LOCUS       sequence-235982-        5175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Foxf1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Hoggatt AM, Kim JR, Ustiyan V, Ren X, Kalin TV, Kalinichenko VV, 
            Herring BP
  TITLE     The transcription factor Foxf1 binds to serum response factor and 
            myocardin to regulate gene transcription in visceral smooth muscle 
            cells.
  JOURNAL   J Biol Chem. 2013 Oct 4;288(40):28477-87. doi: 
            10.1074/jbc.M113.478974. Epub 2013 Aug 14.
  PUBMED    23946491
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.478974"; journalName: "J Biol Chem"; date: 
            "2013-10-4- 4"; volume: "288"; issue: "40"; pages: "28477-87"
COMMENT     Sequence Label: Foxf1 HA pShuttle
FEATURES             Location/Qualifiers
     source          1..5175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             670..696
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1803..1820)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1809..2033
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2198..2215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2369..2957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2449..2468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3654..4469)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3654..4469)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4377..4396
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4461..4480
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(4985..5004)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcatgt acccatacga tgttccagat tacgctgggc cctctagaat ggaccccgcg
      721 gcggcgggcc ccaccaaggc caagaagacc aacgccggcg tgcggcggcc ggagaagccg
      781 ccctactcgt acatcgcgct catcgtcatg gctatccaga gctcgcccag caagcgcctg
      841 accctcagcg agatctacca gtttcttcag gcgcgcttcc ccttcttccg cggcgcctac
      901 cagggctgga agaactccgt gcgccacaac ctgtcgctca acgagtgctt catcaaactg
      961 cccaagggcc tcgggcgacc cggcaagggc cactactgga ccatcgatcc ggctagcgag
     1021 tttatgttcg aggagggctc gttccgccgg cggccgcgcg gcttccgaag gaaatgccag
     1081 gcgctcaagc ctgtgtacag catggtgaac gggctgggct tcaaccacct ccccgacacc
     1141 tacggcttcc agggctccgg aggcctctcg tgcgcgccca acagcctggc gctggagggc
     1201 ggcttgggca tgatgaatgg ccatttggct ggcaacgtgg acggcatggc tttgcccagc
     1261 cactcggtgc cacacctgcc ctccaacggc ggccactcgt acatgggtgg ctgcggtggc
     1321 tctgcggccg gggagtaccc gcaccacgac agctcggtgc ccgcttcacc gctgctgccg
     1381 gccggcgccg gcggagtcat ggagccgcac gccgtttact ccagctctgc agcagcctgg
     1441 ccgcccgcgg cctccgcggc tctcaacagc ggggcctcct acatcaagca acagcctctg
     1501 tccccttgca acccagccgc caaccccctg tctggcagca tctccacgca ctccctggag
     1561 cagccatacc ttcaccaaaa cagtcacaac gggccggcag aactgcaagg catccctcgg
     1621 tatcactcac agtcgcccag catgtgtgac agaaaggagt ttgtcttctc tttcaatgcc
     1681 atggcctctt cttctatgca tacaacaggc ggaggatctt actatcacca gcaggtcacc
     1741 taccaagaca tcaagccgtg tgtgatgtga ggtaccaagc ttaagtttaa accgctgatc
     1801 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     1861 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     1921 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     1981 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     2041 ggcggaaaga accagcagat ctgcagatct gaattcatct atgtcgggtg cggagaaaga
     2101 ggtaatgaaa tggcattatg ggtattatgg gtctgcatta atgaatcggc caacgcgcgg
     2161 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2221 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2281 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2341 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2401 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2461 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2521 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2581 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2641 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2701 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2761 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2821 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2881 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2941 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3001 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3061 tccttttgat cctccggcgt tcagcctgtg ccacagccga caggatggtg accaccattt
     3121 gccccatatc accgtcggta ctgatcccgt cgtcaataaa ccgaaccgct acaccctgag
     3181 catcaaactc ttttatcagt tggatcatgt cggcggtgtc gcggccaaga cggtcgagct
     3241 tcttcaccag aatgacatca ccttcctcca ccttcatcct cagcaaatcc agcccttccc
     3301 gatctgttga actgccggat gccttgtcgg taaagatgcg gttagctttt acccctgcat
     3361 ctttgagcgc tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc aggcctgaat
     3421 cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt tgttgtaggt
     3481 ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt tgtcgggaag
     3541 atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa gccgccgtcc
     3601 cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc tgattagaaa
     3661 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     3721 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     3781 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     3841 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     3901 ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta
     3961 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     4021 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     4081 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     4141 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     4201 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     4261 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     4321 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     4381 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgag
     4441 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     4501 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     4561 tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc
     4621 acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac
     4681 tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat
     4741 ggggcgattc aggcctggta tgagtcagca acaccttctt cacgaggcag acctcagcgc
     4801 tagattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4861 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4921 taactataac ggtcctaagg tagcgaaagc tcagatctcc cgatccccta tggtgcactc
     4981 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     5041 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     5101 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     5161 ccagatatac gcgtt
//
