctggagaag cacctggagg agtaccacaa gtggaagctg ctgctgatcg gcaacgacga
     3301 cctggacacc gacgagacct tctaccccct gctgaacgag gtgatcagcg actactacat
     3361 catccccctg tacaacctga cccgcaacta cctgacccgc aagcacagcg acaaggacaa
     3421 gatcaaggtg aacttcgact tccccaccct ggccgacggc tggagcgaga gcaagatcag
     3481 cgacaaccgc agcatcatcc tgcgcaaggg cggctactac tacctgggca tcctgatcga
     3541 caacaagctg ctgatcaaca agaagaacaa gagcaagaag atctacgaga tcctgatcta
     3601 caaccagatc cccgagttca gcaagagcat ccccaactac cccttcacca agaaggtgaa
     3661 ggagcacttc aagaacaacg tgagcgactt ccagctgatc gacggctacg tgagccccct
     3721 gatcatcacc aaggagatct acgacatcaa gaaggagaag aagtacaaga aggacttcta
     3781 caaggacaac aacaccaaca agaactacct gtacaccatc tacaagtgga tcgagttctg
     3841 caagcagttc ctgtacaagt acaagggccc caacaaggag agctacaagg agatgtacga
     3901 cttcagcacc ctgaaggaca ccagcctgta cgtgaacctg aacgacttct acgccgacgt
     3961 gaacagctgc gcctaccgcg tgctgttcaa caagatcgac gagaacacca tcgacaacgc
     4021 cgtggaggac ggcaagctgc tgctgttcca gatctacaac aaggacttca gccccgagag
     4081 caagggcaag aagaacctgc acaccctgta ctggctgagc atgttcagcg aggagaacct
     4141 gcgcacccgc aagctgaagc tgaacggcca ggccgagatc ttctaccgca agaagctgga
     4201 gaagaagccc atcatccaca aggagggcag catcctgctg aacaagatcg acaaggaggg
     4261 caacaccatc cccgagaaca tctaccacga gtgctaccgc tacctgaaca agaagatcgg
     4321 ccgcgaggac ctgagcgacg aggccatcgc cctgttcaac aaggacgtgc tgaagtacaa
     4381 ggaggcccgc ttcgacatca tcaaggaccg ccgctacagc gagagccagt tcttcttcca
     4441 cgtgcccatc accttcaact gggacatcaa gaccaacaag aacgtgaacc agatcgtgca
     4501 gggcatgatc aaggacggcg agatcaagca catcatcggc atcgaccgcg gcgagcgcca
     4561 cctgctgtac tacagcgtga tcgacctgga gggcaacatc gtggagcagg gcagcctgaa
     4621 caccctggag cagaaccgct tcgacaacag caccgtgaag gtggactacc agaacaagct
     4681 gcgcacccgc gaggaggacc gcgaccgcgc ccgcaagaac tggaccaaca tcaacaagat
     4741 caaggagctg aaggacggct acctgagcca cgtggtgcac aagctgagcc gcctgatcat
     4801 caagtacgag gccatcgtga tcatggagaa cctgaaccag ggcttcaagc gcggccgctt
     4861 caaggtggag cgccaggtgt accagaagtt cgagctggcc ctgatgaaca agctgagcgc
     4921 cctgagcttc aaggagaagt acgacgagcg caagaacctg gagcccagcg gcatcctgaa
     4981 ccccatccag gcctgctacc ccgtggacgc ctaccaggag ctgcagggcc agaacggcat
     5041 cgtgttctac ctgcccgccg cctacaccag cgtgatcgac cccgtgaccg gcttcaccaa
     5101 cctgttccgc ctgaagagca tcaacagcag caagtacgag gagttcatca agaagttcaa
     5161 gaacatctac ttcgacaacg aggaggagga cttcaagttc atcttcaact acaaggactt
     5221 cgccaaggcc aacctggtga tcctgaacaa catcaagagc aaggactgga agatcagcac
     5281 ccgcggcgag cgcatcagct acaacagcaa gaagaaggag tacttctacg tgcagcccac
     5341 cgagttcctg atcaacaagc tgaaggagct gaacatcgac tacgagaaca tcgacatcat
     5401 ccccctgatc gacaacctgg aggagaaggc caagcgcaag atcctgaagg ccctgttcga
     5461 caccttcaag tacagcgtgc agctgcgcaa ctacgacttc gagaacgact acatcatcag
     5521 ccccaccgcc gacgacaacg gcaactacta caacagcaac gagatcgaca tcgacaagac
     5581 caacctgccc aacaacggcg acgccaacgg cgccttcaac atcgcccgca agggcctgct
     5641 gctgaaggac cgcatcgtga acagcaacga gagcaaggtg gacctgaaga tcaagaacga
     5701 ggactggatc aacttcatca tcagcgctag cccaaagaag aagaggaaag tctctagata
     5761 cccttatgat gttccagatt atgccggata cccatacgat gtccctgact atgcaggctc
     5821 ctacccttat gacgtcccag actacgccgg atccaggtcc ggcggcggag agggcagagg
     5881 aagtcttcta acatgcggtg acgtggagga gaatcccggc ccaatggtga gcaagggcga
     5941 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     6001 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     6061 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     6121 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     6181 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     6241 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     6301 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     6361 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     6421 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     6481 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     6541 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     6601 cgccgccggg atcactctcg gcatggacga gctgtacaag taactgcagc gcggggatct
     6661 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     6721 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6781 tttgtccaaa ctcatcaatg tatcttatta attaacgtcg acctctagct agagcttggc
     6841 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6901 catacgagcc ggaagcataa agtgtaaagc ctagggtgcc taatgagtga gctaactcac
     6961 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7021 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7081 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7141 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7201 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7261 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7321 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7381 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7441 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7501 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7561 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7621 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7681 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7741 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7801 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7861 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7921 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7981 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8041 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8101 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8161 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8221 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8281 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8341 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8401 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8461 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8521 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8581 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8641 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8701 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8761 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8821 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8881 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8941 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9001 tgacgtcgac ggatcgggag atcgatctcc cgatccccta gggtcgactc tcagtacaat
     9061 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     9121 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     9181 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatat
//
