LOCUS       sequence-235948-        2199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pLbda-CasRQi (strong lambda 
            promoter with downstream dCas binding site for Qi-lab gRNA (UC16m); 
            gRNA sequence taken from DOI: 10.1016/j.cell.2013.02.022)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2199)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P35m
FEATURES             Location/Qualifiers
     source          1..2199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    75..95
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(101..118)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(272..860)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(352..371)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1031..1822,1823..1891))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1654..1673
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1892..1996)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2064..2082
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2086..2129)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2132..2153
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ctctggcggt gataatgatg gagtccagac accggctttc cccagaccgc taaactgaaa
       61 gtttactaga gacctactag tagcggccgc tgcaggcttc ctcgctcact gactcgctgc
      121 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      181 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      241 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc
      301 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      361 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      421 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      481 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      541 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      601 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      661 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
      721 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      781 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
      841 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
      901 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
      961 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1021 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1081 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1141 catctggccc cagtgctgca atgataccgc gcgacccacg ctcaccggct ccagatttat
     1201 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1261 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1321 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1381 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1441 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     1501 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     1561 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     1621 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     1681 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1741 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1801 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     1861 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     1921 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     1981 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     2041 ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa
     2101 aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta gagactagtg
     2161 ggtctcagga gctaacaccg tgcgtgttga ctattttac
//
