LOCUS       sequence-235979-        2861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP 
            with ssrA degradation tag; variant of 
            https://www.fpbase.org/protein/yfp/).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2861)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C20m
FEATURES             Location/Qualifiers
     source          1..2861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    740..760
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(766..783)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(937..1525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1017..1036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1696..2487,2488..2556))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2319..2338
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2557..2661)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2729..2747
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2751..2794)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2797..2818
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             join(2834..2861,1..686)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc
       61 gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta
      121 ctggtaaact gccggtacct tggccgactc tggtaacgac gctgacttat ggtgttcagt
      181 gctttgctcg ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg
      241 aaggctatgt gcaggaacgc acgatttcct ttaaggatga cggcacgtac aaaacgcgtg
      301 cggaagtgaa atttgaaggc gataccctgg taaaccgcat tgagctgaaa ggcattgact
      361 ttaaagaaga cggcaatatc ctgggccata agctggaata caattttaac agccacaatg
      421 tttacatcac cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca
      481 acgtggagga tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg
      541 atggtcctgt tctgctgcca gacaatcact atctgagcta ccaaagcgtt ctgtctaaag
      601 atccgaacga gaaacgcgat catatggttc tgctggagtt cgtaaccgca gcgggcatca
      661 cgcatggtat ggatgaactg tacaaaacta gtgcagcgaa cgacgaaaat tacgcccttg
      721 cagcgtgaag gtagagacct actagtagcg gccgctgcag gcttcctcgc tcactgactc
      781 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      841 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      901 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga
      961 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1021 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1081 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1141 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1201 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1261 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1321 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1381 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1441 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1501 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1561 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1621 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1681 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1741 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1801 cttaccatct ggccccagtg ctgcaatgat accgcgcgac ccacgctcac cggctccaga
     1861 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1921 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1981 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2041 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2101 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2161 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2221 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2281 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2341 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2401 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2461 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2521 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2581 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2641 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     2701 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
     2761 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagac
     2821 tagtgggtct caaatgcgta aaggcgaaga gctgttcact g
//
