LOCUS       sequence-235964-       10757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning 
            homology arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10757)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 10757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1592
FEATURES             Location/Qualifiers
     source          1..10757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..306
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1242..1262
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1280..1313)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1323..1352
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3169..3492)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5410..5434
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5437..6462
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5437..6462
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6463..6487)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6840..6873)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7015..7146
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             7174..7197
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7201..7224
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7228..7251
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(7299..7604)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7870..7887)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7870..7886)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7879..7901)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8095..8114)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8214..8236
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8274..8292)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8360..8464
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8465..8533,8534..9325)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8683..8702)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9496..10084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9985..10004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10238..10255
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10372..10393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10408..10413,10414..10431,10432..10438)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10446..10462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10451..10473
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10470..10486
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10470..10486
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
       61 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      121 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      181 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      241 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      301 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
      361 gagcctcagg agcatcgatg agtaaaggag aagaattgtt cactggagtt gtcccaatcc
      421 tcgtcgagct cgacggagac gtcaacggac acaagttctc cgtctccgga gagggagagg
      481 gagacgccac ctacggaaag ctcaccctca agttcatctg caccaccgga aagctcccag
      541 tcccatggcc aaccctcgtc accaccttct gctacggagt ccaatgcttc tcccgttacc
      601 cagaccacat gaagcgtcac gacttcttca agtccgccat gccagaggga tacgtccaag
      661 agcgtaccat cttcttcaag gtaagtttaa acatatatat actaactact gattatttaa
      721 attttcagga cgacggaaac tacaagaccc gtgccgaggt caagttcgag ggagacaccc
      781 tcgtcaaccg tatcgagctc aaggtaagtt taaacagttc ggtactaact aaccatacat
      841 atttaaattt tcagggaatc gacttcaagg aggacggaaa catcctcgga cacaagctcg
      901 agtacaacta caactcccac aacgtctaca tcatggccga caagcaaaag aacggaatca
      961 aggtcaactt caaggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1021 ttcagatccg tcacaacatc gaggacggat ccgtccaact cgccgaccac taccaacaaa
     1081 acaccccaat cggagacgga ccagtcctcc tcccagacaa ccactacctc tccacccaat
     1141 ccgccctctc caaggaccca aacgagaagc gtgaccacat ggtcctcctc gagttcgtca
     1201 ccgccgccgg aatcacccac ggaatggacg agctctacaa ggagaatctg tactttcaat
     1261 ccggaaaggt aagtttaaaa taacttcgta tagcatacat tatacgaagt tattttcagg
     1321 gagagcaaaa gcttatctcc gaggaggacc tctaaggatg atcgacgcca acgtcgttga
     1381 attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt tattctcttt
     1441 gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca tttttttttc
     1501 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat
     1561 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca
     1621 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa
     1681 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aactagctgc
     1741 cctcgattgt ctatcttcca ttctcaccaa gtttcaacaa ctttattcag tcattcttct
     1801 ctggttcatt tcccaaccaa tcttcccgaa taaagcccat ctgtcctttc ggccacattc
     1861 ctcacctaca tccgtactcc ttatctcccg ttcttatcca gtcttttatc gtctctgcgt
     1921 ctcgcacact gtctcgtcgg taatgtaaaa aaaaaacaaa aaaaacttga aaacttgcgg
     1981 aaagatttcc taataaaagg agacgaatcc ggtaggaaat cgacaagtct ctgcttctcg
