LOCUS       sequence-235871-        8953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression, Genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou
            Q, Li W
  TITLE     Enhanced mammalian genome editing by new Cas12a orthologs with 
            optimized crRNA scaffolds.
  JOURNAL   Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8.
  PUBMED    30717767
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2019-02-5"; volume: "20(1):15. doi"; pages: " 
            10.1186/s13059-019-1620-8"
COMMENT     Sequence Label: pCAG-PxCas12a-2AeGFP
FEATURES             Location/Qualifiers
     source          1..8953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..665
                     /label=chicken beta-actin promoter
     intron          666..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1739..1757
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1769..1789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5447..5467
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5474..5563
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             5585..5638
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5639..5641,5642..5644,5645..6358)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5639..5641,5642..5644,5645..6355)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5684..5705)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5945..5964)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6292..6313
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6401..6522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6438..6457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6492..6511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6565..6581)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6565..6581)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6578..6600)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6589..6605
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6613..6619,6620..6637,6638..6643))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6658..6679
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6796..6813)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6967..7555)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7047..7066)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7726..8517,8518..8586))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8349..8368
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8587..8691)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8770..8789)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      481 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      541 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      661 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      841 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgtcc
     1561 gcagggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagcgcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagcta gtgaattcta
     1741 atacgactca ctataggccg ccaccatgcc caagaagaag aggaaggttc ccgggatgat
     1801 catcggccgc gacttcaaca tgtactacca gaacctgacc aagatgtacc ccatcagcaa
     1861 gaccctgcgc aacgagctga tccccgtggg caagaccctg gagaacatcc gcaagaacgg
     1921 catcctggag gccgacatcc agcgcaaggc cgactacgag cacgtgaaga agctgatgga
     1981 caactaccac aagcagctga tcaacgaggc cctgcagggc gtgcacctga gcgacctgag
     2041 cgacgcctac gacctgtact tcaacctgag caaggagaag aacagcgtgg acgccttcag
     2101 caagtgccag gacaagctgc gcaaggagat cgtgagcttc ctgaagaacc acgagaactt
     2161 ccccaagatc ggcaacaagg agatcatcaa gctgatccag agcctgaacg acaacgacgc
     2221 cgacaacaac gccctggaca gcttcagcaa cttctacacc tacttcagca gctacaacga
     2281 ggtgcgcaag aacctgtaca gcgacgagga gaagagcagc accgtggcct accgcctgat
     2341 caacgagaac ctgcccaaga gcctggacaa catcaaggcc tacgccatcg ccaagaaggc
     2401 cggcgtgcgc gccgagggcc tgagcgagga ggagcaggac tgcctgttca tcatcgagac
     2461 cttcgagcgc accctgaccc aggacggcat cgacaactac aacgccgaca tcggcaagct
     2521 gaacaccgcc atcaacctgt acaaccagca gaacaagaag caggagggct tccgcaaggt
     2581 gccccagatg aagtgcctgt acaagcagat cctgagcgac cgcgaggagg ccttcatcga
     2641 cgagttcagc gacgacgagg acctgatcac caacatcgag agcttcgccg agaacatgaa
     2701 cgtgttcctg aacagcgaga tcatcaccga cttcaagaac gccctggtgg agagcgacgg
     2761 cagcctggtg tacatcaaga acgacgtgag caagaccctg ttcagcaaca tcgtgttcgg
     2821 cagctggaac gccatcgacg agaagctgag cgacgagtac gacctggcca acagcaagaa
     2881 gaagaaggac gagaagtact acgagaagcg ccagaaggag ctgaagaaga acaagagcta
     2941 cgacctggag accatcatcg gcctgttcga cgacagcatc gacgtgatcg gcaagtacat
     3001 cgagaagctg gagagcgaca tcaccgccat cgccgaggcc aagaacgact tcgacgagat
     3061 cgtgctgcgc aagcacgaca agaacaagag cctgcgcaag aacaccaacg ccgtggaggc
     3121 catcaagagc tacctggaca ccgtgaagga cttcgagcgc gacatcaagc tgatcaacgg
     3181 cagcggccag gaggtggaga agaacctggt ggtgtacgcc gagcaggaga acatcctggc
     3241 cgagatcaag aacgtggaca gcctgtacaa catgagccgc aactacctga cccagaagcc
     3301 cttcagcacc gagaagttca agctgaactt cgagaacccc accctgctga acggctggga
     3361 ccgcaacaag gagaaggact acctgggcat cctgttcgag aaggagggca tgtactacct
     3421 gggcatcatc aacaacaacc accgcaagat cttcgagaac gagaagctgt gcaccggcaa
     3481 ggagagctgc ttcaacaaga tcgtgtacaa gcagatcagc aacgccgcca agtacctgag
     3541 cagcaagcag atcaaccccc agaacccccc caaggagatc gccgagatcc tgctgaagcg
     3601 caaggccgac agcagcagcc tgagccgcaa ggagaccgag ctgttcatcg actacctgaa
     3661 ggacgacttc ctggtgaact accccatgat catcaacagc gacggcgaga acttcttcaa
     3721 cttccacttc aagcaggcca aggactacgg cagcctgcag gagttcttca aggaggtgga
     3781 gcaccaggcc tacagcctga agacccgccc catcgacgac agctacatct accgcatgat
