LOCUS       sequence-235924-        3485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 
            promoter for constitutive expression in mosquito cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Fredericks AC, Russell TA, Wallace LE, Davidson AD, Fernandez-Sesma 
            A, Maringer K
  TITLE     Aedes aegypti (Aag2)-derived clonal mosquito cell lines reveal the 
            effects of pre-existing persistent infection with the 
            insect-specific bunyavirus Phasi Charoen-like virus on arbovirus 
            replication.
  JOURNAL   PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0007346. doi: 
            10.1371/journal.pntd.0007346. eCollection 2019 Nov.
  PUBMED    31693659
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2019-11-6"; volume: "13(11):e0007346. doi"; 
            pages: " 10.1371/journal.pntd.0007346. eCollection 2019 Nov"
COMMENT     Sequence Label: pKM50
FEATURES             Location/Qualifiers
     source          1..3485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      110..698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     599..618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1239..2174
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    2205..2326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2216..2235)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2270..2289
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2459..2563
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2564..2632,2633..3424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2782..2801)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
       61 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      121 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      181 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      241 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      301 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      361 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      421 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      481 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      541 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      601 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      661 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      721 tacggttcct ggccttttgc tggccttttg ctcacatggc tcgacagatc aagcttagcg
      781 tgaaccttgc atgcgtgcac agaatttgat tgcatttcat gaccgggtat ccattcggcg
      841 ccggccactg aactgaaatt cagttttgac agctccactg acttgaagct ctttttcacc
      901 gaaacacatc tcggaagaga cacgttggga aagagtaagt ttaatttttg tcgtttttcg
      961 tggattttta gtgataattc gtggtttttc gttgttagca atctaaatca ttgatagatc
     1021 aagttagaaa ttgtgaaata ttgctaaaat acctacatta ttcgtagttt attcgatttg
     1081 ttgtagttga tgcattctaa cctcaatgtg gtttcgtctc cgtccaattc cgatgttatt
     1141 gtccagttcg ttaatttttg gtgttttctc cccgactttt ccacagactg atcgtctgga
     1201 tccaagcttc ccgggcaact ctcgaggtcg acgtgaccat gacttcgaaa gtttatgatc
     1261 cagaacaaag gaaacggatg ataactggtc cgcagtggtg ggccagatgt aaacaaatga
     1321 atgttcttga ttcatttatt aattattatg attcagaaaa acatgcagaa aatgctgtta
     1381 tttttttaca tggtaacgcg gcctcttctt atttatggcg acatgttgtg ccacatattg
     1441 agccagtagc gcggtgtatt ataccagacc ttattggtat gggcaaatca ggcaaatctg
     1501 gtaatggttc ttataggtta cttgatcatt acaaatatct tactgcatgg tttgaacttc
     1561 ttaatttacc aaagaagatc atttttgtcg gccatgattg gggtgcttgt ttggcatttc
     1621 attatagcta tgagcatcaa gataagatca aagcaatagt tcacgctgaa agtgtagtag
     1681 atgtgattga atcatgggat gaatggcctg atattgaaga agatattgcg ttgatcaaat
     1741 ctgaagaagg agaaaaaatg gttttggaga ataacttctt cgtggaaacc atgttgccat
     1801 caaaaatcat gagaaagtta gaaccagaag aatttgcagc atatcttgaa ccattcaaag
     1861 agaaaggtga agttcgtcgt ccaacattat catggcctcg tgaaatcccg ttagtaaaag
     1921 gtggtaaacc tgacgttgta caaattgtta ggaattataa tgcttatcta cgtgcaagtg
     1981 atgatttacc aaaaatgttt attgaatcgg acccaggatt cttttccaat gctattgttg
     2041 aaggtgccaa gaagtttcct aatactgaat ttgtcaaagt aaaaggtctt catttttcgc
     2101 aagaagatgc acctgatgaa atgggaaaat atatcaaatc gttcgttgag cgagttctca
     2161 aaaatgaaca ataattctag agcggccgct tcgagcagac atgataagat acattgatga
     2221 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2281 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2341 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2401 cctctacaaa tgtggtaaaa tcgataagga tccaggtggc acttttcggg gaaatgtgcg
     2461 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2521 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2581 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     2641 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     2701 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     2761 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     2821 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     2881 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     2941 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3001 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3061 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3121 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3181 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3241 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3301 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3361 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3421 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3481 attta
//
