LOCUS       sequence-235941-        8951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  36x Widom 601 nucleosome sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Swygert SG, Senapati S, Bolukbasi MF, Wolfe SA, Lindsay S, Peterson 
            CL
  TITLE     SIR proteins create compact heterochromatin fibers.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Dec 4;115(49):12447-12452. doi: 
            10.1073/pnas.1810647115. Epub 2018 Nov 19.
  PUBMED    30455303
REFERENCE   2  (bases 1 to 8951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1810647115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-12-4- 4"; volume: "115"; issue: "49"; pages: 
            "12447-12452"
COMMENT     Sequence Label: pBS_36x-601_MA1+MA2+MA3
FEATURES             Location/Qualifiers
     source          1..8951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     52..74
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     66..83
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     67..83
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6947..7738
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7831..8419
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8320..8339
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8573..8590
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8749..8776)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(join(8868..8951,1..3))
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
       61 gacgttgtaa aacgacggcc agtcttaagc gtctccccct gaacctgacc ccggaccaag
      121 tggtggctat tctagataac tgctcttagg cacaggatgt atatatctga cacgtgcctg
      181 gagactaggg agtaatcccc ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt
      241 aagcggtgct agagctgtct acgaccaatt gagcggcctc ggcaccggga ttctccaggt
      301 actggagaat tccagttcca tcagaagcac aggatgtata tatctgacac gtgcctggag
      361 actagggagt aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag
      421 cggtgctaga gctgtctacg accaattgag cggcctcggc accgggattc tccaggtact
      481 ggagaattcg gacgacagtg tgagcacagg atgtatatat ctgacacgtg cctggagact
      541 agggagtaat ccccttggcg gttaaaacgc gggggacagc gcgtacgtgc gtttaagcgg
      601 tgctagagct gtctacgacc aattgagcgg cctcggcacc gggattctcc aggtactgga
      661 gaattcccag ggatccttga gcacaggatg tatatatctg acacgtgcct ggagactagg
      721 gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc
      781 tagagctgtc tacgaccaat tgagcggcct cggcaccggg attctccagg tactggagaa
      841 ttctgtttac accgacggca caggatgtat atatctgaca cgtgcctgga gactagggag
      901 taatcccctt ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag
      961 agctgtctac gaccaattga gcggcctcgg caccgggatt ctccaggtac tggagaattc
     1021 atgctaacga gatcgcacag gatgtatata tctgacacgt gcctggagac tagggagtaa
     1081 tccccttggc ggttaaaacg cggggggtcg accgtacgtg cgtttaagcg gtgctagagc
     1141 tgtctacgac caattgagcg gcctcggcac cgggattctc caggtactgg agaattccgg
     1201 tccgtttacc agcacaggat gtatatatct gacacgtgcc tggagactag ggagtaatcc
     1261 ccttggcggt taaaacgcgg gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt
     1321 ctacgaccaa ttgagcggcc tcggcaccgg gattctccag gtactggaga attcgaaaaa
     1381 gagcgcaagc acaggatgta tatatctgac acgtgcctgg agactaggga gtaatcccct
     1441 tggcggttaa aacgcggggg acagcgcgta cgtgcgttta agcggtgcta gagctgtcta
     1501 cgaccaattg agcggcctcg gcaccgggat tctccaggta ctggagaatt ctacgcatat
     1561 actatgcaca ggatgtatat atctgacacg tgcctggaga ctagggagta atccccttgg
     1621 cggttaaaac gcgggggaca gcgcgtacgt gcgtttaagc ggtgctagag ctgtctacga
     1681 ccaattgagc ggcctcggca ccgggattct ccaggtactg gagaattcgc tcaagcttga
     1741 tcgcacagga tgtatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg
     1801 ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt gctagagctg tctacgacca
     1861 attgagcggc ctcggcaccg ggattctcca ggtactggag aattcagccc acgcagacgg
     1921 cacaggatgt atatatctga cacgtgcctg gagactaggg agtaatcccc ttggcggtta
     1981 aaacgcgggg gacagcgcgt acgtgcgttt aagcggtgct agagctgtct acgaccaatt
     2041 gagcggcctc ggcaccggga ttctccaggt actggagaat tcgcgtgtaa tacactgcac
