LOCUS       sequence-235937-        6827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  24x Widom 601 nucleosome sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Swygert SG, Senapati S, Bolukbasi MF, Wolfe SA, Lindsay S, Peterson 
            CL
  TITLE     SIR proteins create compact heterochromatin fibers.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Dec 4;115(49):12447-12452. doi: 
            10.1073/pnas.1810647115. Epub 2018 Nov 19.
  PUBMED    30455303
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1810647115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-12-4- 4"; volume: "115"; issue: "49"; pages: 
            "12447-12452"
COMMENT     Sequence Label: pBS_24x-601_MA1+MA2
FEATURES             Location/Qualifiers
     source          1..6827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     52..74
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     66..83
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     67..83
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4823..5614
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5707..6295
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6196..6215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6449..6466
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6625..6652)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(join(6744..6827,1..3))
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
       61 gacgttgtaa aacgacggcc agtcttaagc gtctccccct gaacctgacc ccggaccaag
      121 tggtggctat tctagataac tgctcttagg cacaggatgt atatatctga cacgtgcctg
      181 gagactaggg agtaatcccc ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt
      241 aagcggtgct agagctgtct acgaccaatt gagcggcctc ggcaccggga ttctccaggt
      301 actggagaat tccagttcca tcagaagcac aggatgtata tatctgacac gtgcctggag
      361 actagggagt aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag
      421 cggtgctaga gctgtctacg accaattgag cggcctcggc accgggattc tccaggtact
      481 ggagaattcg gacgacagtg tgagcacagg atgtatatat ctgacacgtg cctggagact
      541 agggagtaat ccccttggcg gttaaaacgc gggggacagc gcgtacgtgc gtttaagcgg
      601 tgctagagct gtctacgacc aattgagcgg cctcggcacc gggattctcc aggtactgga
      661 gaattcccag ggatccttga gcacaggatg tatatatctg acacgtgcct ggagactagg
      721 gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc
      781 tagagctgtc tacgaccaat tgagcggcct cggcaccggg attctccagg tactggagaa
      841 ttctgtttac accgacggca caggatgtat atatctgaca cgtgcctgga gactagggag
      901 taatcccctt ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag
      961 agctgtctac gaccaattga gcggcctcgg caccgggatt ctccaggtac tggagaattc
     1021 atgctaacga gatcgcacag gatgtatata tctgacacgt gcctggagac tagggagtaa
     1081 tccccttggc ggttaaaacg cggggggtcg accgtacgtg cgtttaagcg gtgctagagc
     1141 tgtctacgac caattgagcg gcctcggcac cgggattctc caggtactgg agaattccgg
     1201 tccgtttacc agcacaggat gtatatatct gacacgtgcc tggagactag ggagtaatcc
     1261 ccttggcggt taaaacgcgg gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt
     1321 ctacgaccaa ttgagcggcc tcggcaccgg gattctccag gtactggaga attcgaaaaa
     1381 gagcgcaagc acaggatgta tatatctgac acgtgcctgg agactaggga gtaatcccct
     1441 tggcggttaa aacgcggggg acagcgcgta cgtgcgttta agcggtgcta gagctgtcta
     1501 cgaccaattg agcggcctcg gcaccgggat tctccaggta ctggagaatt ctacgcatat
     1561 actatgcaca ggatgtatat atctgacacg tgcctggaga ctagggagta atccccttgg
     1621 cggttaaaac gcgggggaca gcgcgtacgt gcgtttaagc ggtgctagag ctgtctacga
     1681 ccaattgagc ggcctcggca ccgggattct ccaggtactg gagaattcgc tcaagcttga
     1741 tcgcacagga tgtatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg
     1801 ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt gctagagctg tctacgacca
     1861 attgagcggc ctcggcaccg ggattctcca ggtactggag aattcagccc acgcagacgg
     1921 cacaggatgt atatatctga cacgtgcctg gagactaggg agtaatcccc ttggcggtta
     1981 aaacgcgggg gacagcgcgt acgtgcgttt aagcggtgct agagctgtct acgaccaatt
     2041 gagcggcctc ggcaccggga ttctccaggt actggagaat tcgcgtgtaa tacactgcac
     2101 aggatgtata tatctgacac gtgcctggag actagggagt aatccccttg gcggttaaaa
     2161 cgcgggggac agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag
     2221 cggcctcggc accgggattc tccaggtaga attcactaga taactgctct taggcacagg
     2281 atgtatatat ctgacacgtg cctggagact agggagtaat ccccttggcg gttaaaacgc
     2341 gggggacagc gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg
     2401 cctcggcacc gggattctcc aggtactgga gaattcccag ggatccttga gcacaggatg
     2461 tatatatctg acacgtgcct ggagactagg gagtaatccc cttggcggtt aaaacgcggg
     2521 ggacagcgcg tacgtgcgtt taagcggtgc tagagctgtc tacgaccaat tgagcggcct
     2581 cggcaccggg attctccagg tactggagaa ttcggacgac agtgtgagca caggatgtat
