LOCUS       sequence-235973-        2783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for deCFP (cyan fluorescent 
            protein) - Please see Supplemental Documents for annotated Genbank 
            file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2783)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C9m
FEATURES             Location/Qualifiers
     source          1..2783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..67
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    73..93
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(123..144)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     472..491
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     731..752
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    798..819
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      822..865
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(869..887)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        955..1059
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1060..1128,1129..1920)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1278..1297)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2091..2679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2580..2599
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
       61 gagcgaggaa gcctgcagcg gccgctacta gtaggtctct acctttagat cccggcggcg
      121 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
      181 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
      241 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
      301 aagttggcct tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgatg
      361 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
      421 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
      481 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
      541 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccccag
      601 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
      661 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
      721 tggccgttta cgtcgccgtc cagctcgacc aggatgggaa caacgccagt gaaaagctcc
      781 atttgagacc cactagtctc tagaagcggc cgcgaattcc agaaatcatc cttagcgaaa
      841 gctaaggatt ttttttatct gaaattctgc ctcgtgatac gcctattttt ataggttaat
      901 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      961 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1021 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1081 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1141 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1201 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1261 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     1321 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1381 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1441 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1501 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1561 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1621 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1681 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     1741 tttattgctg ataaatctgg agccggtgag cgtgggtcgc gcggtatcat tgcagcactg
     1801 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     1861 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     1921 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     1981 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2041 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2101 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2161 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2221 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2281 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2341 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2401 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2461 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2521 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2581 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2641 tttttgtgat gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa cgcggccttt
     2701 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2761 gattctgtgg ataaccgtat tac
//