     2101 aggatgtata tatctgacac gtgcctggag actagggagt aatccccttg gcggttaaaa
     2161 cgcgggggac agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag
     2221 cggcctcggc accgggattc tccaggtaga attcactaga taactgctct taggcacagg
     2281 atgtatatat ctgacacgtg cctggagact agggagtaat ccccttggcg gttaaaacgc
     2341 gggggacagc gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg
     2401 cctcggcacc gggattctcc aggtactgga gaattccagt tccatcagaa gcacaggatg
     2461 tatatatctg acacgtgcct ggagactagg gagtaatccc cttggcggtt aaaacgcggg
     2521 ggacagcgcg tacgtgcgtt taagcggtgc tagagctgtc tacgaccaat tgagcggcct
     2581 cggcaccggg attctccagg tactggagaa ttcggacgac agtgtgagca caggatgtat
     2641 atatctgaca cgtgcctgga gactagggag taatcccctt ggcggttaaa acgcggggga
     2701 cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac gaccaattga gcggcctcgg
     2761 caccgggatt ctccaggtac tggagaattc ccagggatcc ttgagcacag gatgtatata
     2821 tctgacacgt gcctggagac tagggagtaa tccccttggc ggttaaaacg cgggggacag
     2881 cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac caattgagcg gcctcggcac
     2941 cgggattctc caggtactgg agaattctgt ttacaccgac ggcacaggat gtatatatct
     3001 gacacgtgcc tggagactag ggagtaatcc ccttggcggt taaaacgcgg gggacagcgc
     3061 gtacgtgcgt ttaagcggtg ctagagctgt ctacgaccaa ttgagcggcc tcggcaccgg
     3121 gattctccag gttgcgtggg cggaatgcga attctatcgc acaggatgta tatatctgac
     3181 acgtgcctgg agactaggga gtaatcccct tggcggttaa aacgcggggg gtcgaccgta
     3241 cgtgcgttta agcggtgcta gagctgtcta cgaccaattg agcggcctcg gcaccgggat
     3301 tctccaggta ctggagaatt ccggtccgtt taccagcaca ggatgtatat atctgacacg
     3361 tgcctggaga ctagggagta atccccttgg cggttaaaac gcgggggaca gcgcgtacgt
     3421 gcgtttaagc ggtgctagag ctgtctacga ccaattgagc ggcctcggca ccgggattct
     3481 ccaggtactg gagaattcga aaaagagcgc aagcacagga tgtatatatc tgacacgtgc
     3541 ctggagacta gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg
     3601 tttaagcggt gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca
     3661 ggtactggag aattctacgc atatactatg cacaggatgt atatatctga cacgtgcctg
     3721 gagactaggg agtaatcccc ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt
     3781 aagcggtgct agagctgtct acgaccaatt gagcggcctc ggcaccggga ttctccaggt
     3841 actggagaat tcgctcaagc ttgatcgcac aggatgtata tatctgacac gtgcctggag
     3901 actagggagt aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag
     3961 cggtgctaga gctgtctacg accaattgag cggcctcggc accgggattc tccaggtact
     4021 ggagaattca gcccacgcag acggcacagg atgtatatat ctgacacgtg cctggagact
     4081 agggagtaat ccccttggcg gttaaaacgc gggggacagc gcgtacgtgc gtttaagcgg
     4141 tgctagagct gtctacgacc aattgagcgg cctcggcacc gggattctcc aggtactgga
     4201 gaattcgcgt gtaatacact gcacaggatg tatatatctg acacgtgcct ggagactagg
     4261 gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc
     4321 tagagctgtc tacgaccaat tgagcggcct cggcaccggg attctccagg tagaattcac
     4381 tagataactg ctcttaggca caggatgtat atatctgaca cgtgcctgga gactagggag
     4441 taatcccctt ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag
     4501 agctgtctac gaccaattga gcggcctcgg caccgggatt ctccaggtac tggagaattc
     4561 cagttccatc agaagcacag gatgtatata tctgacacgt gcctggagac tagggagtaa
     4621 tccccttggc ggttaaaacg cgggggacag cgcgtacgtg cgtttaagcg gtgctagagc
     4681 tgtctacgac caattgagcg gcctcggcac cgggattctc caggtactgg agaattcgga
     4741 cgacagtgtg agcacaggat gtatatatct gacacgtgcc tggagactag ggagtaatcc
     4801 ccttggcggt taaaacgcgg gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt
     4861 ctacgaccaa ttgagcggcc tcggcaccgg gattctccag gtactggaga attcccaggg
     4921 atccttgagc acaggatgta tatatctgac acgtgcctgg agactaggga gtaatcccct
     4981 tggcggttaa aacgcggggg acagcgcgta cgtgcgttta agcggtgcta gagctgtcta
     5041 cgaccaattg agcggcctcg gcaccgggat tctccaggta cyggagaatt ctgtttacac
     5101 cgacggcaca ggatgtatat atctgacacg tgcctggaga ctagggagta atccccttgg
     5161 cggttaaaac gcgggggaca gcgcgtacgt gcgtttaagc ggtgctagag ctgtctacga
     5221 ccaattgagc ggcctcggca ccgggattct ccaggtactg gagaattcat gctaacgaga
