LOCUS       sequence-235942-        2143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part plasmid for J23101 (strength 
            1791x Anderson promoter; see 
            http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2143)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P1m
FEATURES             Location/Qualifiers
     source          1..2143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(84..875,876..944))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     707..726
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(945..1049)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1117..1135
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1139..1182)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1185..1206
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        1225..1259
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     misc_feature    1271..1291
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1297..1314)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1468..2056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1548..1567)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
       61 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
      121 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
      181 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg
      241 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      301 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      361 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      421 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
      481 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
      541 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
      601 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
      661 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
      721 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      781 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      841 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      901 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
      961 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1021 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     1081 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     1141 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt
     1201 ctagagacta gtgggtctca ggagtttaca gctagctcag tcctaggtat tatgctagct
     1261 actagagacc tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc
     1321 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1381 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1441 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     1501 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1561 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1621 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1681 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1741 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1801 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1861 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1921 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1981 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2041 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2101 cgaaaactca cgttaaggga ttttggtcat gagattatca aaa
//