     2641 atatctgaca cgtgcctgga gactagggag taatcccctt ggcggttaaa acgcggggga
     2701 cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac gaccaattga gcggcctcgg
     2761 caccgggatt ctccaggtac tggagaattc ccagggatcc ttgagcacag gatgtatata
     2821 tctgacacgt gcctggagac tagggagtaa tccccttggc ggttaaaacg cgggggacag
     2881 cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac caattgagcg gcctcggcac
     2941 cgggattctc caggtactgg agaattctgt ttacaccgac ggcacaggat gtatatatct
     3001 gacacgtgcc tggagactag ggagtaatcc ccttggcggt taaaacgcgg gggacagcgc
     3061 gtacgtgcgt ttaagcggtg ctagagctgt ctacgaccaa ttgagcggcc tcggcaccgg
     3121 gattctccag gttgcgtggg cggaatgcga attctatcgc acaggatgta tatatctgac
     3181 acgtgcctgg agactaggga gtaatcccct tggcggttaa aacgcggggg gtcgaccgta
     3241 cgtgcgttta agcggtgcta gagctgtcta cgaccaattg agcggcctcg gcaccgggat
     3301 tctccaggta ctggagaatt ccggtccgtt taccagcaca ggatgtatat atctgacacg
     3361 tgcctggaga ctagggagta atccccttgg cggttaaaac gcgggggaca gcgcgtacgt
     3421 gcgtttaagc ggtgctagag ctgtctacga ccaattgagc ggcctcggca ccgggattct
     3481 ccaggtactg gagaattcga aaaagagcgc aagcacagga tgtatatatc tgacacgtgc
     3541 ctggagacta gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg
     3601 tttaagcggt gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca
     3661 ggtactggag aattctacgc atatactatg cacaggatgt atatatctga cacgtgcctg
     3721 gagactaggg agtaatcccc ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt
     3781 aagcggtgct agagctgtct acgaccaatt gagcggcctc ggcaccggga ttctccaggt
     3841 actggagaat tcgctcaagc ttgatcgcac aggatgtata tatctgacac gtgcctggag
     3901 actagggagt aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag
     3961 cggtgctaga gctgtctacg accaattgag cggcctcggc accgggattc tccaggtact
     4021 ggagaattca gcccacgcag acggcacagg atgtatatat ctgacacgtg cctggagact
     4081 agggagtaat ccccttggcg gttaaaacgc gggggacagc gcgtacgtgc gtttaagcgg
     4141 tgctagagct gtctacgacc aattgagcgg cctcggcacc gggattctcc aggtactgga
     4201 gaattcgcgt gtaatacact gcacagaatg tatatatctg acacgtgcct ggagactagg
     4261 gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc
     4321 tagagctgtc tacgaccaat tgagcggcct cggcaccggg attctccagg tagaattcac
     4381 tagtgcggca tgcggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     4441 gtgccaggac catggccgag acgtctagac cagccaggac agaaatgcct cgacttcgct
     4501 gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     4561 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     4621 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     4681 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     4741 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     4801 ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact
     4861 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     4921 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     4981 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     5041 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     5101 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     5161 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     5221 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     5281 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     5341 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     5401 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     5461 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     5521 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     5581 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     5641 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5701 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5761 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5821 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5881 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5941 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6001 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6061 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6121 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6181 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6241 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6301 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6361 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6421 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6481 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     6541 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag
     6601 ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt
     6661 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca
     6721 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     6781 cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttat
//
