LOCUS       sequence-235932-        4703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  12x Widom 601 nucleosome sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Swygert SG, Senapati S, Bolukbasi MF, Wolfe SA, Lindsay S, Peterson 
            CL
  TITLE     SIR proteins create compact heterochromatin fibers.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Dec 4;115(49):12447-12452. doi: 
            10.1073/pnas.1810647115. Epub 2018 Nov 19.
  PUBMED    30455303
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1810647115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-12-4- 4"; volume: "115"; issue: "49"; pages: 
            "12447-12452"
COMMENT     Sequence Label: pBS_12x-601_MA2
FEATURES             Location/Qualifiers
     source          1..4703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     52..74
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     66..83
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     67..83
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2699..3490
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3583..4171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4072..4091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4325..4342
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4501..4528)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(join(4620..4703,1..3))
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
       61 gacgttgtaa aacgacggcc agtcttaagc gtctccccct gaacctgacc ccggaccaag
      121 tggtggctat tctagataac tgctcttagg cacaggatgt atatatctga cacgtgcctg
      181 gagactaggg agtaatcccc ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt
      241 aagcggtgct agagctgtct acgaccaatt gagcggcctc ggcaccggga ttctccaggt
      301 actggagaat tccagttcca tcagaagcac aggatgtata tatctgacac gtgcctggag
      361 actagggagt aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag
      421 cggtgctaga gctgtctacg accaattgag cggcctcggc accgggattc tccaggtact
      481 ggagaattcg gacgacagtg tgagcacagg atgtatatat ctgacacgtg cctggagact
      541 agggagtaat ccccttggcg gttaaaacgc gggggacagc gcgtacgtgc gtttaagcgg
      601 tgctagagct gtctacgacc aattgagcgg cctcggcacc gggattctcc aggtactgga
      661 gaattcccag ggatccttga gcacaggatg tatatatctg acacgtgcct ggagactagg
      721 gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc
      781 tagagctgtc tacgaccaat tgagcggcct cggcaccggg attctccagg tactggagaa
      841 ttctgtttac accgacggca caggatgtat atatctgaca cgtgcctgga gactagggag
      901 taatcccctt ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag
      961 agctgtctac gaccaattga gcggcctcgg caccgggatt ctccaggttg cgtgggcgga
     1021 atgcgaattc tatcgcacag gatgtatata tctgacacgt gcctggagac tagggagtaa
     1081 tccccttggc ggttaaaacg cggggggtcg accgtacgtg cgtttaagcg gtgctagagc
     1141 tgtctacgac caattgagcg gcctcggcac cgggattctc caggtactgg agaattccgg
     1201 tccgtttacc agcacaggat gtatatatct gacacgtgcc tggagactag ggagtaatcc
     1261 ccttggcggt taaaacgcgg gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt
     1321 ctacgaccaa ttgagcggcc tcggcaccgg gattctccag gtactggaga attcgaaaaa
     1381 gagcgcaagc acaggatgta tatatctgac acgtgcctgg agactaggga gtaatcccct
     1441 tggcggttaa aacgcggggg acagcgcgta cgtgcgttta agcggtgcta gagctgtcta
     1501 cgaccaattg agcggcctcg gcaccgggat tctccaggta ctggagaatt ctacgcatat
     1561 actatgcaca ggatgtatat atctgacacg tgcctggaga ctagggagta atccccttgg
     1621 cggttaaaac gcgggggaca gcgcgtacgt gcgtttaagc ggtgctagag ctgtctacga
     1681 ccaattgagc ggcctcggca ccgggattct ccaggtactg gagaattcgc tcaagcttga
     1741 tcgcacagga tgtatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg
     1801 ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt gctagagctg tctacgacca
     1861 attgagcggc ctcggcaccg ggattctcca ggtactggag aattcagccc acgcagacgg
     1921 cacaggatgt atatatctga cacgtgcctg gagactaggg agtaatcccc ttggcggtta
     1981 aaacgcgggg gacagcgcgt acgtgcgttt aagcggtgct agagctgtct acgaccaatt
     2041 gagcggcctc ggcaccggga ttctccaggt actggagaat tcgcgtgtaa tacactgcac
     2101 agaatgtata tatctgacac gtgcctggag actagggagt aatccccttg gcggttaaaa
     2161 cgcgggggac agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag
     2221 cggcctcggc accgggattc tccaggtaga attcactagt gcggcatgcg gcggcaagca
     2281 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gccgagacgt
     2341 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
     2401 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     2461 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     2521 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     2581 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     2641 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
     2701 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     2761 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     2821 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     2881 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     2941 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     3001 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     3061 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     3121 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     3181 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     3241 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     3301 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     3361 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     3421 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     3481 cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3541 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3601 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3661 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3721 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3781 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3841 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3901 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3961 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4021 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4081 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4141 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4201 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4261 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4321 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     4381 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     4441 cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca
     4501 aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg
     4561 cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga
     4621 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     4681 tccgactgag cctttcgttt tat
//
