LOCUS       sequence-235940-       11807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dNmeCas9 with no sgRNA cassette lenti construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, 
            Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller
            CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ
  TITLE     NmeCas9 is an intrinsically high-fidelity genome-editing platform.
  JOURNAL   Genome Biol. 2018 Dec 5;19(1):214. doi: 10.1186/s13059-018-1591-1.
  PUBMED    30518407
REFERENCE   2  (bases 1 to 11807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-12-5"; volume: "19(1):214. doi"; pages: " 
            10.1186/s13059-018-1591-1"
COMMENT     Sequence Label: pLenti_dNmeCas9
FEATURES             Location/Qualifiers
     source          1..11807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        55..266
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      280..289
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             292..312
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3556..3582
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3589..3615
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             3628..3684
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3685..4281
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4178..4198
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4297..4885
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4350..4370)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4768..4779)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4957..5190
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5216..5233)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5222..5446
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5492..5920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5579..5598)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5789..5810
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5929..5949)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5934..6263
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6114..6249
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6176..6195
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6311..6358
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6377..6751
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6881..7002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6918..6937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6972..6991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7051..7067)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7051..7067)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7064..7086)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7075..7091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7099..7105,7106..7123,7124..7129))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7144..7165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7282..7299)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7453..8041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7533..7552)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8212..9003,9004..9072))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8835..8854
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9073..9177)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9252..9271)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9443..9822
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9824..10022
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9977..9997
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10040..10220
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10267..10392
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10885..11118
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11303..11347
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11645..11762
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aattcgctag ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
       61 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg
      121 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      181 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      241 gcaacgggtt tgccgccaga acacaggacc ggttctagag ccaccatggt tccaaaaaag
      301 aaacgcaagg tcgccgcatt caagccaaac agcatcaact acatcctggg actggccatc
      361 ggaatcgcat cagtgggctg ggctatggtc gagatcgacg aggaagagaa ccccatccgg
      421 ctgattgatc tgggcgtgcg agtcttcgaa cgggccgagg tgcctaagac cggtgactcc
      481 ctggctatgg cacggagact ggcccgatct gtcaggcgcc tgacacgacg gagagctcac
      541 cgcctgctgc gaactaggcg cctgctgaag agagaaggag tgctgcaggc cgctaacttt
      601 gacgagaatg gcctgatcaa atccctgcca aatactccat ggcagctgcg agcagccgct
      661 ctggatcgaa agctgacccc actggaatgg agcgccgtgc tgctgcacct gattaagcat
      721 cggggctacc tgtcccagag aaaaaacgaa ggggagacag ctgacaagga actgggagca
      781 ctgctgaaag gagtggccgg caatgcccat gctctgcaga ccggggattt ccggacacct
      841 gcagagctgg ccctgaacaa gtttgaaaaa gagtctggac acatccggaa tcagagaagc
      901 gactattccc atactttcag tagaaaggat ctgcaggccg agctgattct gctgttcgaa
      961 aagcagaaag agtttgggaa cccacacgtg tctggcgggc tgaaagaagg aatcgagaca
     1021 ctgctgatga ctcagagacc cgcactgagt ggcgatgccg tgcagaagat gctggggcat
     1081 tgcaccttcg aacctgcaga gccaaaggca gccaaaaata cctacacagc cgagaggttt
     1141 atctggctga caaagctgaa caatctgcgc attctggaac agggcagcga gaggcctctg
     1201 actgacaccg aacgcgccac cctgatggat gagccatacc ggaagagcaa actgacatat
     1261 gcacaggcca gaaagctgct ggggctggag gacacagcct tctttaaggg gctgagatac
     1321 ggaaaagata acgctgaagc aagtactctg atggagatga aggcttatca cgcaatctca
     1381 cgcgctctgg aaaaagaggg gctgaaggac aagaaatctc ccctgaatct gagtcctgaa
     1441 ctgcaggatg agatcggaac agccttctct ctgtttaaga ctgacgagga tattaccggc
     1501 agactgaaag acagaatcca gcctgaaatt ctggaggctc tgctgaagca catttcattc
     1561 gataaatttg tgcagatcag cctgaaggca ctgcgacgga ttgtcccact gatggaacag
     1621 ggcaaaagat acgacgaagc ctgtgctgag atttacggcg atcattatgg gaagaaaaac
     1681 actgaagaga aaatctatct gccccctatt ccagccgacg agatcaggaa cccagtggtc
     1741 ctgcgagcac tgtcacaggc tcgaaaggtg atcaatggag tggtcagaag gtacggcagc
     1801 cccgctcgaa tccacattga aaccgcacgc gaggtcggaa agtccttcaa agaccggaag
     1861 gaaattgaga agagacagga agagaacagg aaagatcgcg aaaaggctgc agccaaattt
     1921 cgcgagtatt tccctaattt tgtgggcgag cctaagagca aagacatcct gaaactgaga
     1981 ctgtatgagc agcagcacgg aaagtgtctg tacagcggaa aggagattaa cctgggaaga
     2041 ctgaacgaga agggatatgt ggagattgac gctgctctgc cattctcgag aacctgggat
     2101 gatagcttta acaataaggt cctggtgctg ggcagcgaaa accagaataa aggcaaccag
     2161 actccatacg agtatttcaa cgggaaggac aattcccgcg agtggcagga atttaaagcc
     2221 cgagtggaga cctctcgatt cccgcggagt aagaaacagc ggattctgct gcagaagttt
     2281 gacgaggatg gattcaaaga aagaaacctg aatgacacca gatacgtgaa tcgctttctg
     2341 tgccagttcg tcgctgatcg gatgagactg actggcaagg ggaagaaacg ggtgtttgca
     2401 tctaacggcc agatcaccaa tctgctgaga ggattctggg gcctgagaaa agtgagggct
     2461 gaaaacgacc ggcaccatgc cctggatgct gtggtcgtgg cctgtagtac agtggctatg
     2521 cagcagaaga ttactcgctt tgtgcgatat aaagagatga atgccttcga cgggaagacc
     2581 atcgataaag agacaggaga agtgctgcac cagaagacac attttccaca gccctgggag
     2641 ttctttgcac aggaagtgat gatcagagtc tttggcaagc ctgacgggaa accagagttc
     2701 gaggaagccg atactctgga aaagctgagg accctgctgg cagagaaact gagctcccgc
     2761 ccagaggctg tgcacgaata cgtcactccc ctgttcgtgt cccgagcccc taaccggaag
     2821 atgtctggac agggccatat ggaaaccgtg aagagtgcta aacgactgga cgagggcgtg
     2881 agcgtgctga gagtgcccct gacacagctg aagctgaaag atctggagaa gatggtgaat
     2941 agggagcgcg aacctaagct gtatgaggca ctgaaagcca ggctggaagc ccacaaggac
     3001 gatccagcca aagcttttgc agaacccttc tacaagtatg acaaagcagg caaccggacc
     3061 cagcaggtga aggccgtcag agtggagcag gtccagaaaa caggcgtctg ggtcaggaac
     3121 cacaatggga ttgctgataa tgcaaccatg gtccgcgtgg acgtctttga gaagggggat
     3181 aaatactatc tggtgcctat ttacagctgg caggtcgcta agggaatcct gccagacagg
     3241 gcagtcgtgc agggcaaaga cgaggaagat tggcagctga ttgacgattc attcaacttt
     3301 aagttcagcc tgcaccctaa tgatctggtg gaggtcatca caaagaaagc acgcatgttt
     3361 ggctatttcg cctcttgcca tagggggact ggaaacatca atattcgcat ccacgacctg
     3421 gatcataaga tcgggaaaaa cggcatcctg gagggcatcg gggtgaagac agccctgagc
     3481 ttccagaaat accagattga cgagctgggc aaggaaatcc ggccatgtag actgaagaaa
     3541 agaccccccg tacgatatcc ttatgatgtg cctgactatg ccgcagcccc cgcagccaag
