LOCUS       sequence-23589-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Prag G, Watson H, Kim YC, Beach BM, Ghirlando R, Hummer G, 
            Bonifacino JS, Hurley JH
  TITLE     The Vps27/Hse1 complex is a GAT domain-based scaffold for 
            ubiquitin-dependent sorting.
  JOURNAL   Dev Cell. 2007 Jun . 12(6):973-86.
  PUBMED    17543868
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2007 Jun . 12(6):973-86."
COMMENT     Sequence Label: pST39-His/Vps27(345-440) - Hse(275-375)
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..59
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     43..59
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             190..210
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     RBS             545..567
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttccnctgaa ggcctcggat ccgggccctg ntacaagatc ctgtaaaacg acggccagtg
       61 aattccccgg gaagcttcgc cagggttttc ccagtcgagc tcaataattt tgtttaactt
      121 taagaaggag atatacatat gtcgtactac catcaccatc accatcacga ttacgatatc
      181 ccaacgaccg aaaacctgta ttttcagggc gccatgatcc attccgttga tttatctgat
      241 gaggagaaag acagcatata catgtttgct tcacttgtcg aaaaaatgaa atcgaggccc
      301 ttgaacgaaa ttttggaaga ctctaaatta caaaacttgg ctcaaagagt atttgcttcc
      361 aaagcaaggt tgaattatgc tttaaatgat aaagctcaga aatacaatac tttgatagaa
      421 atgaacggca agatttcgga aattatgaac atatacgata gattattgga gcaacagcta
      481 caaagcatca atttgtctca gcaatagggt accagcggat aacaatttca catccggaaa
      541 taattttgtt taactttaag aaggagatat acatatggtt gagccatcga aagaagaaat
      601 tgagaaggaa aaaaataagg aagccatagt attctctcaa aaaactacca tagatcaact
      661 tcataattcg cttaatgcag cttctaaacg ggaaatc
//
