LOCUS       sequence-235946-        9507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible mouse Tcf3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Wang Q, Zou Y, Nowotschin S, Kim SY, Li QV, Soh CL, Su J, Zhang C, 
            Shu W, Xi Q, Huangfu D, Hadjantonakis AK, Massague J
  TITLE     The p53 Family Coordinates Wnt and Nodal Inputs in Mesendodermal 
            Differentiation of Embryonic Stem Cells.
  JOURNAL   Cell Stem Cell. 2017 Jan 5;20(1):70-86. doi: 
            10.1016/j.stem.2016.10.002. Epub 2016 Nov 23.
  PUBMED    27889317
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.10.002"; journalName: "Cell Stem Cell"; date: 
            "2017-01-5- 5"; volume: "20"; issue: "1"; pages: "70-86"
COMMENT     Sequence Label: pLVXT-mTcf3
FEATURES             Location/Qualifiers
     source          1..9507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1820..2319
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1855..1878)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2252..2271
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2340..2939
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2340..2359)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2836..2856
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2956..3544
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3009..3029)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3427..3438)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3751..4384
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4512..4528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4512..4528)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4525..4547)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4536..4552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4560..4566,4567..4584,4585..4590))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4605..4626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4743..4760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4914..5502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4994..5013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5673..6464,6465..6533))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6296..6315
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6534..6638)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6686..6820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6723..6742)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6777..6796
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6988..7621
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7668..7793
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8290..8523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8708..8752
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9015..9130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        9193..9507
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9201..9219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9237..9255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9272..9290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9308..9326
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9344..9362
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9379..9397
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9415..9433
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     9480..9504
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tggagaagga tccgcggccg cgccggctct agatcgcgaa cgcgtgccac catgccccag
       61 ctcggtggtg gccgcggtgg cggcgccggc gggggaggcg gtggctccgg agccggggca
      121 accagtggag gggacgacct cggagcgaac gacgagctga tccccttcca ggacgagggg
      181 ggcgaagagc aagagccgag cagcgacagc gcttcggcgc agagggatct ggatgaggtc
      241 aagtcgtccc tggtcaacga atcggagaat cagagcagta gctcggactc cgaggcggag
      301 aggcgcccgc agcccgcccg ggacgctttc cagaagccgc gcgactattt cgccgaagtg
      361 agaaggcccc aggatggcgc tttttttaag ggacctgcgt atcctgggta ccccttcctg
      421 atgattccag acctgagcag cccgtacctc tccaacggac ccctgtctcc tggtggagcg
      481 cgcacctacc tacagatgaa atggcccctc ctcgatgtcc cctccagtgc tacagtcaag
      541 gacacaaggt cgccatctcc agcacacttg tccaacaaag ttcctgttgt tcagcaccct
      601 catcacatgc acccgctgac acctctcatc acctacagca acgaccactt ctcccctgcg
      661 tcccctccca cgcatctgtc cccagagatc gacccaaaga caggaatccc ccggccccct
      721 catccatctg agctgtcacc gtattaccca ctgtctccag gagctgtcgg acaaatcccc
      781 catcccctcg gctggctcgt cccacagcaa gggcagccca tgtactctct ccctccgggt
      841 ggtttccggc atccttaccc tgccctcgcc atgaacgctt caatgtccag cctggtctca
      901 agccggttcc cacacatggt ggctcctgcc catcctggtc tgcccacctc aggaatcccc
      961 caccctgcca tcgtctcccc cattgtgaag caggagccag cagcccccag cctgagccct
     1021 gcagtgagtg cgaaatcccc agttacggtg aagaaggaag aggagaagaa acctcacgtg
     1081 aaaaagcccc tgaatgcctt catgttgtat atgaaggaga tgagggccaa ggtggtggcc
     1141 gagtgtaccc tgaaggaaag tgcagccatt aaccaaatcc tgggaagaaa gtggcacaac
     1201 ctgtcaagag aagaacaggc caaatactat gagcttgccc ggaaagaacg gcagcttcac
     1261 gcgcagctct acccaacctg gtcagcccgg gacaactatg ggaagaagaa gaagaggaag
     1321 agagaaaagc agctgtcaca gacacagtct cagcagcaaa tccaagaggc agagggtgct
     1381 ctggcctcta agagcaagaa gccatgcatt cagtacctgc cccctgagaa gccttgtgat
     1441 agccctgcgt cttcccatgg cagcacgctg gactcccctg cgacgccctc cgcagccttg
     1501 gcctcaccag ctgcccctgc ggctacccac tccgagcaag cgcagcccct gtcgctcacc
     1561 accaaaccgg aggcccgggc ccagctggct ctccactccg ctgccttcct gtcggctaag
     1621 gcggccgcca gcaactccag ccagatgggc agccagcccc cactcctatc caggcccttg
     1681 cccttgggct ctatgcccgc cgctctgctg acctctcccc ctactttccc agctacgctc
     1741 catgcccacc aggccctccc cgtgctacag gcccagcctc tttccttggt caccaagtct
     1801 gcccactaag aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     1861 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     1921 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     1981 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     2041 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     2101 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     2161 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     2221 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     2281 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     2341 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     2401 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     2461 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     2521 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     2581 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     2641 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     2701 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     2761 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     2821 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     2881 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     2941 gggcgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3001 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3061 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3121 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3181 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3241 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3301 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     3361 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3421 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3481 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3541 ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca
     3601 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     3661 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     3721 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
     3781 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     3841 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     3901 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     3961 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     4021 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     4081 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     4141 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     4201 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     4261 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     4321 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     4381 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     4441 tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg
     4501 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4561 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4621 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4681 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4741 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4801 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5101 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5341 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5461 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5581 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5641 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5701 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5761 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5821 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5881 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5941 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6001 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6061 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6121 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6181 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6241 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6301 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6361 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6421 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6481 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6541 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6601 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6661 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
     6721 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6781 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     6841 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
     6901 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     6961 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
     7021 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     7081 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     7141 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     7201 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     7261 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     7321 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     7381 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     7441 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7501 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7561 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7621 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     7681 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     7741 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     7801 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     7861 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     7921 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     7981 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     8041 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     8101 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     8161 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     8221 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     8281 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     8341 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     8401 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     8461 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     8521 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     8581 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     8641 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     8701 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     8761 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     8821 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     8881 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     8941 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     9001 tcgacggtat cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     9061 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     9121 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttatcga tgaggccctt
     9181 tcgtcttcac tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc
     9241 tatcagtgat agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     9301 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
     9361 gaacgtatgt cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     9421 tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga
     9481 gctcgtttag tgaaccgtca gatcgcc
//