     5281 tcgcacagga tgtatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg
     5341 ttaaaacgcg gggggtcgac cgtacgtgcg tttaagcggt gctagagctg tctacgacca
     5401 attgagcggc ctcggcaccg ggattctcca ggtactggag aattccggtc cgtttaccag
     5461 cacaggatgt atatatctga cacgtgcctg gagactaggg agtaatcccc ttggcggtta
     5521 aaacgcgggg gacagcgcgt acgtgcgttt aagcggtgct agagctgtct acgaccaatt
     5581 gagcggcctc ggcaccggga ttctccaggt tgcgtgggcg gagaaagaat tcgctagcac
     5641 aggatgtata tatctgacac gtgcctggag actagggagt aatccccttg gcggttaaaa
     5701 cgcgggggac agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag
     5761 cggcctcggc accgggattc tccaggtact ggagaattct acgcatatac tatgcacagg
     5821 atgtatatat ctgacacgtg cctggagact agggagtaat ccccttggcg gttaaaacgc
     5881 gggggacagc gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg
     5941 cctcggcacc gggattctcc aggtactgga gaattcgctc aagcttgatc gcacaggatg
     6001 tatatatctg acacgtgcct ggagactagg gagtaatccc cttggcggtt aaaacgcggg
     6061 ggacagcgcg tacgtgcgtt taagcggtgc tagagctgtc tacgaccaat tgagcggcct
     6121 cggcaccggg attctccagg tactggagaa ttcagcccac gcagacggca caggatgtat
     6181 atatctgaca cgtgcctgga gactagggag taatcccctt ggcggttaaa acgcggggga
     6241 cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac gaccaattga gcggcctcgg
     6301 caccgggatt ctccaggtac tggagaattc gcgtgtaata cactgcacag gatgtatata
     6361 tctgacacgt gcctggagac tagggagtaa tccccttggc ggttaaaacg cgggggacag
     6421 cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac caattgagcg gcctcggcac
     6481 cgggattctc caggtagaat tcactagtgc ggcatgcggc ggcaagcaag cgctcgaaac
     6541 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc cgagacgtct agaccagcca
     6601 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
     6661 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     6721 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     6781 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     6841 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     6901 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     6961 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     7021 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     7081 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     7141 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     7201 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     7261 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     7321 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     7381 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     7441 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     7501 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     7561 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     7621 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     7681 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     7741 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     7801 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7861 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7921 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7981 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8041 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8161 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8221 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8281 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8341 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8401 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8461 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8521 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8581 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     8641 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     8701 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc
     8761 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc
     8821 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc
     8881 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc
     8941 tttcgtttta t
//
