LOCUS       sequence-235870-        8947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression, Genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou
            Q, Li W
  TITLE     Enhanced mammalian genome editing by new Cas12a orthologs with 
            optimized crRNA scaffolds.
  JOURNAL   Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8.
  PUBMED    30717767
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2019-02-5"; volume: "20(1):15. doi"; pages: " 
            10.1186/s13059-019-1620-8"
COMMENT     Sequence Label: pCAG-PrCas12a-2AeGFP
FEATURES             Location/Qualifiers
     source          1..8947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..665
                     /label=chicken beta-actin promoter
     intron          666..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1739..1757
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1769..1789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5441..5461
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5468..5557
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             5579..5632
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5633..5635,5636..5638,5639..6352)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5633..5635,5636..5638,5639..6349)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5678..5699)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5939..5958)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6286..6307
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6395..6516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6432..6451)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6486..6505
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6559..6575)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6559..6575)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6572..6594)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6583..6599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6607..6613,6614..6631,6632..6637))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6652..6673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6790..6807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6961..7549)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7041..7060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7720..8511,8512..8580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8343..8362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8581..8685)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8764..8783)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      481 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      541 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      661 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      841 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgtcc
     1561 gcagggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagcgcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagcta gtgaattcta
     1741 atacgactca ctataggccg ccaccatgcc caagaagaag aggaaggttc ccgggatgat
     1801 catcggccgc gacttcaaca tgtactacca gaacctgacc aagatgtacc ccatcagcaa
     1861 gaccctgcgc aacgagctga tccccgtggg caagaccctg gagaacatcc gcaagaacgg
     1921 catcctggag gccgacatcc agcgcaaggc cgactacgag cacgtgaaga agctgatgga
     1981 caactaccac aagcagctga tcaacgaggc cctgcagggc gtgcacctga gcgacctgag
     2041 cgacgcctac gacctgtact tcaacctgag caaggagaag aacagcgtgg acgccttcag
     2101 caagtgccag gacaagctgc gcaaggagat cgtgagcctg ctgaagaacc acgagaactt
     2161 ccccaagatc ggcaacaagg agatcatcaa gctgctgcag agcctgtacg acaacgacac
     2221 cgactacaag gccctggaca gcttcagcaa cttctacacc tacttcagca gctacaacga
     2281 ggtgcgcaag aacctgtaca gcgacgagga gaagagcagc accgtggcct accgcctgat
     2341 caacgagaac ctgcccaagt tcctggacaa catcaaggcc tacgccatcg ccaagaaggc
     2401 cggcgtgcgc gccgagggcc tgagcgagga ggaccaggac tgcctgttca tcatcgagac
     2461 cttcgagcgc accctgaccc aggacggcat cgacaactac aacgccgcca tcggcaagct
     2521 gaacaccgcc atcaacctgt tcaaccagca gaacaagaag caggagggct tccgcaaggt
     2581 gccccagatg aagtgcctgt acaagcagat cctgagcgac cgcgaggagg ccttcatcga
     2641 cgagttcagc gacgacgagg acctgatcac caacatcgag agcttcgccg agaacatgaa
     2701 cgtgttcctg aacagcgaga tcatcaccga cttcaagatc gccctggtgg agagcgacgg
     2761 cagcctggtg tacatcaaga acgacgtgag caagaccagc ttcagcaaca tcgtgttcgg
     2821 cagctggaac gccatcgacg agaagctgag cgacgagtac gacctggcca acagcaagaa
     2881 gaagaaggac gagaagtact acgagaagcg ccagaaggag ctgaagaaga acaagagcta
     2941 cgacctggag accatcatcg gcctgttcga cgacaacagc gacgtgatcg gcaagtacat
     3001 cgagaagctg gagagcgaca tcaccgccat cgccgaggcc aagaacgact tcgacgagat
     3061 cgtgctgcgc aagcacgaca agaacaagag cctgcgcaag aacaccaacg ccgtggaggc
     3121 catcaagagc tacctggaca ccgtgaagga cttcgagcgc gacatcaagc tgatcaacgg
     3181 cagcggccag gaggtggaga agaacctggt ggtgtacgcc gagcaggaga acatcctggc
     3241 cgagatcaag aacgtggaca gcctgtacaa catgagccgc aactacctga cccagaagcc
     3301 cttcagcacc gagaagttca agctgaactt caaccgcgcc accctgctga acggctggga
     3361 caagaacaag gagaccgaca acctgggcat cctgttcgag aaggacggca tgtactacct
     3421 gggcatcatg aacaccaagg ccaacaagat cttcgtgaac atccccaagg ccaccagcaa
     3481 cgacgtgtac cacaaggtga actacaagct gctgcccggc cccaacaaga tgctgcccaa
     3541 ggtgttcttc gcccagagca acctggacta ctacaagccc agcgaggagc tgctggccaa
     3601 gtacaaggcc ggcacccaca agaagggcga caacttcagc ctggaggact gccacgccct
     3661 gatcgacttc ttcaaggcca gcatcgagaa gcaccccgac tggagcagct tcggcttcga
     3721 gttcagcgag acctgcacct acgaggacct gagcggcttc taccgcgagg tggagaagca
