LOCUS       sequence-235869-        9226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression, Genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou
            Q, Li W
  TITLE     Enhanced mammalian genome editing by new Cas12a orthologs with 
            optimized crRNA scaffolds.
  JOURNAL   Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8.
  PUBMED    30717767
REFERENCE   2  (bases 1 to 9226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2019-02-5"; volume: "20(1):15. doi"; pages: " 
            10.1186/s13059-019-1620-8"
COMMENT     Sequence Label: pCAG-LpCas12a-2AeGFP
FEATURES             Location/Qualifiers
     source          1..9226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..665
                     /label=chicken beta-actin promoter
     intron          666..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1739..1757
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1769..1789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5720..5740
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5747..5836
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             5858..5911
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5912..5914,5915..5917,5918..6631)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5912..5914,5915..5917,5918..6628)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5957..5978)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6218..6237)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6565..6586
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6674..6795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6711..6730)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6765..6784
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6838..6854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6838..6854)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6851..6873)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6862..6878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6886..6892,6893..6910,6911..6916))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6931..6952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7069..7086)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7240..7828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7320..7339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7999..8790,8791..8859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8622..8641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8860..8964)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9043..9062)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      481 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      541 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      661 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      841 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgtcc
     1561 gcagggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagcgcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagcta gtgaattcta
     1741 atacgactca ctataggccg ccaccatgcc caagaagaag aggaaggttc ccgggatgat
     1801 catgaacaac gtgaccggcg acttcagcga gttcgtggcc atcagcaagg tgcagaagac
     1861 cctgcgcaac gagctgcgcc ccacccccct gaccatgaag cacatcaagc agaagggcat
     1921 catcaccgag gacgagtaca agacccagca gagcctggag ctgaagcgca tcgccgacgg
     1981 ctactaccgc gactacatca cccacaagct gaacgacacc aacaacctgg acttccgcaa
     2041 cctgttcgag gccatcgagg agaagtacaa gaagaacgac aaggacaacc gcgacaagct
     2101 ggacctggtg gagaagagca agcgcggcga gatcgccaag ctgctgagcg ccgacgacaa
     2161 cttcaagagc atgttcgagg ccaagctgat cacccagctg ctgcccgtgt acgtggagca
     2221 gaactacatc ggcgaggaca aggagaaggc cctggagacc atcgccctgt tcaagggctt
     2281 caccacctac ttcaccgact acttcaacat ccgcaagaac atgttcaagg agaacggcgg
     2341 cgccagcagc atctgctacc gcatcgtgaa cgtgaacgcc agcatcttct acgacaacct
     2401 gaagaccttc atgtgcatca aggagaaggc cgagaccgag atcgccctga tcgaggagga
     2461 gctgaccgag ctgctggaca gctggcgcct ggagcacatc ttcagcgagg actactacaa
     2521 cgagctgctg gcccagaagg gcatcgacta ctacaaccag atctgcggcg acgtgaacaa
     2581 gcacatgaac ctgtactgcc agcagaacaa gctgaaggcc aacgtgttca agatgaccaa
     2641 gctgcagaag cagatcatgg gcatcagcga gaaggccttc gagatccccc ccatgtacca
     2701 gaacgacgag gaggtgtacg ccgccttcaa cggcttcatc agccgcctgg aggaggtgaa
     2761 gctgatcgac cgcctgggca acgtgctgca gaacagcaac atctacgaca ccgccaagat
     2821 ctacatcaac gcccgctgct acaccaacgt gagcagctac gtgtacggcg gctggggcgt
     2881 gatcgagagc gccatcgagc gctactggta caacaccatc gccggcaagg gccagagcaa
     2941 ggccaagaag atcgagaagg ccaagaagga caacaagttc atgagcgtga aggagctgga
     3001 cagcatcgtg agcgactacg agcccgacta cttcaacgcc agcaacatgg acgacgacaa
     3061 cagcggccgc gccttcagcg gccacggcgt gctgggctac ttcaacaaga tgagcaagct
     3121 gctggccaac atgagcctgc acaccatcac ctacgacagc ggcgacagcc tgatcgagaa
     3181 caaggagacc gccctgaaca tcaagaagga cctggacgac atcatgagca tctaccactg
     3241 gctgcagacc ttcatcatcg acgaggtggt ggagaaggac aacgccttct acgccgagct
     3301 ggaggacatc tactacgagc tggagaacgt ggtgaccctg tacgaccgca tccgcaacta
     3361 cgtgacccgc aagccctaca gcacccagaa gttcaagctt aacttcgcca gccccaccct
     3421 ggccagcggc tggagccgca gcaaggagtt cgacaacaac gccatcatcc tgctgcgcaa
     3481 caacaagtac tacatcgcca tcttcaacgt gaacaacaag cccgacaagc agatcatcaa
     3541 gggcagcgag gagcagcgcc tgagcaccga ctacaagaag atggtgtaca acctgctgcc
     3601 cggccccaac aagatgctgc cctgggtgtt catcaagagc aacaccggca agcgcgacta
     3661 caaccccagc agctacatcc tggagggcta cgagaagaac cgccacatca agagcagcgg
     3721 caacttcgac atcaactact gccacgacct gatcgactac tacaaggcct gcatcaacaa
     3781 gcaccccgag tggaagaact acggcttcaa gttcaaggag accacccagt acaacgacat
     3841 cggccagttc tacaaggacg tggagaagca gggctacagc atcagctggg cctacatcag
     3901 cgaggccgac atcaaccgcc tggacgagga gggcaagatc tacctgttcg agatctacaa
