LOCUS       sequence-235947-        2126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for T7 promoter (strong, T7 
            polymerase-dependent constitutive promoter; use with C95m). Please 
            see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2126)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P18m
FEATURES             Location/Qualifiers
     source          1..2126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..184
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(185..253,254..1045)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(403..422)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1216..1804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1705..1724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1958..1975
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1981..2001
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    2049..2070
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2073..2116
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(join(2120..2126,1..12))
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
       61 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      121 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      181 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      241 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      301 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      361 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      421 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      481 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      541 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      601 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      661 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      721 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      781 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      841 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      901 gtcgcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      961 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1021 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1081 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1141 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1201 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1261 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1321 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1381 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1441 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1501 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1561 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1621 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1681 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1741 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctgtg
     1801 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1861 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1921 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1981 ctgcagcggc cgctactagt aggtctctag tactatagtg agtcgtatta ctcctgagac
     2041 ccactagtct ctagaagcgg ccgcgaattc cagaaatcat ccttagcgaa agctaaggat
     2101 tttttttatc tgaaattctg cctcgt
//
