LOCUS       sequence-235983-       10739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning 
            homology arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 10739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1595
FEATURES             Location/Qualifiers
     source          1..10739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1887..1907
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1925..1958)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1968..1997
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3814..4137)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6055..6079
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6082..7107
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6082..7107
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7108..7132)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7485..7518)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7660..7791
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             7819..7842
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7846..7869
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7873..7896
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(7944..8249)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8518..8534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8518..8534)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8531..8553)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8542..8558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8566..8572,8573..8590,8591..8596))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8611..8632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8749..8766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8920..9508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9000..9019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9679..10470,10471..10539))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10302..10321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10540..10644)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10712..10730
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg gtctccgagc tcattaaaga aaacatgcat atgaagctct
     1081 acatggaggg aaccgtcaac aaccaccact tcaagtgcac ctccgaggga gagggaaagc
     1141 catacgaggg aacccaaacc atgcgtatca aggccgtcga gggaggacca ctcccattcg
     1201 ccttcgacat cctcgccacc tccttcatgt acggatccaa ggtaagttta aacatatata
     1261 tactaactaa ccctgattat ttaaattttc agaccttcat caaccacacc caaggaatcc
     1321 cagacttctt caagcaatcc ttcccagagg gattcacctg ggagcgtgtc accacctacg
     1381 aggacggagg agtcctcacc gccacccaag acacctccct ccaagacgga tgcctcatct
     1441 acaacgtcaa ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     1501 agatccgtgg agtcaacttc ccatccaacg gaccagtcat gcaaaagaag accctcggat
     1561 gggaggcctc caccgagacc ctctacccag ccgacggagg acttgaggga cgtgccgaca
     1621 tggccctcaa gctcgtcgga ggaggacacc tcatctgcaa cctcaaggta agtttaaaca
     1681 tgattttact aactaactaa tctgatttaa attttcagac cacctaccgt tccaagaagc
     1741 cagccaagaa cctcaagatg ccaggagtct actacgtcga ccgtcgtctt gagcgtatca
     1801 aggaggccga caaggagacc tacgtcgagc aacacgaggt cgccgtcgcc cgttactgcg
     1861 acctcccatc caagctcgga caccgtgaga atctgtactt tcaatccgga aaggtaagtt
     1921 taaaataact tcgtatagca tacattatac gaagttattt tcagggagag caaaagctta
     1981 tctccgagga ggacctctaa ggatgatcga cgccaacgtc gttgaatttt caaattttaa
     2041 atactgaata tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg
     2101 ctttctaaaa aattaattca atccaaatct aaacattttt ttttctcttt ccgtctccca
     2161 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt
     2221 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga
     2281 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt
     2341 aaaagaaaac ataaaaatat atagaggagg aaggaaacta gctgccctcg attgtctatc
     2401 ttccattctc accaagtttc aacaacttta ttcagtcatt cttctctggt tcatttccca
     2461 accaatcttc ccgaataaag cccatctgtc ctttcggcca cattcctcac ctacatccgt
     2521 actccttatc tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc acactgtctc
     2581 gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga tttcctaata
     2641 aaaggagacg aatccggtag gaaatcgaca agtctctgct tctcgaagac taggtgagtt
     2701 acatttcttt ggccgaatta ttttgaaatc ctaaatttga taatttcaga gatgtctgta
     2761 aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca ctcttatggt atgcttcatg
     2821 accatgtcaa ccatctactc ggaggttgat ggattcagag agaagcttga taccgagatg
     2881 aatgtgttca gagtgagtta aattatattt tgaattttaa ataattttta attttctagc
     2941 aatctaccaa tggattgtgg aaggacatag ttgtcatcgg aagatctagc aagcgtgtcc
     3001 gttgtcaata tgaagagacc aacgctaccc caactccaca tgctgatgga tccccatctg
     3061 ctccaccagg tcaaccacca gcagttccac cagtcttcaa ccagccaaag actccaaatg
     3121 gagccaatgg aaatggacca acctgcaact gcaatgctga taacaagtgc ccagctggac
     3181 catccggacc aaagggagtt ccaggagttc caggactcga cggagttcca ggacttgacg
     3241 gtgttccagg agttggagct gatgatatcg ctccacaacg cgagtctgtc ggatgcttca
     3301 cttgcccaca aggaccagtt ggaccaccag gagctcttgg aagaccagga ccacgtggac
     3361 ttccaggacc aagaggacaa aatggaaacc caggaagaga tggacaacca ggacatccag
     3421 gagagcaagg atcatccggt caaatcggaa agatcggaga gccaggacca ccaggagaga
     3481 agggacgcga cgccgagcat ccaatcggaa gaccaggacc aaagggacca agaggagatc
     3541 aaggaccaac aggaccagct ggacagaacg gtcttcacgg accaccagga gagccaggaa
     3601 ccgttggacc agaaggacca tctggaaagc aaggacgtca aggaccagac ggaacccagg
     3661 gagagactgg accagacgga agaccaggaa aggatgccga gtactgccag tgcccagaca
     3721 agtctccacc atcagaggct gtcaacgcca accgtggata cagaaatatc taaattgttg
     3781 gtgttttcta ataaaatatt tgagattcag tgagactttt ttcttggcgg cacaataaag
     3841 tttcctcttt ttgttgggtc actcaacgaa cacaatacaa atatttgagc gctcgcattt
     3901 ttctcaacct ttcgggttta aagcacttca aaatggaatt gaagagagat tgtgaaagta
     3961 catcagacag aatcacattt tgatagcatt cacttcactc agatgcaaac ttttcagtga
     4021 ctataaaaag tcataaaata ataacattaa aaaagcaaat aaattagcga gagaattttt
     4081 tgacaaaaag aaagaagagt gatagagaag aagggaatgc ttgaaaggat tttgcattta
     4141 tcttaatcac cgtcttcgag aagacgaacc atggcgccag tttcagaatc caagtttcta
     4201 atatagttca tcacaatgtt gacgttggtc caacctccgg cttgcataat ctctgggatg
     4261 gaaactccag cgcgagccat gtcacgggca gctccgacac gggcggaatg tccagaccaa
     4321 gccaagtaac gttgtccgga atcatccttg gctccgtaga tgagacggtg ggtagcttcg
     4381 aagattcctg aaaatttatt ttcagcttat tcttagctaa tatgttgttt aaacttacct
     4441 tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc gttcttgcga
     4501 acacgacaga acaggtagtt gtttggatca tcggcgactc cggaaaccga aatccagcgc
     4561 tcaacgagct tggtaactcc aagggagagc gctttttcga ctccggcagt cgagaccagc
     4621 gtttttgtac gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact aatatccttg