     3961 caaggacctg agcagccaca gcaccggcaa ggacaacctg cacaccatgt acctgaagaa
     4021 catcttcagc gaggacaacc tgaagaacat ctgcatcgag ctgaacggca acgccgagct
     4081 gttctaccgc aagagcagca tgaagcgcaa catcacccac aagaaggaca ccgtgctggt
     4141 gaacaagacc tacatcaacg aggccggcgt gcgcgtgagc ctgaccgacg aggactacat
     4201 caaggtgtac aactactaca acaacgacta cgtgatcgac gtggagaagg acaagaagct
     4261 ggtggagatc ctggagcgca tcggccaccg caagaacccc atcgacatca tcaaggacaa
     4321 gcgctacacc gaggacaagt acttcctgca cttccccatc accatcaact acggcgtgga
     4381 cgacgagaac atcaacgcca agatgatcga gtacatcgcc aagcacaaca acatgaacgt
     4441 gatcggcatc gaccgcggcg agcgcaacct gatctacatc agcgtgatca acaacaaggg
     4501 caacatcatc gagcagaaga gcttcaacct ggtgaacaac tacgactaca agaacaagct
     4561 gaagaacatg gagaagaccc gcgacaacgc ccgcaagaac tggcaggaga tcggcaagat
     4621 caaggacgtg aagaacggct acctgagcgg cgtgatcagc aagatcgccc gcatggtggt
     4681 ggactacaac gccatcatcg tgatggagga cctgaaccgc ggcttcaagc gcggccgctt
     4741 caaggtggag cgccaggtgt accagaagtt cgagaacatg ctgatcagca agctgaacta
     4801 cctggtgttc aaggagaaga aggccgacga gaacggcggc atcctgaagg gctaccagct
     4861 gacctacctg cccaagagcg ccctgcagat cggcaagcag tgcggctgca tcttctacgt
     4921 gcccgccgcc tacaccagca agatcgaccc cgccaccggc ttcatcaaca tcttcgactt
     4981 caagaagtac agcggcagcg ccatcaacgc caaggtgaag gacaagaagg agttcctgat
     5041 gagcatgaac agcatccgct acgtgaacga gggcagcgcc gagtacgaga agatcggcca
     5101 ccgccagctg ttcgccttca gcttcgacta caacaacttc aagacctaca acgtgagcat
     5161 ccccgtgaac gagtggacca cctacaccta cggcgagcgc atcaagaagc tgtacaagga
     5221 cggccgctgg agcggcagcg aggtgctgaa cctgaccgag gacctgatcg agctgatgga
     5281 gcagtacggc atcgagtaca aggacggcca cgacatccgc gaggacatca gccacatgga
     5341 cgagatgcgc aacgccgact tcatctgcaa cctgttcgag aagttcaagt acaccgtgca
     5401 gctgcgcaac agcaagagcg aggccgaggg cgacgactac gaccgcctgg tgagccccgt
     5461 gctgaacagc cacaacggct tcttcgacag cagcgactac aaggagaacg agaagagcga
     5521 cgacatcatc gacgacaagc agatcatgcc caaggacgcc gacgccaacg gcgcctactg
     5581 catcgccctg aagggcctgt acgagatcaa caagatcaag gagaactgga gcgacgacaa
     5641 gaagctgaag gagagcgagc tgtacatcgg cgtgaccgag tggctggact acatccagaa
     5701 ccgccgcttc gaggctagcc caaagaagaa gaggaaagtc tctagatacc cttatgatgt
     5761 tccagattat gccggatacc catacgatgt ccctgactat gcaggctcct acccttatga
     5821 cgtcccagac tacgccggat ccaggtccgg cggcggagag ggcagaggaa gtcttctaac
     5881 atgcggtgac gtggaggaga atcccggccc aatggtgagc aagggcgagg agctgttcac
     5941 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     6001 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     6061 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     6121 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     6181 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     6241 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     6301 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     6361 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     6421 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     6481 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     6541 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     6601 cactctcggc atggacgagc tgtacaagta actgcagcgc ggggatctca tgctggagtt
     6661 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6721 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6781 catcaatgta tcttattaat taacgtcgac ctctagctag agcttggcgt aatcatggtc
     6841 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6901 aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt
     6961 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7021 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7081 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7141 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7201 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7261 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7321 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7381 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7441 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7501 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7561 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7621 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7681 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7741 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7801 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7861 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7921 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7981 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8041 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8101 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8161 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8221 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8281 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8341 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8401 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8461 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8521 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8581 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8641 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8701 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8761 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8821 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8881 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8941 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     9001 atcgggagat cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc
     9061 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     9121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     9181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatat
//