     3841 cgacgagggc aagctgtacc tgttccagat ccacaacaag gacttcagcc cctacagcaa
     3901 gggcaacctg aacctgcaca ccatctacct gcagatgctg ttcgaccagc gcaacctgaa
     3961 caacgtggtg tacaagctga acggcgaggc cgaggtgttc taccgccccg ccagcatcaa
     4021 cgacgaggag gtgatcatcc acaaggccgg cgaggagatc aagaacaaga acagcaagcg
     4081 cgccgtggac aagcccacca gcaagttcgg ctacgacatc atcaaggacc gccgctacag
     4141 caaggacaag ttcatgctgc acatccccgt gaccatgaac ttcggcgtgg acgagacccg
     4201 ccgcttcaac gacgtggtga acgacgccct gcgcaacgac gagaaggtgc gcgtgatcgg
     4261 catcgaccgc ggcgagcgca acctgctgta cgtggtggtg gtggacaccg acggcaccat
     4321 cctggagcag atcagcctga acagcatcat caacaacgag tacagcatcg agaccgacta
     4381 ccacaagctg ctggacgaga aggagggcga ccgcgaccgc gcccgcaaga actggaccac
     4441 catcgagaac atcaaggagc tgaaggaggg ctacctgagc caggtggtga acgtgatcgc
     4501 caagctggtg ctgaagtaca acgccatcat ctgcctggag gacctgaact tcggcttcaa
     4561 gcgcggccgc cagaaggtgg agaagcaggt gtaccagaag ttcgagaaga tgctgatcga
     4621 caagctgaac tacctggtga tcgacaagag ccgcaagcag gagaagcccg aggagttcgg
     4681 cggcgccctg aacgccctgc agctgaccag caagttcacc agcttcaagg acatgggcaa
     4741 gcagaccggc atcatctact acgtgcccgc ctacctgacc agcaagatcg accccaccac
     4801 cggcttcgcc aacctgttct acgtgaagta cgagaacgtg gagaaggcca aggagttctt
     4861 cagccgcttc gacagcatca gctacaacaa cgagagcggc tacttcgagt tcgccttcga
     4921 ctacaagaag ttcaccgacc gcgcctgcgg cgcccgcagc cagtggaccg tgtgcaccta
     4981 cggcgagcgc atcatcaagt accgcaacgc cgacaagaac aacagcttcg acgacaagac
     5041 catcgtgctg agcgaggagt tcaaggagct gttcagcatc tacggcatca gctacgagga
     5101 cggcgccgag ctgaagaaca agatcatgag cgtggacgag gccgacttct tccgctgcct
     5161 gaccggcctg ctgcagaaga ccctgcagat gcgcaacagc agcaacgacg gcacccgcga
     5221 ctacatcatc agccccatca tgaacgaccg cggcgagttc ttcaacagcg aggcctgcga
     5281 cgccagcaag cccaaggacg ccgacgccaa cggcgccttc aacatcgccc gcaagggcct
     5341 gtgggtgctg gagcagatcc gcaacacccc cagcggcgac aagctgaacc tggccatgag
     5401 caacgccgag tggctggagt acgcccagcg caaccagatc gctagcccaa agaagaagag
     5461 gaaagtctct agataccctt atgatgttcc agattatgcc ggatacccat acgatgtccc
     5521 tgactatgca ggctcctacc cttatgacgt cccagactac gccggatcca ggtccggcgg
     5581 cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggcccaat
     5641 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     5701 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     5761 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     5821 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     5881 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     5941 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     6001 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     6061 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     6121 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     6181 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     6241 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     6301 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaact
     6361 gcagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     6421 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6481 gcattctagt tgtggtttgt ccaaactcat caatgtatct tattaattaa cgtcgacctc
     6541 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6601 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg
     6661 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6721 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6781 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6841 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6901 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6961 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7021 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7081 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7141 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7201 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7261 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7321 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7381 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7441 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7501 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7561 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7621 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7681 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7741 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7801 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7861 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7921 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7981 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8041 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8101 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8161 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8221 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8281 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8341 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8401 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8461 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8521 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8581 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8641 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8701 ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc
     8761 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     8821 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     8881 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     8941 acgggccaga tat
//