     3601 aaaaagaaac tggacggagg atccggcgca acaaacttct ctctgctgaa acaagccgga
     3661 gatgtcgaag agaatcctgg accgaccgag tacaagccca cggtgcgcct cgccacccgc
     3721 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     3781 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     3841 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     3901 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     3961 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     4021 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac
     4081 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     4141 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     4201 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     4261 acccgcaagc ccggtgcctg aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     4321 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4381 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4441 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4501 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4561 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4621 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4681 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     4741 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4801 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4861 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     4921 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     4981 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     5041 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5101 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5161 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     5221 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5281 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5341 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5401 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5461 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5521 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5581 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5641 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5701 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5761 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5821 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5881 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5941 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6001 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6061 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6121 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6181 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     6241 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     6301 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     6361 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     6421 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     6481 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     6541 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     6601 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     6661 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     6721 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     6781 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6841 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     6901 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6961 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7021 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7081 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7141 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7201 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7261 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7321 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7381 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7441 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7501 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7561 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7621 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7681 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7741 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7801 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7861 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7921 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7981 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8041 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8101 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8161 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8221 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8281 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8341 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8401 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8461 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8521 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8581 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8641 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8701 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8761 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8821 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8881 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8941 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9001 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9061 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9121 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9181 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     9241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     9301 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     9361 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     9421 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     9481 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     9541 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     9601 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     9661 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     9721 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     9781 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     9841 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     9901 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     9961 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
    10021 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    10081 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10141 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10201 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    10261 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    10321 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    10381 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    10441 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    10501 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    10561 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    10621 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    10681 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
    10741 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
    10801 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
    10861 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
    10921 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
    10981 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
    11041 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
    11101 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
    11161 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    11221 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    11281 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    11341 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    11401 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    11461 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
    11521 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
    11581 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
    11641 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
    11701 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
    11761 ttcgggttta ttacagggac agcagagatc cagtttggtt aattaag
//
