LOCUS       sequence-235961-        2349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pCin* (Adam Meyer's improved 
            pCin promoter from https://doi.org/10.1101/285866, selected for 
            dynamic range and less cross-sensitivity)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2349)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P57m
FEATURES             Location/Qualifiers
     source          1..2349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    150..170
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(176..193)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(347..935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(427..446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1106..1897,1898..1966))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1729..1748
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1967..2071)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2139..2157
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2161..2204)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2207..2228
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gcgcatgtcg ttatcgcaaa accgctgcac acttttgcgc gacatgctct gatccccctc
       61 atctgggggg gcctatctga gggaatttcc gatccggctc gcctgaacca ttctgctttc
      121 cacgaacttg aaaacgctta ctagagacct actagtagcg gccgctgcag gcttcctcgc
      181 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      241 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
      361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      541 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      781 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1021 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1081 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1141 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1201 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgcgac ccacgctcac
     1261 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1321 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1381 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     1441 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     1501 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     1561 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     1621 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     1681 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     1741 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     1801 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     1861 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     1921 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     1981 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2041 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     2101 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     2161 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
     2221 ttctagagac tagtgggtct caggagaacg atcgttggct gccctttgtg cgtccaaacg
     2281 gacgcacggc gctctaaagc gggtcgcgat ctttcagatt cgctcctcgc gctttcagtc
     2341 tttgttttg
//
