LOCUS       sequence-235965-        6954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for generating kank1a riboprobes for WISH in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Hensley MR, Cui Z, Chua RF, Simpson S, Shammas NL, Yang JY, Leung 
            YF, Zhang G
  TITLE     Evolutionary and developmental analysis reveals KANK genes were 
            co-opted for vertebrate vascular development.
  JOURNAL   Sci Rep. 2016 Jun 13;6:27816. doi: 10.1038/srep27816.
  PUBMED    27292017
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-06-13"; volume: "6:27816. doi"; pages: " 
            10.1038/srep27816"
COMMENT     Sequence Label: kank1a_pJet1.2
FEATURES             Location/Qualifiers
     source          1..6954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    91..112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(127..132,133..150,151..157)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(4619..4638)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4620..4638)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4944..4962)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5030..5134
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5135..5203,5204..5995)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5353..5372)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6166..6754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6655..6674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6908..6925
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcaatt ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      121 ccaggcttta cactttatgc ttccggctcg tataatgtgt ggaattatga gcggataata
      181 atttcacaca ggaggtttaa actttaaaca tgtcaaaaga gacgtctttt gttaagaatg
      241 ctgaggaact tgcaaagcaa aaaatggatg ctattaaccc tgaactttct tcaaaattta
      301 aatttttaat aaaattcctg tctcagtttc ctgaagcttg ctctaaacct cgttcaaaaa
      361 aaatgcagaa taaagttggt caagaggaac atattgaata tttagctcgt agttttcatg
      421 agagtcgatt gccaagaaaa cccacgccac ctacaacggt tcctgatgag gtggttagca
      481 tagttcttaa tataagtttt aatatacagc ctgaaaatct tgagagaata aaagaagaac
      541 atcgattttc catggcagct gagaatattg taggagatct tctagaaaga tatggcccaa
      601 accatgcaca ttaacggcaa cgcctcagaa aaagatgaag atgagaagga cccctactgc
      661 ttggaaaccc catatgggta tcagctagac ctggacttcc ttaaatatgt agatgacatt
      721 gagaggggca acaccatcaa gaagctgaac atccagagga agccaaaagt ggtcaggact
      781 gttccgccgc ctcggagcag ttgcagtggc cacactgagt ggacatcaac agactccttg
      841 tcctcttcca acagtgatga atgcaaacag tcaccagtct atttcacgtc tcagaaccag
      901 gtagcccctc ctggtcatac tgcccaagtc catgaagttc cccaaccctt tgtgaacgtc
      961 cctgagacca agcagctgct tcctccacct tcgcctcgtc ttcttcgtca taacccgcag
     1021 gtggagaaaa cactgatgga aactcgaagg agactggagc aggagcggct cctgatgcag
     1081 ccaccagtca gtgagccacc tcgtaggcga ttagccagtt ttggaggaat ggggtcttcc
     1141 agttccctgt cctcttacag tgggtcctat ggaccaagcc agatttctcc cagctcccat
     1201 tctttccaac ataatggtca tctaggcaat ggggaataca acccctactt cacatcctct
     1261 atgggcagtt caatccgcca cagtcccctg agctcaggca tgaccacacc agtgaccaat
     1321 gttagcccct tgcacctcca gcacattcga gaacagatgg tagtagcgct aaagagacta
     1381 aaagagttgg aggagcaggt aaagacaatc ccaatccttc aagtcaagat ctcagtcttg
     1441 caagaagaga aaagacaact agtatcccag aagaataact ctaaggtagt ggggcaagga
     1501 tcaggtggat gtttcaggaa gcgctccttt agcgttggca gtgctgatca atatgagccc
     1561 ctgtcccaga tcaagacagg ttctgagctt cacattgagg agattgataa cactgagcag
     1621 agcactcaaa ggctacagga gttcaggcaa ttaacggctg aagtggaagc cctagagcgg
     1681 aacatacagg ataatggtga ggaaattaag cacagcttac accaaacaga gcaaagatca
     1741 aaccaaaatg tccagctgtg gagccgtgaa agcaggtcca ttggtgtcgg agctgatgag
     1801 aacatgaacg atgtggtggt ttacaggagg tcacctggca aaaacaaaga tgtggctgtt
     1861 ggaactgaac aggaggtgag gagcacagga gttggtgtga ctgaagccat gctaggcctg
     1921 agcagtgagg cagaaactga aattgagatg cagcaccaga ccatcgaagc cctcaaagag
     1981 aagatttaca gattggaggt tcaacttaag gaaacgaccc accaaatgga aatgggaaag
     2041 ctaaagcttc aactgcaagt tgctggatct aggaaaaagg cagacaaagg cttgatggca
     2101 aggccagaaa tgtacagcac gtcagtggag gcaagagtgt ccacacatag ccaaggtgtg
     2161 ggcaaccatg ttgagcttag tgatgcaagc acaaaccatg tccatcaaat gaattcagtg
     2221 ggcattactt gcagaccgga tgcaaggcat gttttagtgg gtccagagtt gccaatggat
     2281 cgatggattg tacaagaacg tactgaggtt caagatcagt gtgtcggcag gcatgttgtg
     2341 atgtgcagcc agagtgtggg agtggaatta agtgtatgtg aaatgggaat caacacggag
     2401 ttaacagctg aaggtttagg actttgcaaa agagaggctg aacaagccaa ggagttcagg
     2461 tctataggat gtggagattg ttcagtggat gtaatggtta gacccattaa ggagatggta
     2521 actcggagta caggaacaga tgaagttcac agaaatgact ttggagtcat ggtcttaccc
     2581 atatgtgcgt cacagtacac aaatatggaa attgagacaa tagacaagtt aaccaataca
     2641 gacaagttag ttcttactga tacctccacc aatacacaat catgctcaaa agacaaacaa