     3781 gggctacaag atcacctaca ccgacgtgga cgccgactac atcaccagcc tggtggagcg
     3841 cgacgagctg tacctgttcc agatctacaa caaggacttc agcccctaca gcaagggcaa
     3901 cctgaacctg cacaccatct acctgcagat gctgttcgac cagcgcaacc tgaacaacgt
     3961 ggtgtacaag ctgaacggcg aggccgaggt gttctaccgc cccgccagca tcaacgacga
     4021 ggaggtgatc atccacaagg ccggcgagga gatcaagaac aagaacagca agcgcgccgt
     4081 ggacaagccc accagcaagt tcggctacga catcatcaag gaccgccgct acagcaagga
     4141 caagttcatg ctgcacatcc ccgtgaccat gaacttcggc gtggacgaga cccgccgctt
     4201 caacgacgtg gtgaacgacg ccctgcgcaa cgacgagaag gtgcgcgtga tcggcatcga
     4261 ccgcggcgag cgcaacctgc tgtacgtggt ggtggtggac accgacggca ccatcctgga
     4321 gcagatcagc ctgaacagca tcatcaacaa cgagtacagc atcgagaccg actaccacaa
     4381 gctgctggac gagaaggagg gcgaccgcga ccgcgcccgc aagaactgga ccaccatcga
     4441 gaacatcaag gagctgaagg agggctacct gagccaggtg gtgaacgtga tcgccaagct
     4501 ggtgctgaag tacaacgcca tcatctgcct ggaggacctg aacttcggct tcaagcgcgg
     4561 ccgccagaag gtggagaagc aggtgtacca gaagttcgag aagatgctga tcgacaagct
     4621 gaactacctg gtgatcgaca agagccgcaa gcaggacaag cccgaggagt tcggcggcgc
     4681 cctgaacgcc ctgcagctga ccagcaagtt caccagcttc aaggacatgg gcaagcagac
     4741 cggcatcatc tactacgtgc ccgcctacct gaccagcaag atcgacccca ccaccggctt
     4801 cgccaacctg ttctacgtga agtacgagaa cgtggagaag gccaaggagt tcttcagccg
     4861 cttcgacagc atcagctaca acaacgagag cggctacttc gagttcgcct tcgactacaa
     4921 gaagttcacc gaccgcgcct gcggcgcccg cagccagtgg accgtgtgca cctacggcga
     4981 gcgcatcatc aagttccgca acaccgagaa gaacaacagc ttcgacgaca agaccatcgt
     5041 gctgagcgag gagttcaagg agctgttcag catctacggc atcagctacg aggacggcgc
     5101 cgagctgaag aacaagatca tgagcgtgga cgaggccgac ttcttccgca gcctgacccg
     5161 cctgttccag cagaccatgc agatgcgcaa cagcagcaac gacgtgaccc gcgactacat
     5221 catcagcccc atcatgaacg accgcggcga gttcttcaac agcgaggcct gcgacgccag
     5281 caagcccaag gacgccgacg ccaacggcgc cttcaacatc gcccgcaagg gcctgtgggt
     5341 gctggagcag atccgcaaca cccccagcgg cgacaagctg aacctggcca tgagcaacgc
     5401 cgagtggctg gagtacgccc agcgcaacca gatcgctagc ccaaagaaga agaggaaagt
     5461 ctctagatac ccttatgatg ttccagatta tgccggatac ccatacgatg tccctgacta
     5521 tgcaggctcc tacccttatg acgtcccaga ctacgccgga tccaggtccg gcggcggaga
     5581 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc caatggtgag
     5641 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5701 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5761 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     5821 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     5881 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     5941 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     6001 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     6061 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     6121 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     6181 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     6241 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     6301 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aactgcagcg
     6361 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     6421 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6481 tagttgtggt ttgtccaaac tcatcaatgt atcttattaa ttaacgtcga cctctagcta
     6541 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6601 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag
     6661 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6721 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6781 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6841 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6961 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7021 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7081 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7141 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7201 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7261 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7321 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7381 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7441 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7501 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7561 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7621 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7681 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7741 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7801 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7861 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7921 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7981 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8041 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8101 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8161 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8221 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8281 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8341 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8401 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8461 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8521 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8581 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8641 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8701 agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag ggtcgactct
     8761 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     8821 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     8881 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     8941 cagatat
//