     2041 aagactaggt gagttacatt tctttggccg aattattttg aaatcctaaa tttgataatt
     2101 tcagagatgt ctgtaaaact tgcgtgttat gtgacggctt cggtcactgt cgccactctt
     2161 atggtatgct tcatgaccat gtcaaccatc tactcggagg ttgatggatt cagagagaag
     2221 cttgataccg agatgaatgt gttcagagtg agttaaatta tattttgaat tttaaataat
     2281 ttttaatttt ctagcaatct accaatggat tgtggaagga catagttgtc atcggaagat
     2341 ctagcaagcg tgtccgttgt caatatgaag agaccaacgc taccccaact ccacatgctg
     2401 atggatcccc atctgctcca ccaggtcaac caccagcagt tccaccagtc ttcaaccagc
     2461 caaagactcc aaatggagcc aatggaaatg gaccaacctg caactgcaat gctgataaca
     2521 agtgcccagc tggaccatcc ggaccaaagg gagttccagg agttccagga ctcgacggag
     2581 ttccaggact tgacggtgtt ccaggagttg gagctgatga tatcgctcca caacgcgagt
     2641 ctgtcggatg cttcacttgc ccacaaggac cagttggacc accaggagct cttggaagac
     2701 caggaccacg tggacttcca ggaccaagag gacaaaatgg aaacccagga agagatggac
     2761 aaccaggaca tccaggagag caaggatcat ccggtcaaat cggaaagatc ggagagccag
     2821 gaccaccagg agagaaggga cgcgacgccg agcatccaat cggaagacca ggaccaaagg
     2881 gaccaagagg agatcaagga ccaacaggac cagctggaca gaacggtctt cacggaccac
     2941 caggagagcc aggaaccgtt ggaccagaag gaccatctgg aaagcaagga cgtcaaggac
     3001 cagacggaac ccagggagag actggaccag acggaagacc aggaaaggat gccgagtact
     3061 gccagtgccc agacaagtct ccaccatcag aggctgtcaa cgccaaccgt ggatacagaa
     3121 atatctaaat tgttggtgtt ttctaataaa atatttgaga ttcagtgaga cttttttctt
     3181 ggcggcacaa taaagtttcc tctttttgtt gggtcactca acgaacacaa tacaaatatt
     3241 tgagcgctcg catttttctc aacctttcgg gtttaaagca cttcaaaata gaattgaaga
     3301 gagattgtga aagtacatca gacagaatca cattttgata gcattcactt cactcagatg
     3361 caaacttttc agtgactata aaaagtcata aaataataac attaaaaaag caaataaatt
     3421 agcgagagaa ttttttgaca aaaagaaaga agagtgatag agaagaaggg aatgcttgaa
     3481 aggattttgc atttatctta atcaccgtct tcgagaagac gaaccatggc gccagtttca
     3541 gaatccaagt ttctaatata gttcatcaca atgttgacgt tggtccaacc tccggcttgc
     3601 ataatctctg ggatggaaac tccagcgcga gccatgtcac gggcagctcc gacacgggcg
     3661 gaatgtccag accaagccaa gtaacgttgt ccggaatcat ccttggctcc gtagatgaga
     3721 cggtgggtag cttcgaagat tcctgaaaat ttattttcag cttattctta gctaatatgt
     3781 tgtttaaact taccttcaag agctcgagtc gagagttgtg atgttgcact cggggcagca
     3841 actccgttct tgcgaacacg acagaacagg tagttgtttg gatcatcggc gactccggaa
     3901 accgaaatcc agcgctcaac gagcttggta actccaaggg agagcgcttt ttcgactccg
     3961 gcagtcgaga ccagcgtttt tgtacgtccg atgtggatga gcattcttcc tccgtctgtg
     4021 cgactaatat ccttgacgcg aatgcgggca atttcggcga tacggagaag tgtgttgtaa
     4081 gcaattccga ggaaggcgag gtttcggatg tcctggcaac gatcggaatt ttccataaga
     4141 gaacggacct gatcgaagtc ggttctgaaa atttcgtcaa gtcttaggat tgaatacatt
     4201 aatatttaaa cttacctttc gaaggcgagg gcttgcttgg ctctctctcc agcatcaaca
     4261 ttttccttgc ggatacgtct cattacgaga gatacggcat tagaatctga tgggcgtgga
     4321 agtccagatc ggcgatggag catattgagc tgtccgagat gttgttggat cgtcttcacc
     4381 gccaatccac gagcttggag gtagagcaag tagtcacgaa cgtcttctgg ctcggctggg
     4441 aaccacttgc ggttgttaag cttgcaccag gcagcccaag aacggcacac tgagagaagc
     4501 atcttccagg tgtgctcgga gaaggcttgt ctgtcacgga acatatccat gaggttcttg
     4561 cgaacttcat cacttgtcgc gtcaactgga agtgctggaa gattttgatg aacagtgaga
     4621 agatttgaca tattttcgaa gttttttaga tgcactagaa caaagcgtgt tggcttcctc
     4681 tgagcccgct ttccttatat acccgcattc tgcagcctta cagaatgttc tagaaggtcc
     4741 tagatgcatt cgtttgaaaa tactcccggt gggtgcaaag agacgcagac ggaaaatgta
     4801 tctgggtctc tttattgtgt acactacttt tccatgtacc gaatgtgagt cgccctcctt
     4861 ttgcaacaag cagctcgaat gttctagaaa aaggtggaaa atagtataaa taccgttgaa
     4921 aataaatacc gaacaacatt tgctctaatt gtgaaattag aaatcttcaa actataatca
     4981 tgtcacttta ccactatttc cgtccagctc aacgttccgt ttttggtgat catttttgct
     5041 ttcgtcgtaa atctacacac gcgtctcttc cgtgcgagag tccaagccag cagccaaatt
     5101 cgttgactga gtattcaacg tttatacgtt gtcggcaacg agaaatagga aaatgcatcg
     5161 ggaaatgttc ttttttcgat tttttccaag gttttgacaa attttaccac gaattttgct
     5221 atgttttcaa ttaaaaaata tgttattcaa ctgtttctat gaggaaaata aggctttgca
     5281 tgtaattttc ttattcagca taatttttaa ttaatttgaa ttttctgtcc taacgtttat
     5341 tttgttttct tggttatgac tgatctgaaa ttaatttttg aattttaagg taatatgtca
     5401 ggcggtgccg caagtttgta caaaaaagca ggctccatga aaaagcctga actcaccgcg
     5461 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     5521 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     5581 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     5641 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     5701 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     5761 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     5821 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     5881 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     5941 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     6001 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     6061 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     6121 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     6181 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     6241 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     6301 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     6361 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     6421 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agacccagct ttcttgtaca