     2701 gtcagcaccg aaccgttaga gacacgtaca atcgctgttg gtgatggtct tgtaaaagat
     2761 gcgccagcca cattaaaaac ccgttcagtt tcagtaggta ctacttcatc agacgatgct
     2821 tttcttgaca agttgccatc ttgcaaaacc aaagagactg gaattggcca cacaagcata
     2881 cacgaaaact tcttagtcgg tcagaagaca aggaacattg cctgtggccc ctctcagtct
     2941 cctaccccca cccaagttaa gagtgatttt gaggaatctg tcttaacaga agggcccaac
     3001 cagtcacagg ctggtgcagg agttggactt gatcactaca tagaacgagt gcaaaagctc
     3061 ttgcaggaac agcagatgtt gctggctcag aactacagtg agctggcaga tgcattcggc
     3121 ccacctcaac aatcccagtt cagttccatc aacagtgaac tagtcactac tctatcatcc
     3181 atcaattcag tgtggaagta tggaagtctt gaagaactct gtaacttgga tcttctaagg
     3241 ccgcaggaga actcaaatca caccgtgaaa gagacacaga tggtcagtgc agtccattcc
     3301 cactcgagca gtttgaggtc agaggtcacg cggatagaga gtgtgtgcac agacgacaaa
     3361 catgaacaga gtaccgctgc gcagaaaagt cgcatggacc agcaaatgtc cacagcattg
     3421 catggcagtc acagcaccct gaagtccatc atgaagaaag atggccatca gggacacaat
     3481 ggcacaaaaa agaacttgca gtttgttgga gtaaatggag gctatgagac gacgtcgagc
     3541 gatgactcca gctcagagga gagcagctct tctgggtcag aggatgaagg ggaagacaaa
     3601 aacaagacag taaatgtgag agaaaggagg aaagaagaat tcaggaataa tgaagccgtg
     3661 gatgaagaca gagacactga agatttaggc caggataaaa ggcagaggta tgagctgagt
     3721 gagaagatgc tttcagcatg caacatcctg aaatcccacc tgaacgaccc caaggcttta
     3781 tctagtaaag atgtgaggcc gtgtgtgaac accgtgcagc atgagtggtt ccgagtgtcc
     3841 agtcagaaga cggctgtggc ggagatggtg gaggactatc tgaatgcgtt cagaagcatc
     3901 tctcctgccc tgctgcagca cattgtaaac atggccgatg gcaacggcaa caccgccctc
     3961 cattacagcg tgtcccattc caacttccac attgtcaaga agctactgga agctgatgtc
     4021 tgcaatgtca atcagcagaa taaagcaggc tatacgccca tcatgcttgc agcgctcgcc
     4081 gcagtagagg ctccgaagga catgagggtg gtcgatgagc tgtttagtaa aggagatgtc
     4141 aatgcgaagg ccagccaggc tggtcagaca gggctgatgc tggctgtcag tcatggcaga
     4201 atggacatgg tgaaagctct gctagcctgt ggtgcggatg tcaacatcca ggacgacgag
     4261 ggctcgaccg cgctcatgtg tgccagtgaa cacggccatg tggagatagt gaagctgctg
     4321 ctggctcagc caggctgtga tgccactctc agtgataatg acgaaagcaa cgcattgtct
     4381 attgctctgg aagcaggaca taaggacatc gcagtcttgc tgtacgctca tatgaacttc
     4441 tccaaagctc agtcaccagg aactcctcga ctcggaagga agacttcacc cagccctaca
     4501 agaaaaggtc ctgtttgatt tagacagcgt ctacagcacc catctgagct gctattcctt
     4561 caggttctgt catcttgctg aaaaactcga gccatccgga agatctggcg gccgctctcc
     4621 ctatagtgag tcgtattacg ccggatggat atggtgttca ggcacaagtg ttaaagcagt
     4681 tgattttatt cactatgatg aaaaaaacaa tgaatggaac ctgctccaag ttaaaaatag
     4741 agataatacc gaaaactcat cgagtagtaa gattagagat aatacaacaa taaaaaaatg
     4801 gtttagaact tactcacagc gtgatgctac taattgggac aattttccag atgaagtatc
     4861 atctaagaat ttaaatgaag aagacttcag agcttttgtt aaaaattatt tggcaaaaat
     4921 aatataattc ggctgcaggg gcggcctcgt gatacgccta tttttatagg ttaatgtcat
     4981 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5041 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5101 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5161 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5221 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5281 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5341 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5401 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5461 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5521 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5581 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5641 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5701 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5761 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5821 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5881 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5941 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6001 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6061 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6121 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6181 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6241 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6301 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6361 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6421 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6481 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6541 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6601 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6661 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6721 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6781 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6841 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6901 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aacc
//