     4681 acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat tccgaggaag
     4741 gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca taagagaacg gacctgatcg
     4801 aagtcggttc tgaaaatttc gtcaagtctt aggattgaat acattaatat ttaaacttac
     4861 ctttcgaagg cgagggcttg cttggctctc tctccagcat caacattttc cttgcggata
     4921 cgtctcatta cgagagatac ggcattagaa tctgatgggc gtggaagtcc agatcggcga
     4981 tggagcatat tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa tccacgagct
     5041 tggaggtaga gcaagtagtc acgaacgtct tctggctcgg ctgggaacca cttgcggttg
     5101 ttaagcttgc accaggcagc ccaagaacgg cacactgaga gaagcatctt ccaggtgtgc
     5161 tcggagaagg cttgtctgtc acggaacata tccatgaggt tcttgcgaac ttcatcactt
     5221 gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag tgagaagatt tgacatattt
     5281 tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc ccgctttcct
     5341 tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat gcattcgttt
     5401 gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg gtctctttat
     5461 tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca acaagcagct
     5521 cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa ataccgaaca
     5581 acatttgctc taattgtgaa attagaaatc ttcaaactat aatcatgtca ctttaccact
     5641 atttccgtcc agctcaacgt tccgtttttg gtgatcattt ttgctttcgt cgtaaatcta
     5701 cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc aaattcgttg actgagtatt
     5761 caacgtttat acgttgtcgg caacgagaaa taggaaaatg catcgggaaa tgttcttttt
     5821 tcgatttttt ccaaggtttt gacaaatttt accacgaatt ttgctatgtt ttcaattaaa
     5881 aaatatgtta ttcaactgtt tctatgagga aaataaggct ttgcatgtaa ttttcttatt
     5941 cagcataatt tttaattaat ttgaattttc tgtcctaacg tttattttgt tttcttggtt
     6001 atgactgatc tgaaattaat ttttgaattt taaggtaata tgtcaggcgg tgccgcaagt
     6061 ttgtacaaaa aagcaggctc catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     6121 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     6181 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     6241 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     6301 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     6361 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     6421 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     6481 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     6541 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     6601 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     6661 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     6721 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     6781 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     6841 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     6901 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     6961 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     7021 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     7081 agcactcgtc cgagggcaaa ggaatagacc cagctttctt gtacaaagtg ggtccaatta
     7141 ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta
     7201 tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg
     7261 aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct
     7321 ccctcccccc attaataata attctatccc aaaatctaca caatgttctg tgtacacttc
     7381 ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa
     7441 taccttatca tatgttacgt ttcagtttat gaccgcaatt tttaataact tcgtatagca
     7501 tacattatac gaagttattt tcagggagcc ggatctgatt acggatccgg aggtggcggg
     7561 atggcttctt ctcttcgtca gatcctcgac tctcagaaga tggagtggcg ttctaacgct
     7621 ggaggatctg gcggaggagg cagtggtagt ggcagtatgc ctaaagatcc agccaaacct
     7681 ccggccaagg cacaagttgt gggatggcca ccggtgagat cataccggaa gaacgtgatg
     7741 gtttcctgcc aaaaatcaag cggtggcccg gaggcggcgg cgttcgtgaa gggtggtggc
     7801 ggttctggct caggatctga ttataaagac gatgacgata agcgtgacta caaggacgac
     7861 gacgacaagc gtgattacaa ggatgacgat gacaagagac tagttcgacc tgcagactgg
     7921 ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg
     7981 atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga gaccggcaca
     8041 ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa
     8101 agttcacggg ggactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt
     8161 cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata acggctctct
     8221 cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt cggcaaaaga
     8281 gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca
     8341 gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttaccg aaccggagat
     8401 attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata
     8461 cgctgcttca tagcatacct ctttttgaca tacttcgggt tcctaggaaa tcgataagtc
     8521 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8581 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8641 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8701 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8761 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8821 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8941 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9001 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9061 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9121 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9181 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9241 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9301 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9361 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9421 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9481 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9541 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9601 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9661 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9721 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9781 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9841 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9901 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9961 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10021 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10081 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10141 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10201 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10261 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10321 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10381 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10441 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10501 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10561 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10621 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10681 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