     6481 aagtgggtcc aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc
     6541 ctatttttgt tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag
     6601 tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc
     6661 gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg
     6721 ttctgtgtac acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa
     6781 aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttaa
     6841 taacttcgta tagcatacat tatacgaagt tattttcagg gagccggatc tgattacgga
     6901 tccggaggtg gcgggatggc ttcttctctt cgtcagatcc tcgactctca gaagatggag
     6961 tggcgttcta acgctggagg atctggcgga ggaggcagtg gtagtggcag tatgcctaaa
     7021 gatccagcca aacctccggc caaggcacaa gttgtgggat ggccaccggt gagatcatac
     7081 cggaagaacg tgatggtttc ctgccaaaaa tcaagcggtg gcccggaggc ggcggcgttc
     7141 gtgaagggtg gtggcggttc tggctcagga tctgattata aagacgatga cgataagcgt
     7201 gactacaagg acgacgacga caagcgtgat tacaaggatg acgatgacaa gagactagtt
     7261 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg
     7321 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
     7381 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata
     7441 tgcaccaccg ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg
     7501 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga
     7561 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt
     7621 ctcgtcggca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg
     7681 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct
     7741 taccgaaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca
     7801 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtaacta
     7861 gtgccggcga ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7921 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     7981 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     8041 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     8101 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     8161 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     8221 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     8281 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     8341 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8401 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8461 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8521 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8581 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8641 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8701 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8761 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8821 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8881 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8941 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9001 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9061 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9121 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9181 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9241 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9301 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9361 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9421 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9481 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9541 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9601 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9661 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9721 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9781 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9841 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9901 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9961 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10021 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10081 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10141 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    10201 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    10261 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    10321 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    10381 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    10441 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatt tatcgatttc
    10501 ctaggaaccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
    10561 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggttcggt
    10621 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
    10681 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgccgacga
    10741 gaacaggggc tggtgaa
//
